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High diversity of Panton-Valentine leukocidin-positive, methicillin-susceptible isolates of Staphylococcus aureus and implications for the evolution of community-associated methicillin-resistant S. aureus.
Clin Microbiol Infect. 2007 Dec; 13(12):1157-64.CM

Abstract

In total, 100 Staphylococcus aureus isolates from diverse cases of skin and soft-tissue infection at a university hospital in Saxony, Germany, were characterised using diagnostic microarrays. Virulence factors, including Panton-Valentine leukocidin (PVL), were detected and the isolates were assigned to clonal groups. Thirty isolates were positive for the genes encoding PVL. Only three PVL-positive methicillin-resistant S. aureus (MRSA) isolates were found, two of which belonged to European clone ST80-MRSA IV and one to USA300 strain ST8-MRSA IV. The remaining methicillin-susceptible PVL-positive isolates belonged to a variety of different multilocus sequence types. The predominant strains were agrI/ST22, agrII/CC5, agrIII/CC30 and agrIV/ST121. In order to check for possible bias caused by regional or local outbreak strains, an additional 18 methicillin-susceptible, PVL-positive isolates from the UK were tested. Approximately two-thirds of the UK isolates belonged to types that also comprised approximately two-thirds of the isolates from Saxony. Some methicillin-susceptible PVL-positive isolates (agrI/ST152, agrIII/ST80 and agrIII/ST96) closely resembled known epidemic community-acquired MRSA (CaMRSA) strains. These findings indicate that the current rise in PVL-positive CaMRSA could be caused by the dissemination of novel SCCmec elements among pre-existing PVL-positive strains, rather than by the spread of PVL phages among MRSA strains.

Authors+Show Affiliations

Institute for Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, Technical University of Dresden, Dresden, Germany. monecke@rocketmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17949441

Citation

Monecke, S, et al. "High Diversity of Panton-Valentine Leukocidin-positive, Methicillin-susceptible Isolates of Staphylococcus Aureus and Implications for the Evolution of Community-associated Methicillin-resistant S. Aureus." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 13, no. 12, 2007, pp. 1157-64.
Monecke S, Slickers P, Ellington MJ, et al. High diversity of Panton-Valentine leukocidin-positive, methicillin-susceptible isolates of Staphylococcus aureus and implications for the evolution of community-associated methicillin-resistant S. aureus. Clin Microbiol Infect. 2007;13(12):1157-64.
Monecke, S., Slickers, P., Ellington, M. J., Kearns, A. M., & Ehricht, R. (2007). High diversity of Panton-Valentine leukocidin-positive, methicillin-susceptible isolates of Staphylococcus aureus and implications for the evolution of community-associated methicillin-resistant S. aureus. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 13(12), 1157-64.
Monecke S, et al. High Diversity of Panton-Valentine Leukocidin-positive, Methicillin-susceptible Isolates of Staphylococcus Aureus and Implications for the Evolution of Community-associated Methicillin-resistant S. Aureus. Clin Microbiol Infect. 2007;13(12):1157-64. PubMed PMID: 17949441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High diversity of Panton-Valentine leukocidin-positive, methicillin-susceptible isolates of Staphylococcus aureus and implications for the evolution of community-associated methicillin-resistant S. aureus. AU - Monecke,S, AU - Slickers,P, AU - Ellington,M J, AU - Kearns,A M, AU - Ehricht,R, Y1 - 2007/10/19/ PY - 2007/10/24/pubmed PY - 2008/1/18/medline PY - 2007/10/24/entrez SP - 1157 EP - 64 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 13 IS - 12 N2 - In total, 100 Staphylococcus aureus isolates from diverse cases of skin and soft-tissue infection at a university hospital in Saxony, Germany, were characterised using diagnostic microarrays. Virulence factors, including Panton-Valentine leukocidin (PVL), were detected and the isolates were assigned to clonal groups. Thirty isolates were positive for the genes encoding PVL. Only three PVL-positive methicillin-resistant S. aureus (MRSA) isolates were found, two of which belonged to European clone ST80-MRSA IV and one to USA300 strain ST8-MRSA IV. The remaining methicillin-susceptible PVL-positive isolates belonged to a variety of different multilocus sequence types. The predominant strains were agrI/ST22, agrII/CC5, agrIII/CC30 and agrIV/ST121. In order to check for possible bias caused by regional or local outbreak strains, an additional 18 methicillin-susceptible, PVL-positive isolates from the UK were tested. Approximately two-thirds of the UK isolates belonged to types that also comprised approximately two-thirds of the isolates from Saxony. Some methicillin-susceptible PVL-positive isolates (agrI/ST152, agrIII/ST80 and agrIII/ST96) closely resembled known epidemic community-acquired MRSA (CaMRSA) strains. These findings indicate that the current rise in PVL-positive CaMRSA could be caused by the dissemination of novel SCCmec elements among pre-existing PVL-positive strains, rather than by the spread of PVL phages among MRSA strains. SN - 1198-743X UR - https://www.unboundmedicine.com/medline/citation/17949441/High_diversity_of_Panton_Valentine_leukocidin_positive_methicillin_susceptible_isolates_of_Staphylococcus_aureus_and_implications_for_the_evolution_of_community_associated_methicillin_resistant_S__aureus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)62582-9 DB - PRIME DP - Unbound Medicine ER -