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An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection.
Planta Med. 2007 Nov; 73(14):1464-8.PM

Abstract

The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity.

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Authors+Show Affiliations

Talarico LB
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Duarte ME
No affiliation info available
Zibetti RG
No affiliation info available
Noseda MD
No affiliation info available
Damonte EB
No affiliation info available

MeSH

AnimalsAntiviral AgentsChlorocebus aethiopsDengue VirusDose-Response Relationship, DrugEukaryotaGalactansMolecular StructureVero Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17948168

Citation

Talarico, Laura B., et al. "An Algal-derived DL-galactan Hybrid Is an Efficient Preventing Agent for in Vitro Dengue Virus Infection." Planta Medica, vol. 73, no. 14, 2007, pp. 1464-8.
Talarico LB, Duarte ME, Zibetti RG, et al. An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection. Planta Med. 2007;73(14):1464-8.
Talarico, L. B., Duarte, M. E., Zibetti, R. G., Noseda, M. D., & Damonte, E. B. (2007). An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection. Planta Medica, 73(14), 1464-8.
Talarico LB, et al. An Algal-derived DL-galactan Hybrid Is an Efficient Preventing Agent for in Vitro Dengue Virus Infection. Planta Med. 2007;73(14):1464-8. PubMed PMID: 17948168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection. AU - Talarico,Laura B, AU - Duarte,María E, AU - Zibetti,Rosiane G, AU - Noseda,Miguel D, AU - Damonte,Elsa B, Y1 - 2007/10/18/ PY - 2007/10/20/pubmed PY - 2008/2/29/medline PY - 2007/10/20/entrez SP - 1464 EP - 8 JF - Planta medica JO - Planta Med VL - 73 IS - 14 N2 - The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/17948168/An_algal_derived_DL_galactan_hybrid_is_an_efficient_preventing_agent_for_in_vitro_dengue_virus_infection_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-990241 DB - PRIME DP - Unbound Medicine ER -