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Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs.
J Physiol 2007; 585(Pt 3):881-95JP

Abstract

Cajal-Retzius (CR) cells are principal cells of layer I in the developing neocortex. They are able to generate action potentials, make synaptic contacts in layer I and receive excitatory GABAergic inputs before birth. Although CR cells participate in neuronal network activity in layer I, the properties of their synaptic inputs are not yet characterized. We recorded miniature (mIPSCs) and evoked (eIPSCs) postsynaptic currents using the whole-cell patch-clamp technique. Most of CR cells displayed two types of mIPSCs, namely those with fast (mIPSC(F)) and slow (mIPSC(S)) rise kinetics. The mIPSC(F) mean amplitude was significantly larger than that of mIPSC(S), while their decay rates were not different. Peak-scaled non-stationary noise analysis revealed that mIPSC(S) and mIPSC(F) differed in their weighted single-channel conductance. In addition, zolpidem (100 nm), a modulator of alpha(1) subunit-containing GABA(A) receptors, selectively affected mIPSC(S) suggesting that different postsynaptic GABA(A) receptors mediate mIPSC(F) and mIPSC(S). eIPSCs also split into two populations with different rise kinetics. Fast eIPSCs (eIPSC(F)) displayed higher paired-pulse ratio (PPR) and lower GABA release probability than slowly rising eIPSCs (eIPSC(S)). As CGP55845, a GABA(B) receptor antagonist, eliminated the observed difference in PPR, the lower release probability at IPSC(F) connections probably reflects a stronger tonic GABA(B) receptor-mediated inhibition of IPSC(F) synapses. At low (0.1 Hz) stimulation frequency both inputs can effectively convert presynaptic action potentials into postsynaptic ones; however, only IPSC(F) connections reliably transfer the presynaptic activity patterns at higher stimulation rates. Thus, CR cells receive two GABAergic inputs, which differ in the quantal amplitude, the probability of GABA release and the frequency dependence of signal transfer.

Authors+Show Affiliations

Institute of Neurophysiology, Johannes-Mueller-Center of Physiology, Charité-University-Medicine Berlin, Tucholskystrasse 2, 10117 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17962325

Citation

Kirmse, Knut, et al. "Cajal Retzius Cells in the Mouse Neocortex Receive Two Types of Pre- and Postsynaptically Distinct GABAergic Inputs." The Journal of Physiology, vol. 585, no. Pt 3, 2007, pp. 881-95.
Kirmse K, Dvorzhak A, Henneberger C, et al. Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs. J Physiol (Lond). 2007;585(Pt 3):881-95.
Kirmse, K., Dvorzhak, A., Henneberger, C., Grantyn, R., & Kirischuk, S. (2007). Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs. The Journal of Physiology, 585(Pt 3), pp. 881-95.
Kirmse K, et al. Cajal Retzius Cells in the Mouse Neocortex Receive Two Types of Pre- and Postsynaptically Distinct GABAergic Inputs. J Physiol (Lond). 2007 Dec 15;585(Pt 3):881-95. PubMed PMID: 17962325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs. AU - Kirmse,Knut, AU - Dvorzhak,Anton, AU - Henneberger,Christian, AU - Grantyn,Rosemarie, AU - Kirischuk,Sergei, Y1 - 2007/10/25/ PY - 2007/10/27/pubmed PY - 2008/2/26/medline PY - 2007/10/27/entrez SP - 881 EP - 95 JF - The Journal of physiology JO - J. Physiol. (Lond.) VL - 585 IS - Pt 3 N2 - Cajal-Retzius (CR) cells are principal cells of layer I in the developing neocortex. They are able to generate action potentials, make synaptic contacts in layer I and receive excitatory GABAergic inputs before birth. Although CR cells participate in neuronal network activity in layer I, the properties of their synaptic inputs are not yet characterized. We recorded miniature (mIPSCs) and evoked (eIPSCs) postsynaptic currents using the whole-cell patch-clamp technique. Most of CR cells displayed two types of mIPSCs, namely those with fast (mIPSC(F)) and slow (mIPSC(S)) rise kinetics. The mIPSC(F) mean amplitude was significantly larger than that of mIPSC(S), while their decay rates were not different. Peak-scaled non-stationary noise analysis revealed that mIPSC(S) and mIPSC(F) differed in their weighted single-channel conductance. In addition, zolpidem (100 nm), a modulator of alpha(1) subunit-containing GABA(A) receptors, selectively affected mIPSC(S) suggesting that different postsynaptic GABA(A) receptors mediate mIPSC(F) and mIPSC(S). eIPSCs also split into two populations with different rise kinetics. Fast eIPSCs (eIPSC(F)) displayed higher paired-pulse ratio (PPR) and lower GABA release probability than slowly rising eIPSCs (eIPSC(S)). As CGP55845, a GABA(B) receptor antagonist, eliminated the observed difference in PPR, the lower release probability at IPSC(F) connections probably reflects a stronger tonic GABA(B) receptor-mediated inhibition of IPSC(F) synapses. At low (0.1 Hz) stimulation frequency both inputs can effectively convert presynaptic action potentials into postsynaptic ones; however, only IPSC(F) connections reliably transfer the presynaptic activity patterns at higher stimulation rates. Thus, CR cells receive two GABAergic inputs, which differ in the quantal amplitude, the probability of GABA release and the frequency dependence of signal transfer. SN - 0022-3751 UR - https://www.unboundmedicine.com/medline/citation/17962325/Cajal_Retzius_cells_in_the_mouse_neocortex_receive_two_types_of_pre__and_postsynaptically_distinct_GABAergic_inputs_ L2 - https://doi.org/10.1113/jphysiol.2007.145003 DB - PRIME DP - Unbound Medicine ER -