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The A17 enhancer directs expression of Myf5 to muscle satellite cells but Mrf4 to myonuclei.
Dev Dyn. 2007 Dec; 236(12):3419-26.DD

Abstract

The myogenic regulatory factors, Myf5 and Mrf4, play key roles in the specification and differentiation of skeletal muscle, respectively. Many cis-acting regulatory elements at the Mrf4/Myf5 locus have been identified, including the A17 enhancer. During development, A17 in conjunction with the Mrf4 or Myf5 promoter, directs transgene expression either to embryonic or fetal muscles. We now show that this enhancer also regulates Mrf4/Myf5 transcription in the adult. A17 linked to the Myf5 promoter drives expression in muscle satellite cells, whereas with the Mrf4 promoter, A17 directs transgene expression to myonuclei. Interestingly, expression of A17-Mrf4-nlacZ transgenes in myonuclei varies between muscles, revealing muscle autonomous transcriptional regulation. During muscle repair, satellite cells are induced to proliferate and differentiate to provide new myonuclei. A17 directs Myf5 expression in satellite cell progeny while it only drives the Mrf4 promoter after differentiation. Importantly, therefore, this promoter-specific activity directed by A17 reflects aspects of the expression profiles of the endogenous Myf5 and Mrf4 genes.

Authors+Show Affiliations

Département de Biologie du Développement, CNRS URA 2578, Institut Pasteur, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17948300

Citation

Chang, Ted Hung-Tse, et al. "The A17 Enhancer Directs Expression of Myf5 to Muscle Satellite Cells but Mrf4 to Myonuclei." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 236, no. 12, 2007, pp. 3419-26.
Chang TH, Vincent SD, Buckingham ME, et al. The A17 enhancer directs expression of Myf5 to muscle satellite cells but Mrf4 to myonuclei. Dev Dyn. 2007;236(12):3419-26.
Chang, T. H., Vincent, S. D., Buckingham, M. E., & Zammit, P. S. (2007). The A17 enhancer directs expression of Myf5 to muscle satellite cells but Mrf4 to myonuclei. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 236(12), 3419-26.
Chang TH, et al. The A17 Enhancer Directs Expression of Myf5 to Muscle Satellite Cells but Mrf4 to Myonuclei. Dev Dyn. 2007;236(12):3419-26. PubMed PMID: 17948300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The A17 enhancer directs expression of Myf5 to muscle satellite cells but Mrf4 to myonuclei. AU - Chang,Ted Hung-Tse, AU - Vincent,Stéphane D, AU - Buckingham,Margaret E, AU - Zammit,Peter S, PY - 2007/10/20/pubmed PY - 2008/2/28/medline PY - 2007/10/20/entrez SP - 3419 EP - 26 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 236 IS - 12 N2 - The myogenic regulatory factors, Myf5 and Mrf4, play key roles in the specification and differentiation of skeletal muscle, respectively. Many cis-acting regulatory elements at the Mrf4/Myf5 locus have been identified, including the A17 enhancer. During development, A17 in conjunction with the Mrf4 or Myf5 promoter, directs transgene expression either to embryonic or fetal muscles. We now show that this enhancer also regulates Mrf4/Myf5 transcription in the adult. A17 linked to the Myf5 promoter drives expression in muscle satellite cells, whereas with the Mrf4 promoter, A17 directs transgene expression to myonuclei. Interestingly, expression of A17-Mrf4-nlacZ transgenes in myonuclei varies between muscles, revealing muscle autonomous transcriptional regulation. During muscle repair, satellite cells are induced to proliferate and differentiate to provide new myonuclei. A17 directs Myf5 expression in satellite cell progeny while it only drives the Mrf4 promoter after differentiation. Importantly, therefore, this promoter-specific activity directed by A17 reflects aspects of the expression profiles of the endogenous Myf5 and Mrf4 genes. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/17948300/The_A17_enhancer_directs_expression_of_Myf5_to_muscle_satellite_cells_but_Mrf4_to_myonuclei_ L2 - https://doi.org/10.1002/dvdy.21356 DB - PRIME DP - Unbound Medicine ER -