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A role for the myogenic determination gene Myf5 in adult regenerative myogenesis.
Dev Biol. 2007 Dec 01; 312(1):13-28.DB

Abstract

The myogenic determination genes Myf5, Myod and Mrf4 direct skeletal muscle cell fate prenatally. In adult myogenesis, Myod has been shown to regulate myoblast differentiation, however, our understanding of satellite cell regulation is incomplete since the roles of Myf5 and Mrf4 had not been clearly defined. Here we examine the function of Myf5 and Mrf4 in the adult using recently generated alleles. Mrf4 is not expressed in normal or Myf5 null satellite cells and myoblasts, therefore excluding a role for this determination gene in adult muscle progenitors. Skeletal muscles of adult Myf5 null mice exhibit a subtle progressive myopathy. Crucially, adult Myf5 null mice exhibit perturbed muscle regeneration with a significant increase in muscle fibre hypertrophy, delayed differentiation, adipocyte accumulation, and fibrosis after freeze-injury. Satellite cell numbers are not significantly altered in Myf5 null animals and they show a modest impaired proliferation under some conditions in vitro. Mice double mutant for Myf5 and Dystrophin were more severely affected than single mutants, with enhanced necrosis and regeneration. Therefore, we show that Myf5 is a regulator of regenerative myogenesis and homeostasis, with functions distinct from those of Myod and Mrf4.

Authors+Show Affiliations

Stem Cells and Development, Dept. of Developmental Biology, Pasteur Institute, CNRS URA 2578, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17961534

Citation

Gayraud-Morel, Barbara, et al. "A Role for the Myogenic Determination Gene Myf5 in Adult Regenerative Myogenesis." Developmental Biology, vol. 312, no. 1, 2007, pp. 13-28.
Gayraud-Morel B, Chrétien F, Flamant P, et al. A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol. 2007;312(1):13-28.
Gayraud-Morel, B., Chrétien, F., Flamant, P., Gomès, D., Zammit, P. S., & Tajbakhsh, S. (2007). A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Developmental Biology, 312(1), 13-28.
Gayraud-Morel B, et al. A Role for the Myogenic Determination Gene Myf5 in Adult Regenerative Myogenesis. Dev Biol. 2007 Dec 1;312(1):13-28. PubMed PMID: 17961534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. AU - Gayraud-Morel,Barbara, AU - Chrétien,Fabrice, AU - Flamant,Patricia, AU - Gomès,Danielle, AU - Zammit,Peter S, AU - Tajbakhsh,Shahragim, Y1 - 2007/09/11/ PY - 2007/07/20/received PY - 2007/08/13/revised PY - 2007/08/13/accepted PY - 2007/10/27/pubmed PY - 2007/12/28/medline PY - 2007/10/27/entrez SP - 13 EP - 28 JF - Developmental biology JO - Dev Biol VL - 312 IS - 1 N2 - The myogenic determination genes Myf5, Myod and Mrf4 direct skeletal muscle cell fate prenatally. In adult myogenesis, Myod has been shown to regulate myoblast differentiation, however, our understanding of satellite cell regulation is incomplete since the roles of Myf5 and Mrf4 had not been clearly defined. Here we examine the function of Myf5 and Mrf4 in the adult using recently generated alleles. Mrf4 is not expressed in normal or Myf5 null satellite cells and myoblasts, therefore excluding a role for this determination gene in adult muscle progenitors. Skeletal muscles of adult Myf5 null mice exhibit a subtle progressive myopathy. Crucially, adult Myf5 null mice exhibit perturbed muscle regeneration with a significant increase in muscle fibre hypertrophy, delayed differentiation, adipocyte accumulation, and fibrosis after freeze-injury. Satellite cell numbers are not significantly altered in Myf5 null animals and they show a modest impaired proliferation under some conditions in vitro. Mice double mutant for Myf5 and Dystrophin were more severely affected than single mutants, with enhanced necrosis and regeneration. Therefore, we show that Myf5 is a regulator of regenerative myogenesis and homeostasis, with functions distinct from those of Myod and Mrf4. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/17961534/A_role_for_the_myogenic_determination_gene_Myf5_in_adult_regenerative_myogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(07)01274-2 DB - PRIME DP - Unbound Medicine ER -