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LXRalpha enhances lipid synthesis in SZ95 sebocytes.
J Invest Dermatol. 2008 May; 128(5):1266-72.JI

Abstract

Differentiation of sebocytes is strongly associated with enhanced lipid synthesis and accumulation in the cells. Liver X receptors (LXRs) are members of the nuclear receptor superfamily, which play a critical role in cholesterol homeostasis and lipid metabolism. We examined whether LXRalpha regulated lipid synthesis in the immortalized human sebaceous gland cell line SZ95. When the SZ95 sebocytes were treated with the ligand of LXR such as TO901317 or 22(R)-hydroxycholesterol, lipid droplets were accumulated in the majority of cells when examined by Oil Red O staining. The expression of the known LXR targets, such as fatty acid synthase and sterol regulatory-binding protein-1, was induced by TO901317. TO901317 treatment increased expression of LXRalpha but not that of LXRbeta. Transfection of antisense LXRalpha significantly decreased TO901317-induced target gene expression and lipid droplet accumulation, suggesting a major role of LXRalpha in differentiation of sebocytes. Further, TO901317 decreased the expression of cyclooxygenase-2 and inducible nitric oxide synthase that was induced by lipopolysaccharide treatment. Together, these results indicate that important roles of LXRalpha in differentiation and inflammatory signaling in sebaceous glands. Thus, we suggest that LXR ligands could provide a new class of therapeutic agents for sebaceous gland-associated disorders such as seborrhea and acne.

Authors+Show Affiliations

College of Pharmacy and Bio-MAX Institute, Seoul National University, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17960176

Citation

Hong, Il, et al. "LXRalpha Enhances Lipid Synthesis in SZ95 Sebocytes." The Journal of Investigative Dermatology, vol. 128, no. 5, 2008, pp. 1266-72.
Hong I, Lee MH, Na TY, et al. LXRalpha enhances lipid synthesis in SZ95 sebocytes. J Invest Dermatol. 2008;128(5):1266-72.
Hong, I., Lee, M. H., Na, T. Y., Zouboulis, C. C., & Lee, M. O. (2008). LXRalpha enhances lipid synthesis in SZ95 sebocytes. The Journal of Investigative Dermatology, 128(5), 1266-72.
Hong I, et al. LXRalpha Enhances Lipid Synthesis in SZ95 Sebocytes. J Invest Dermatol. 2008;128(5):1266-72. PubMed PMID: 17960176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LXRalpha enhances lipid synthesis in SZ95 sebocytes. AU - Hong,Il, AU - Lee,Min-Ho, AU - Na,Tae-Young, AU - Zouboulis,Christos C, AU - Lee,Mi-Ock, Y1 - 2007/10/25/ PY - 2007/10/26/pubmed PY - 2008/5/20/medline PY - 2007/10/26/entrez SP - 1266 EP - 72 JF - The Journal of investigative dermatology JO - J. Invest. Dermatol. VL - 128 IS - 5 N2 - Differentiation of sebocytes is strongly associated with enhanced lipid synthesis and accumulation in the cells. Liver X receptors (LXRs) are members of the nuclear receptor superfamily, which play a critical role in cholesterol homeostasis and lipid metabolism. We examined whether LXRalpha regulated lipid synthesis in the immortalized human sebaceous gland cell line SZ95. When the SZ95 sebocytes were treated with the ligand of LXR such as TO901317 or 22(R)-hydroxycholesterol, lipid droplets were accumulated in the majority of cells when examined by Oil Red O staining. The expression of the known LXR targets, such as fatty acid synthase and sterol regulatory-binding protein-1, was induced by TO901317. TO901317 treatment increased expression of LXRalpha but not that of LXRbeta. Transfection of antisense LXRalpha significantly decreased TO901317-induced target gene expression and lipid droplet accumulation, suggesting a major role of LXRalpha in differentiation of sebocytes. Further, TO901317 decreased the expression of cyclooxygenase-2 and inducible nitric oxide synthase that was induced by lipopolysaccharide treatment. Together, these results indicate that important roles of LXRalpha in differentiation and inflammatory signaling in sebaceous glands. Thus, we suggest that LXR ligands could provide a new class of therapeutic agents for sebaceous gland-associated disorders such as seborrhea and acne. SN - 1523-1747 UR - https://www.unboundmedicine.com/medline/citation/17960176/LXRalpha_enhances_lipid_synthesis_in_SZ95_sebocytes_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)33833-1 DB - PRIME DP - Unbound Medicine ER -