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Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression.
Clin Endocrinol (Oxf) 2007; 67(5):674-8CE

Abstract

OBJECTIVE

To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level.

PATIENTS AND DESIGN

A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy.

MEASUREMENTS

Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR).

RESULTS

Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group.

CONCLUSIONS

Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients.

Authors+Show Affiliations

3rd Department of Medicine, 1st Faculty of Medicine and General University Hospital, Prague, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17953628

Citation

Dolezalova, Radka, et al. "Changes of Endocrine Function of Adipose Tissue in Anorexia Nervosa: Comparison of Circulating Levels Versus Subcutaneous mRNA Expression." Clinical Endocrinology, vol. 67, no. 5, 2007, pp. 674-8.
Dolezalova R, Lacinova Z, Dolinkova M, et al. Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression. Clin Endocrinol (Oxf). 2007;67(5):674-8.
Dolezalova, R., Lacinova, Z., Dolinkova, M., Kleiblova, P., Haluzikova, D., Housa, D., ... Haluzik, M. (2007). Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression. Clinical Endocrinology, 67(5), pp. 674-8.
Dolezalova R, et al. Changes of Endocrine Function of Adipose Tissue in Anorexia Nervosa: Comparison of Circulating Levels Versus Subcutaneous mRNA Expression. Clin Endocrinol (Oxf). 2007;67(5):674-8. PubMed PMID: 17953628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression. AU - Dolezalova,Radka, AU - Lacinova,Zdena, AU - Dolinkova,Marketa, AU - Kleiblova,Petra, AU - Haluzikova,Denisa, AU - Housa,Daniel, AU - Papezova,Hana, AU - Haluzik,Martin, PY - 2007/10/24/pubmed PY - 2008/5/1/medline PY - 2007/10/24/entrez SP - 674 EP - 8 JF - Clinical endocrinology JO - Clin. Endocrinol. (Oxf) VL - 67 IS - 5 N2 - OBJECTIVE: To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level. PATIENTS AND DESIGN: A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy. MEASUREMENTS: Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR). RESULTS: Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group. CONCLUSIONS: Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients. SN - 0300-0664 UR - https://www.unboundmedicine.com/medline/citation/17953628/Changes_of_endocrine_function_of_adipose_tissue_in_anorexia_nervosa:_comparison_of_circulating_levels_versus_subcutaneous_mRNA_expression_ L2 - https://doi.org/10.1111/j.1365-2265.2007.02944.x DB - PRIME DP - Unbound Medicine ER -