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Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis.
Oral Dis. 2007 Nov; 13(6):594-9.OD

Abstract

AIM

This study evaluated whether diabetes modulates gene expression [interleukin (IL)-1beta, IL-1ra, IL-6, IL-8, IL-10; tumor necrosis factor (TNF)-alpha; interferon (IFN)-gamma, receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG)] in sites with periodontitis.

MATERIALS AND METHODS

Gingival biopsies were harvested and divided into three groups--Control group: systemically and periodontally healthy subjects (n = 10); Periodontitis group: systemically healthy subjects diagnosed with chronic periodontitis (n = 20); Diabetes group: type 1 diabetic subjects, diagnosed with chronic periodontitis (n = 20). Total RNA was obtained and analyzed by quantitative polymerase chain reaction.

RESULTS

Data analysis demonstrated that, except for OPG, mRNA levels for all factors were increased by inflammation (P < 0.001). Interleukin-1beta, IL-1ra, IL-6, IL-8, IFN-gamma, and RANKL mRNA levels were higher in the diabetic group when compared with the control non-periodontitis group (P < 0.05), whereas IL-10 and OPG were lower (P < 0.05). No difference was observed for TNF-alpha between diabetic and control groups (P > 0.05). Diabetes lowered IL-1beta, IL-8, IL-10, TNF-alpha, RANKL, and OPG mRNA levels in sites with comparable type of periodontitis (P < 0.001). Moreover, increased RANKL:OPG and IL-6:IL-10 ratios were found.

CONCLUSION AND CLINICAL RELEVANCE

Taken together, these data suggest that decreased levels of IL-10 and OPG may play an important role in the periodontal breakdown in diabetic patients.

Authors+Show Affiliations

Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17944678

Citation

Duarte, P M., et al. "Diabetes Modulates Gene Expression in the Gingival Tissues of Patients With Chronic Periodontitis." Oral Diseases, vol. 13, no. 6, 2007, pp. 594-9.
Duarte PM, Neto JB, Casati MZ, et al. Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis. Oral Dis. 2007;13(6):594-9.
Duarte, P. M., Neto, J. B., Casati, M. Z., Sallum, E. A., & Nociti, F. H. (2007). Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis. Oral Diseases, 13(6), 594-9.
Duarte PM, et al. Diabetes Modulates Gene Expression in the Gingival Tissues of Patients With Chronic Periodontitis. Oral Dis. 2007;13(6):594-9. PubMed PMID: 17944678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis. AU - Duarte,P M, AU - Neto,J B C, AU - Casati,M Z, AU - Sallum,E A, AU - Nociti,F H,Jr PY - 2007/10/20/pubmed PY - 2008/1/24/medline PY - 2007/10/20/entrez SP - 594 EP - 9 JF - Oral diseases JO - Oral Dis VL - 13 IS - 6 N2 - AIM: This study evaluated whether diabetes modulates gene expression [interleukin (IL)-1beta, IL-1ra, IL-6, IL-8, IL-10; tumor necrosis factor (TNF)-alpha; interferon (IFN)-gamma, receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG)] in sites with periodontitis. MATERIALS AND METHODS: Gingival biopsies were harvested and divided into three groups--Control group: systemically and periodontally healthy subjects (n = 10); Periodontitis group: systemically healthy subjects diagnosed with chronic periodontitis (n = 20); Diabetes group: type 1 diabetic subjects, diagnosed with chronic periodontitis (n = 20). Total RNA was obtained and analyzed by quantitative polymerase chain reaction. RESULTS: Data analysis demonstrated that, except for OPG, mRNA levels for all factors were increased by inflammation (P < 0.001). Interleukin-1beta, IL-1ra, IL-6, IL-8, IFN-gamma, and RANKL mRNA levels were higher in the diabetic group when compared with the control non-periodontitis group (P < 0.05), whereas IL-10 and OPG were lower (P < 0.05). No difference was observed for TNF-alpha between diabetic and control groups (P > 0.05). Diabetes lowered IL-1beta, IL-8, IL-10, TNF-alpha, RANKL, and OPG mRNA levels in sites with comparable type of periodontitis (P < 0.001). Moreover, increased RANKL:OPG and IL-6:IL-10 ratios were found. CONCLUSION AND CLINICAL RELEVANCE: Taken together, these data suggest that decreased levels of IL-10 and OPG may play an important role in the periodontal breakdown in diabetic patients. SN - 1354-523X UR - https://www.unboundmedicine.com/medline/citation/17944678/Diabetes_modulates_gene_expression_in_the_gingival_tissues_of_patients_with_chronic_periodontitis_ L2 - https://doi.org/10.1111/j.1601-0825.2006.01348.x DB - PRIME DP - Unbound Medicine ER -