Tags

Type your tag names separated by a space and hit enter

Chemical screening of olive biophenol extracts by hyphenated liquid chromatography.
Anal Chim Acta. 2007 Nov 12; 603(2):176-89.AC

Abstract

Chemical screening using reversed phase HPLC-photodiode array detection (RPLC-DAD) and RPLC-electrospray ionisation mass spectrometry (RPLC-ESI-MS) is widely applied as an approach to streamline natural products research. The full potential of this approach is demonstrated in this paper by application to the chemical screening of olive products including olive mill waste (OMW). Out of 100 biophenols previously reported in olive products, the on-line RPLC-DAD-ESI-MS was able to confirm the presence of 52 compounds in OMW. This included a number of simple phenols, flavonoids and secoiridoids. By careful examination of the combined DAD and ESI-MS data, extra information was elucidated including: the site of glycosidation on the phenol ring of hydroxytyrosol; the identity of the other luteolin-glucoside isomer as luteolin-4'-O-glucoside; identifying rutin rather than the previously reported hesperidin (and the reasons for possible mis-assignment); and the detection of diastereomers of 4-hydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (4-HPEA-DEDA) and 3,4-dihydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (3,4-DHPEA-DEDA).

Links

Publisher Full Text

Authors+Show Affiliations

Obied HK
E.H. Graham Centre for Agricultural Innovation, School of Wine and Food Science, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
Bedgood DR
No affiliation info available
Prenzler PD
No affiliation info available
Robards K
No affiliation info available

MeSH

ChromansChromatography, High Pressure LiquidFlavonoidsIridoidsLignansOleaOlive OilPhenolsPlant OilsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17963838

Citation

Obied, Hassan K., et al. "Chemical Screening of Olive Biophenol Extracts By Hyphenated Liquid Chromatography." Analytica Chimica Acta, vol. 603, no. 2, 2007, pp. 176-89.
Obied HK, Bedgood DR, Prenzler PD, et al. Chemical screening of olive biophenol extracts by hyphenated liquid chromatography. Anal Chim Acta. 2007;603(2):176-89.
Obied, H. K., Bedgood, D. R., Prenzler, P. D., & Robards, K. (2007). Chemical screening of olive biophenol extracts by hyphenated liquid chromatography. Analytica Chimica Acta, 603(2), 176-89.
Obied HK, et al. Chemical Screening of Olive Biophenol Extracts By Hyphenated Liquid Chromatography. Anal Chim Acta. 2007 Nov 12;603(2):176-89. PubMed PMID: 17963838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical screening of olive biophenol extracts by hyphenated liquid chromatography. AU - Obied,Hassan K, AU - Bedgood,Danny R,Jr AU - Prenzler,Paul D, AU - Robards,Kevin, Y1 - 2007/09/26/ PY - 2007/08/20/received PY - 2007/09/20/revised PY - 2007/09/20/accepted PY - 2007/10/30/pubmed PY - 2007/11/9/medline PY - 2007/10/30/entrez SP - 176 EP - 89 JF - Analytica chimica acta JO - Anal Chim Acta VL - 603 IS - 2 N2 - Chemical screening using reversed phase HPLC-photodiode array detection (RPLC-DAD) and RPLC-electrospray ionisation mass spectrometry (RPLC-ESI-MS) is widely applied as an approach to streamline natural products research. The full potential of this approach is demonstrated in this paper by application to the chemical screening of olive products including olive mill waste (OMW). Out of 100 biophenols previously reported in olive products, the on-line RPLC-DAD-ESI-MS was able to confirm the presence of 52 compounds in OMW. This included a number of simple phenols, flavonoids and secoiridoids. By careful examination of the combined DAD and ESI-MS data, extra information was elucidated including: the site of glycosidation on the phenol ring of hydroxytyrosol; the identity of the other luteolin-glucoside isomer as luteolin-4'-O-glucoside; identifying rutin rather than the previously reported hesperidin (and the reasons for possible mis-assignment); and the detection of diastereomers of 4-hydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (4-HPEA-DEDA) and 3,4-dihydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (3,4-DHPEA-DEDA). SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17963838/Chemical_screening_of_olive_biophenol_extracts_by_hyphenated_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)01580-2 DB - PRIME DP - Unbound Medicine ER -