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Gram-scale purification of flavonolignan diastereoisomers from Silybum marianum (Milk Thistle) extract in support of preclinical in vivo studies for prostate cancer chemoprevention.
Planta Med 2007; 73(14):1495-501PM

Abstract

Extracts of milk thistle (Silybum marianum, Asteraceae), termed "silymarin," are used worldwide, primarily for hepatoprotective applications and recently for prostate cancer chemoprevention. Silymarin is a mixture of at least eight compounds, and four major constituents are a group of structurally related flavonolignans: silybin A, silybin B, isosilybin A, and isosilybin B. The initiation of IN VIVO studies to compare the respective preclinical activities of each compound required that gram quantities of these diastereoisomers be prepared. Procedures were developed and optimized to produce multigram-scale quantities of each of these in > 97 % purity. A hybrid chromatographic/precipitative technique was developed, whereby mixtures were chromatographed at high concentrations so as to induce formation of a precipitate in the column fractions, yielding samples that were more enriched in the desired compounds than would be obtained solely by the chromatographic steps alone.

Authors+Show Affiliations

Natural Products Laboratory, Research Triangle Institute, Research Triangle Park, NC 27709-2194, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17948171

Citation

Graf, Tyler N., et al. "Gram-scale Purification of Flavonolignan Diastereoisomers From Silybum Marianum (Milk Thistle) Extract in Support of Preclinical in Vivo Studies for Prostate Cancer Chemoprevention." Planta Medica, vol. 73, no. 14, 2007, pp. 1495-501.
Graf TN, Wani MC, Agarwal R, et al. Gram-scale purification of flavonolignan diastereoisomers from Silybum marianum (Milk Thistle) extract in support of preclinical in vivo studies for prostate cancer chemoprevention. Planta Med. 2007;73(14):1495-501.
Graf, T. N., Wani, M. C., Agarwal, R., Kroll, D. J., & Oberlies, N. H. (2007). Gram-scale purification of flavonolignan diastereoisomers from Silybum marianum (Milk Thistle) extract in support of preclinical in vivo studies for prostate cancer chemoprevention. Planta Medica, 73(14), pp. 1495-501.
Graf TN, et al. Gram-scale Purification of Flavonolignan Diastereoisomers From Silybum Marianum (Milk Thistle) Extract in Support of Preclinical in Vivo Studies for Prostate Cancer Chemoprevention. Planta Med. 2007;73(14):1495-501. PubMed PMID: 17948171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gram-scale purification of flavonolignan diastereoisomers from Silybum marianum (Milk Thistle) extract in support of preclinical in vivo studies for prostate cancer chemoprevention. AU - Graf,Tyler N, AU - Wani,Mansukh C, AU - Agarwal,Rajesh, AU - Kroll,David J, AU - Oberlies,Nicholas H, Y1 - 2007/10/18/ PY - 2007/10/20/pubmed PY - 2008/2/29/medline PY - 2007/10/20/entrez SP - 1495 EP - 501 JF - Planta medica JO - Planta Med. VL - 73 IS - 14 N2 - Extracts of milk thistle (Silybum marianum, Asteraceae), termed "silymarin," are used worldwide, primarily for hepatoprotective applications and recently for prostate cancer chemoprevention. Silymarin is a mixture of at least eight compounds, and four major constituents are a group of structurally related flavonolignans: silybin A, silybin B, isosilybin A, and isosilybin B. The initiation of IN VIVO studies to compare the respective preclinical activities of each compound required that gram quantities of these diastereoisomers be prepared. Procedures were developed and optimized to produce multigram-scale quantities of each of these in > 97 % purity. A hybrid chromatographic/precipitative technique was developed, whereby mixtures were chromatographed at high concentrations so as to induce formation of a precipitate in the column fractions, yielding samples that were more enriched in the desired compounds than would be obtained solely by the chromatographic steps alone. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/17948171/Gram_scale_purification_of_flavonolignan_diastereoisomers_from_Silybum_marianum__Milk_Thistle__extract_in_support_of_preclinical_in_vivo_studies_for_prostate_cancer_chemoprevention_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-990239 DB - PRIME DP - Unbound Medicine ER -