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Lung tissue and tumour-infiltrating T lymphocytes in patients with non-small cell lung carcinoma and chronic obstructive pulmonary disease (COPD): moderate/severe versus mild stage of COPD.
Scand J Immunol. 2007 Dec; 66(6):694-702.SJ

Abstract

Cytotoxic CD8+ T cells have been suggested to be key players in the pathogenesis of chronic obstructive pulmonary disease (COPD). We wanted to investigate the phenotype of lung tissue T lymphocytes (LTL) and tumour-infiltrating T lymphocytes (TIL) in smokers with peripheral non-small cell lung carcinoma (NSCLC) with moderate/severe versus mild COPD. Lung tissue and tumour samples were obtained from patients with moderate/severe stage of COPD (n = 10) and from patients with mild stage of COPD (n = 7) at lung resection for a solitary peripheral NSCLC, processed and analysed by flow cytometry. The flow-cytometric results showed that lung tissue T cells, regardless of the severity of COPD, were mostly of the activated phenotype, expressed the CXCR3 chemokine receptor characteristic of type 1 T cells, and did neither significantly differ in the expression of activation markers (CD69, CD25 and HLA-DR), differentiation markers (CD27 and CD28) and chemokine receptors (CXCR3 and CCR4) between the selected groups, nor showed any significant correlation with lung function measured as forced expiratory volume in 1 s (FEV1) or TLCO. Compared with LTL, a significantly greater proportion of TIL expressed the activation markers CD69 and CD25, but a lower proportion showed a fully differentiated CD27- 28- phenotype. We conclude that lung LTL patterns are similar in NSCLC patients with moderate/severe or mild stages of COPD, and are not significantly related to lung function. LTL and TIL possess different phenotype characteristics. The majority of tumour tissue T cells are activated, but it seems that their process of differentiation is incomplete.

Authors+Show Affiliations

University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia. mateja.marc@klinika-golnik.siNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17949407

Citation

Marc, M M., et al. "Lung Tissue and Tumour-infiltrating T Lymphocytes in Patients With Non-small Cell Lung Carcinoma and Chronic Obstructive Pulmonary Disease (COPD): Moderate/severe Versus Mild Stage of COPD." Scandinavian Journal of Immunology, vol. 66, no. 6, 2007, pp. 694-702.
Marc MM, Korosec P, Kern I, et al. Lung tissue and tumour-infiltrating T lymphocytes in patients with non-small cell lung carcinoma and chronic obstructive pulmonary disease (COPD): moderate/severe versus mild stage of COPD. Scand J Immunol. 2007;66(6):694-702.
Marc, M. M., Korosec, P., Kern, I., Sok, M., Ihan, A., & Kosnik, M. (2007). Lung tissue and tumour-infiltrating T lymphocytes in patients with non-small cell lung carcinoma and chronic obstructive pulmonary disease (COPD): moderate/severe versus mild stage of COPD. Scandinavian Journal of Immunology, 66(6), 694-702.
Marc MM, et al. Lung Tissue and Tumour-infiltrating T Lymphocytes in Patients With Non-small Cell Lung Carcinoma and Chronic Obstructive Pulmonary Disease (COPD): Moderate/severe Versus Mild Stage of COPD. Scand J Immunol. 2007;66(6):694-702. PubMed PMID: 17949407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lung tissue and tumour-infiltrating T lymphocytes in patients with non-small cell lung carcinoma and chronic obstructive pulmonary disease (COPD): moderate/severe versus mild stage of COPD. AU - Marc,M M, AU - Korosec,P, AU - Kern,I, AU - Sok,M, AU - Ihan,A, AU - Kosnik,M, Y1 - 2007/10/18/ PY - 2007/10/24/pubmed PY - 2008/1/16/medline PY - 2007/10/24/entrez SP - 694 EP - 702 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 66 IS - 6 N2 - Cytotoxic CD8+ T cells have been suggested to be key players in the pathogenesis of chronic obstructive pulmonary disease (COPD). We wanted to investigate the phenotype of lung tissue T lymphocytes (LTL) and tumour-infiltrating T lymphocytes (TIL) in smokers with peripheral non-small cell lung carcinoma (NSCLC) with moderate/severe versus mild COPD. Lung tissue and tumour samples were obtained from patients with moderate/severe stage of COPD (n = 10) and from patients with mild stage of COPD (n = 7) at lung resection for a solitary peripheral NSCLC, processed and analysed by flow cytometry. The flow-cytometric results showed that lung tissue T cells, regardless of the severity of COPD, were mostly of the activated phenotype, expressed the CXCR3 chemokine receptor characteristic of type 1 T cells, and did neither significantly differ in the expression of activation markers (CD69, CD25 and HLA-DR), differentiation markers (CD27 and CD28) and chemokine receptors (CXCR3 and CCR4) between the selected groups, nor showed any significant correlation with lung function measured as forced expiratory volume in 1 s (FEV1) or TLCO. Compared with LTL, a significantly greater proportion of TIL expressed the activation markers CD69 and CD25, but a lower proportion showed a fully differentiated CD27- 28- phenotype. We conclude that lung LTL patterns are similar in NSCLC patients with moderate/severe or mild stages of COPD, and are not significantly related to lung function. LTL and TIL possess different phenotype characteristics. The majority of tumour tissue T cells are activated, but it seems that their process of differentiation is incomplete. SN - 1365-3083 UR - https://www.unboundmedicine.com/medline/citation/17949407/Lung_tissue_and_tumour_infiltrating_T_lymphocytes_in_patients_with_non_small_cell_lung_carcinoma_and_chronic_obstructive_pulmonary_disease__COPD_:_moderate/severe_versus_mild_stage_of_COPD_ L2 - https://doi.org/10.1111/j.1365-3083.2007.02018.x DB - PRIME DP - Unbound Medicine ER -