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[Serological screening of relatives of celiac disease patients: antiendomysium antibodies, anti-tissue transglutaminase or both?].

Abstract

BACKGROUND

Celiac disease is the most common intestinal disorder of caucasian populations and presents a prevalence of 8% to 18% between the relatives of patients. The anti-endomysial (IgA-EmA) and anti-tissue transglutaminase antibodies (IgA-tTG) have represented an important non invasive and sensitivity method of screening and diagnosis of celiac disease in risk groups and populations.

AIM

To investigate the prevalence of IgA-EmA and IgA-tTG antibodies in relatives of celiac patients and verify the degree of concordance between them.

METHODS

One hundred and seventy seven relatives of celiac patients (76(feminino); 101(masculino); 2-79 years) and 93 healthy individuals were evaluated (34(feminino); 59(masculino); 2-71 years). IgA-EmA were detected by indirect immunofluorescence, with human umbilical cord as substrate, while anti-IgA-tTG titers were measured by enzyme-linked immunosorbent assay (ELISA), using commercial kit.

RESULTS

Total positivity to antibodies in relatives of celiac patients was of 21% (37/177), and showed significant difference compared to control group (0%; 0/93). Twelve percent (21/177) of celiac disease relatives were positive to IgA-EmA, 13.56% (24/177) to IgA-tTG, and 4.52% (8/177) to both assays simultaneously. The concordance between both methods was 83.6% (148/177) and the discordance was 16.4% (29/177), with a positive and significant correlation (r = 0.435). Among the concordant results, 79.1% (140/177) were negative and 4.52% (8/177) were positive to both antibodies. Among the discordant results, 7.34% (13/177) were positive to IgA-EmA and negative to IgA-tTG, while 9.04% (16/177) were negative to IgA- EmA and positive to IgA-tTG.

CONCLUSION

Although the high positivity to IgA-EmA and IgA-tTG emphasizes the importance of the serological screening in relatives of celiac patients, the discordances detected in this study showed that the use of only one method can lead to false negative results. Consequently these relatives will not be submitted to intestinal biopsy to confirm the diagnosis of celiac disease, and to the correct and earlier treatment.

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  • Authors+Show Affiliations

    ,

    Laboratório de Imunopatologia, Hospital de Clínicas, Universidade Federal do Paraná, Curatiba, PR. shirley@ufpr.br

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    Source

    MeSH

    Adolescent
    Adult
    Aged
    Autoantibodies
    Case-Control Studies
    Celiac Disease
    Child
    Child, Preschool
    Enzyme-Linked Immunosorbent Assay
    Female
    Fluorescent Antibody Technique, Indirect
    Humans
    Immunoglobulin A
    Male
    Middle Aged
    Transglutaminases

    Pub Type(s)

    English Abstract
    Journal Article

    Language

    por

    PubMed ID

    17962863

    Citation

    Utiyama, Shirley Ramos da Rosa, et al. "[Serological Screening of Relatives of Celiac Disease Patients: Antiendomysium Antibodies, Anti-tissue Transglutaminase or Both?]." Arquivos De Gastroenterologia, vol. 44, no. 2, 2007, pp. 156-61.
    Utiyama SR, Nass FR, Kotze LM, et al. [Serological screening of relatives of celiac disease patients: antiendomysium antibodies, anti-tissue transglutaminase or both?]. Arq Gastroenterol. 2007;44(2):156-61.
    Utiyama, S. R., Nass, F. R., Kotze, L. M., Nisihara, R. M., Ambrosio, A. R., & Messias-Reason, I. T. (2007). [Serological screening of relatives of celiac disease patients: antiendomysium antibodies, anti-tissue transglutaminase or both?]. Arquivos De Gastroenterologia, 44(2), pp. 156-61.
    Utiyama SR, et al. [Serological Screening of Relatives of Celiac Disease Patients: Antiendomysium Antibodies, Anti-tissue Transglutaminase or Both?]. Arq Gastroenterol. 2007;44(2):156-61. PubMed PMID: 17962863.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - [Serological screening of relatives of celiac disease patients: antiendomysium antibodies, anti-tissue transglutaminase or both?]. AU - Utiyama,Shirley Ramos da Rosa, AU - Nass,Flávia Raphaela, AU - Kotze,Lorete Maria da Silva, AU - Nisihara,Renato Mitsunori, AU - Ambrosio,Altair Rogério, AU - Messias-Reason,Iara Taborda de, PY - 2006/06/20/received PY - 2007/01/24/accepted PY - 2007/10/27/pubmed PY - 2008/6/11/medline PY - 2007/10/27/entrez SP - 156 EP - 61 JF - Arquivos de gastroenterologia JO - Arq Gastroenterol VL - 44 IS - 2 N2 - BACKGROUND: Celiac disease is the most common intestinal disorder of caucasian populations and presents a prevalence of 8% to 18% between the relatives of patients. The anti-endomysial (IgA-EmA) and anti-tissue transglutaminase antibodies (IgA-tTG) have represented an important non invasive and sensitivity method of screening and diagnosis of celiac disease in risk groups and populations. AIM: To investigate the prevalence of IgA-EmA and IgA-tTG antibodies in relatives of celiac patients and verify the degree of concordance between them. METHODS: One hundred and seventy seven relatives of celiac patients (76(feminino); 101(masculino); 2-79 years) and 93 healthy individuals were evaluated (34(feminino); 59(masculino); 2-71 years). IgA-EmA were detected by indirect immunofluorescence, with human umbilical cord as substrate, while anti-IgA-tTG titers were measured by enzyme-linked immunosorbent assay (ELISA), using commercial kit. RESULTS: Total positivity to antibodies in relatives of celiac patients was of 21% (37/177), and showed significant difference compared to control group (0%; 0/93). Twelve percent (21/177) of celiac disease relatives were positive to IgA-EmA, 13.56% (24/177) to IgA-tTG, and 4.52% (8/177) to both assays simultaneously. The concordance between both methods was 83.6% (148/177) and the discordance was 16.4% (29/177), with a positive and significant correlation (r = 0.435). Among the concordant results, 79.1% (140/177) were negative and 4.52% (8/177) were positive to both antibodies. Among the discordant results, 7.34% (13/177) were positive to IgA-EmA and negative to IgA-tTG, while 9.04% (16/177) were negative to IgA- EmA and positive to IgA-tTG. CONCLUSION: Although the high positivity to IgA-EmA and IgA-tTG emphasizes the importance of the serological screening in relatives of celiac patients, the discordances detected in this study showed that the use of only one method can lead to false negative results. Consequently these relatives will not be submitted to intestinal biopsy to confirm the diagnosis of celiac disease, and to the correct and earlier treatment. SN - 0004-2803 UR - https://www.unboundmedicine.com/medline/citation/17962863/[Serological_screening_of_relatives_of_celiac_disease_patients:_antiendomysium_antibodies_anti_tissue_transglutaminase_or_both]_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032007000200014&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -