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Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods.
J Appl Microbiol. 2007 Nov; 103(5):1657-68.JA

Abstract

AIM

To develop and validate high throughput methods for the direct enumeration of viable and culturable Salmonella and Escherichia coli O157:H7 in ground beef, carcass, hide and faecal (GCHF) samples from cattle.

METHODS AND RESULTS

The hydrophobic grid membrane filtration (HGMF) method and the spiral plate count method (SPCM) were evaluated as rapid tools for the estimation of pathogen load using GCHF samples spiked with known levels of Salmonella serotype Typhimurium. Validation studies showed that for a single determination of each sample type the low end of the detection limits were approx. 2.0 x 10(0) CFU g(-1) for ground beef, 5.0 x 10(-1) CFU (100 cm(2))(-1) for Salmonella and 8.0 x 10(-1) CFU (100 cm(2))(-1) for E. coli O157:H7 on carcasses, 4.0 x 10(1) CFU (100 cm(2))(-1) for hide and 2.0 x 10(2) CFU g(-1) for faecal samples. In addition, ground beef (n = 609), carcass (n = 1520) and hide (n = 3038) samples were collected from beef-processing plants and faecal samples (n = 3190) were collected from feed-lot cattle, and these samples were tested for the presence of Salmonella and E. coli O157:H7 by enrichment and enumeration methods.

CONCLUSIONS

The direct enumeration methods described here are amenable to high throughput sample processing and were found to be cost-effective alternatives to other enumeration methods for the estimation of Salmonella and E. coli O157:H7, in samples collected during cattle production and beef processing.

SIGNIFICANCE AND IMPACT OF THE STUDY

Use of the methods described here would allow for more routine testing and quantification data collection, providing useful information about the effectiveness of beef processing intervention strategies.

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Authors+Show Affiliations

Brichta-Harhay DM
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933-0166, USA. dayna_harhay@ars.usda.gov
Arthur TM
No affiliation info available
Bosilevac JM
No affiliation info available
Guerini MN
No affiliation info available
Kalchayanand N
No affiliation info available
Koohmaraie M
No affiliation info available

MeSH

AnimalsBacteriological TechniquesCattleColony Count, MicrobialCosts and Cost AnalysisEscherichia coli O157FecesFood MicrobiologyFood-Processing IndustryMeatMeat ProductsReproducibility of ResultsSalmonellaSalmonella InfectionsSkin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17953577

Citation

Brichta-Harhay, D M., et al. "Enumeration of Salmonella and Escherichia Coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Faecal Samples Using Direct Plating Methods." Journal of Applied Microbiology, vol. 103, no. 5, 2007, pp. 1657-68.
Brichta-Harhay DM, Arthur TM, Bosilevac JM, et al. Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. J Appl Microbiol. 2007;103(5):1657-68.
Brichta-Harhay, D. M., Arthur, T. M., Bosilevac, J. M., Guerini, M. N., Kalchayanand, N., & Koohmaraie, M. (2007). Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. Journal of Applied Microbiology, 103(5), 1657-68.
Brichta-Harhay DM, et al. Enumeration of Salmonella and Escherichia Coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Faecal Samples Using Direct Plating Methods. J Appl Microbiol. 2007;103(5):1657-68. PubMed PMID: 17953577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enumeration of Salmonella and Escherichia coli O157:H7 in ground beef, cattle carcass, hide and faecal samples using direct plating methods. AU - Brichta-Harhay,D M, AU - Arthur,T M, AU - Bosilevac,J M, AU - Guerini,M N, AU - Kalchayanand,N, AU - Koohmaraie,M, PY - 2007/10/24/pubmed PY - 2008/4/4/medline PY - 2007/10/24/entrez SP - 1657 EP - 68 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 103 IS - 5 N2 - AIM: To develop and validate high throughput methods for the direct enumeration of viable and culturable Salmonella and Escherichia coli O157:H7 in ground beef, carcass, hide and faecal (GCHF) samples from cattle. METHODS AND RESULTS: The hydrophobic grid membrane filtration (HGMF) method and the spiral plate count method (SPCM) were evaluated as rapid tools for the estimation of pathogen load using GCHF samples spiked with known levels of Salmonella serotype Typhimurium. Validation studies showed that for a single determination of each sample type the low end of the detection limits were approx. 2.0 x 10(0) CFU g(-1) for ground beef, 5.0 x 10(-1) CFU (100 cm(2))(-1) for Salmonella and 8.0 x 10(-1) CFU (100 cm(2))(-1) for E. coli O157:H7 on carcasses, 4.0 x 10(1) CFU (100 cm(2))(-1) for hide and 2.0 x 10(2) CFU g(-1) for faecal samples. In addition, ground beef (n = 609), carcass (n = 1520) and hide (n = 3038) samples were collected from beef-processing plants and faecal samples (n = 3190) were collected from feed-lot cattle, and these samples were tested for the presence of Salmonella and E. coli O157:H7 by enrichment and enumeration methods. CONCLUSIONS: The direct enumeration methods described here are amenable to high throughput sample processing and were found to be cost-effective alternatives to other enumeration methods for the estimation of Salmonella and E. coli O157:H7, in samples collected during cattle production and beef processing. SIGNIFICANCE AND IMPACT OF THE STUDY: Use of the methods described here would allow for more routine testing and quantification data collection, providing useful information about the effectiveness of beef processing intervention strategies. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/17953577/Enumeration_of_Salmonella_and_Escherichia_coli_O157:H7_in_ground_beef_cattle_carcass_hide_and_faecal_samples_using_direct_plating_methods_ L2 - https://doi.org/10.1111/j.1365-2672.2007.03405.x DB - PRIME DP - Unbound Medicine ER -