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Antibacterial and free-radical scavenging activities of Sonoran propolis.
J Appl Microbiol 2007; 103(5):1747-56JA

Abstract

AIMS

To evaluate the antibacterial and free-radical scavenging (FRS) activities of propolis collected from three different areas of Sonoran Desert in northwestern Mexico [Pueblo de Alamos (PAP), Ures (UP) and Caborca (CP)].

METHODS AND RESULTS

The antibacterial and FRS activities of Sonoran propolis were determined by the broth microdilution method and the DPPH (1,1-diphenyl-2-picrylhydracyl) assay, respectively. Propolis samples had antibacterial activity against only Gram-positive bacteria. The UP sample showed the highest antibacterial activity against Staphylococcus aureus [minimal inhibitory concentration (MIC) 100 microg ml(-1)] in a concentration-dependent manner (UP > CP > PAP). Caffeic acid phenethyl ester (CAPE), a UP propolis constituent, had very high growth-inhibitory activity towards Gram-positive bacteria, particularly against S. aureus (MIC 0.1 mmol l(-1)). To our knowledge, this is the first study showing a strong antibacterial activity of CAPE against S. aureus. Additionally, propolis CP exhibited high FRS activity (86% +/- 0.3 at 100 microg ml(-1)) comparable with those of the reference antioxidants vitamin C (87.4% +/- 1.7 at 70 micromol l(-1)) and BHT (66.07% +/- 0.76 at 140 micromol l(-1)). The propolis compounds CAPE and rutin showed high FRS activity (90.4% +/- 0.2 and 88.5% +/- 0.8 at 70 micromol l(-1), respectively).

CONCLUSIONS

Sonoran propolis UP and CAPE had strong antibacterial activity against S. aureus. In addition, propolis CP showed potent FRS activity comparable with those of vitamin C and BHT.

SIGNIFICANCE AND IMPACT OF THE STUDY

The strong antibacterial and antioxidant properties of Sonoran propolis and some of its constituents support further studies on the clinical applications of this natural bee product against S. aureus and several oxidative damage-related diseases.

Authors+Show Affiliations

Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17953585

Citation

Velazquez, C, et al. "Antibacterial and Free-radical Scavenging Activities of Sonoran Propolis." Journal of Applied Microbiology, vol. 103, no. 5, 2007, pp. 1747-56.
Velazquez C, Navarro M, Acosta A, et al. Antibacterial and free-radical scavenging activities of Sonoran propolis. J Appl Microbiol. 2007;103(5):1747-56.
Velazquez, C., Navarro, M., Acosta, A., Angulo, A., Dominguez, Z., Robles, R., ... Hernandez, J. (2007). Antibacterial and free-radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology, 103(5), pp. 1747-56.
Velazquez C, et al. Antibacterial and Free-radical Scavenging Activities of Sonoran Propolis. J Appl Microbiol. 2007;103(5):1747-56. PubMed PMID: 17953585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and free-radical scavenging activities of Sonoran propolis. AU - Velazquez,C, AU - Navarro,M, AU - Acosta,A, AU - Angulo,A, AU - Dominguez,Z, AU - Robles,R, AU - Robles-Zepeda,R, AU - Lugo,E, AU - Goycoolea,F M, AU - Velazquez,E F, AU - Astiazaran,H, AU - Hernandez,J, PY - 2007/10/24/pubmed PY - 2008/4/4/medline PY - 2007/10/24/entrez SP - 1747 EP - 56 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 103 IS - 5 N2 - AIMS: To evaluate the antibacterial and free-radical scavenging (FRS) activities of propolis collected from three different areas of Sonoran Desert in northwestern Mexico [Pueblo de Alamos (PAP), Ures (UP) and Caborca (CP)]. METHODS AND RESULTS: The antibacterial and FRS activities of Sonoran propolis were determined by the broth microdilution method and the DPPH (1,1-diphenyl-2-picrylhydracyl) assay, respectively. Propolis samples had antibacterial activity against only Gram-positive bacteria. The UP sample showed the highest antibacterial activity against Staphylococcus aureus [minimal inhibitory concentration (MIC) 100 microg ml(-1)] in a concentration-dependent manner (UP > CP > PAP). Caffeic acid phenethyl ester (CAPE), a UP propolis constituent, had very high growth-inhibitory activity towards Gram-positive bacteria, particularly against S. aureus (MIC 0.1 mmol l(-1)). To our knowledge, this is the first study showing a strong antibacterial activity of CAPE against S. aureus. Additionally, propolis CP exhibited high FRS activity (86% +/- 0.3 at 100 microg ml(-1)) comparable with those of the reference antioxidants vitamin C (87.4% +/- 1.7 at 70 micromol l(-1)) and BHT (66.07% +/- 0.76 at 140 micromol l(-1)). The propolis compounds CAPE and rutin showed high FRS activity (90.4% +/- 0.2 and 88.5% +/- 0.8 at 70 micromol l(-1), respectively). CONCLUSIONS: Sonoran propolis UP and CAPE had strong antibacterial activity against S. aureus. In addition, propolis CP showed potent FRS activity comparable with those of vitamin C and BHT. SIGNIFICANCE AND IMPACT OF THE STUDY: The strong antibacterial and antioxidant properties of Sonoran propolis and some of its constituents support further studies on the clinical applications of this natural bee product against S. aureus and several oxidative damage-related diseases. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/17953585/Antibacterial_and_free_radical_scavenging_activities_of_Sonoran_propolis_ L2 - https://doi.org/10.1111/j.1365-2672.2007.03409.x DB - PRIME DP - Unbound Medicine ER -