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Circulating levels of both Th1 and Th2 chemokines are elevated in patients with sarcoidosis.
Respir Med. 2008 Feb; 102(2):239-47.RM

Abstract

BACKGROUND

In sarcoidosis, the T helper type 1 (Th1) response tends to predominate at affected disease sites; however, whether Th1/Th2 polarization occurs in the peripheral circulation is unknown.

METHODS

Fifty-two patients with sarcoidosis and 21 healthy volunteers were investigated. The concentrations of interferon-inducible protein 10 (IP-10)/CXCL10 and thymus- and activation-regulated chemokine (TARC)/CCL17 in the serum, bronchoalveolar lavage fluid (BALF) and culture supernatant were measured by an enzyme-linked immunosorbent assay. The circulating CXCR3+ CD4+ T cells and CCR4+ CD4+ T cells were assessed by flow cytometry.

RESULTS

The CXCR3- or CCR4-positive ratios among CD4+ T cells were both higher in sarcoidosis than in healthy volunteers. The serum levels of both IP-10 and TARC of the patients with sarcoidosis were significantly higher than those of the healthy volunteers. In patients with sarcoidosis, a larger amount of IP-10 was generated by the BALF cells, whereas IP-10 production by peripheral blood mononuclear cells did not increase in comparison to the control subjects. The TARC levels produced by peripheral blood mononuclear cells of sarcoidosis patients were significantly higher than those of the controls, while no difference existed between the 2 groups regarding TARC production by BALF cells.

CONCLUSION

IP-10 is mainly produced at the lung and TARC in the peripheral circulation in sarcoidosis patients. Both IP-10 and TARC cooperatively play a role in the pathogenesis of sarcoidosis.

Authors+Show Affiliations

Division of Pulmonary Disease, Third Department of Internal Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan. nureki@med.oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17949965

Citation

Nureki, Shin-ichi, et al. "Circulating Levels of Both Th1 and Th2 Chemokines Are Elevated in Patients With Sarcoidosis." Respiratory Medicine, vol. 102, no. 2, 2008, pp. 239-47.
Nureki S, Miyazaki E, Ando M, et al. Circulating levels of both Th1 and Th2 chemokines are elevated in patients with sarcoidosis. Respir Med. 2008;102(2):239-47.
Nureki, S., Miyazaki, E., Ando, M., Ueno, T., Fukami, T., Kumamoto, T., Sugisaki, K., & Tsuda, T. (2008). Circulating levels of both Th1 and Th2 chemokines are elevated in patients with sarcoidosis. Respiratory Medicine, 102(2), 239-47.
Nureki S, et al. Circulating Levels of Both Th1 and Th2 Chemokines Are Elevated in Patients With Sarcoidosis. Respir Med. 2008;102(2):239-47. PubMed PMID: 17949965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating levels of both Th1 and Th2 chemokines are elevated in patients with sarcoidosis. AU - Nureki,Shin-ichi, AU - Miyazaki,Eishi, AU - Ando,Masaru, AU - Ueno,Takuya, AU - Fukami,Tetsujiro, AU - Kumamoto,Toshihide, AU - Sugisaki,Katsunori, AU - Tsuda,Tomiyasu, Y1 - 2007/10/18/ PY - 2007/03/15/received PY - 2007/09/09/revised PY - 2007/09/13/accepted PY - 2007/10/24/pubmed PY - 2008/8/22/medline PY - 2007/10/24/entrez SP - 239 EP - 47 JF - Respiratory medicine JO - Respir Med VL - 102 IS - 2 N2 - BACKGROUND: In sarcoidosis, the T helper type 1 (Th1) response tends to predominate at affected disease sites; however, whether Th1/Th2 polarization occurs in the peripheral circulation is unknown. METHODS: Fifty-two patients with sarcoidosis and 21 healthy volunteers were investigated. The concentrations of interferon-inducible protein 10 (IP-10)/CXCL10 and thymus- and activation-regulated chemokine (TARC)/CCL17 in the serum, bronchoalveolar lavage fluid (BALF) and culture supernatant were measured by an enzyme-linked immunosorbent assay. The circulating CXCR3+ CD4+ T cells and CCR4+ CD4+ T cells were assessed by flow cytometry. RESULTS: The CXCR3- or CCR4-positive ratios among CD4+ T cells were both higher in sarcoidosis than in healthy volunteers. The serum levels of both IP-10 and TARC of the patients with sarcoidosis were significantly higher than those of the healthy volunteers. In patients with sarcoidosis, a larger amount of IP-10 was generated by the BALF cells, whereas IP-10 production by peripheral blood mononuclear cells did not increase in comparison to the control subjects. The TARC levels produced by peripheral blood mononuclear cells of sarcoidosis patients were significantly higher than those of the controls, while no difference existed between the 2 groups regarding TARC production by BALF cells. CONCLUSION: IP-10 is mainly produced at the lung and TARC in the peripheral circulation in sarcoidosis patients. Both IP-10 and TARC cooperatively play a role in the pathogenesis of sarcoidosis. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/17949965/Circulating_levels_of_both_Th1_and_Th2_chemokines_are_elevated_in_patients_with_sarcoidosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(07)00393-9 DB - PRIME DP - Unbound Medicine ER -