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Comparison of newly synthetic hexaploid wheat with its donors on SSR products.
J Genet Genomics. 2007 Oct; 34(10):939-46.JG

Abstract

Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers. However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome of Ae. tauschii. Most amplified fragments from Ae. tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17945172

Citation

Zhang, Lianquan, et al. "Comparison of Newly Synthetic Hexaploid Wheat With Its Donors On SSR Products." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 34, no. 10, 2007, pp. 939-46.
Zhang L, Sun G, Yan Z, et al. Comparison of newly synthetic hexaploid wheat with its donors on SSR products. J Genet Genomics. 2007;34(10):939-46.
Zhang, L., Sun, G., Yan, Z., Chen, Q., Yuan, Z., Lan, X., Zheng, Y., & Liu, D. (2007). Comparison of newly synthetic hexaploid wheat with its donors on SSR products. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 34(10), 939-46.
Zhang L, et al. Comparison of Newly Synthetic Hexaploid Wheat With Its Donors On SSR Products. J Genet Genomics. 2007;34(10):939-46. PubMed PMID: 17945172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of newly synthetic hexaploid wheat with its donors on SSR products. AU - Zhang,Lianquan, AU - Sun,Genlou, AU - Yan,Zehong, AU - Chen,Qijiao, AU - Yuan,Zhongwei, AU - Lan,Xiujin, AU - Zheng,Youliang, AU - Liu,Dengcai, PY - 2007/01/26/received PY - 2007/03/21/accepted PY - 2007/10/20/pubmed PY - 2009/4/25/medline PY - 2007/10/20/entrez SP - 939 EP - 46 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 34 IS - 10 N2 - Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers. However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome of Ae. tauschii. Most amplified fragments from Ae. tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/17945172/Comparison_of_newly_synthetic_hexaploid_wheat_with_its_donors_on_SSR_products_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(07)60105-2 DB - PRIME DP - Unbound Medicine ER -