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Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry.
J Chromatogr A. 2007 Nov 30; 1173(1-2):81-7.JC

Abstract

This paper discusses the requirement for, and presents an analytical procedure for, the determination of four steroid hormones and a conjugated steroid (estrone-3-sulfate) in wastewaters. The method utilizes LC/MS/MS following solid phase extraction and a two stage clean-up procedure, achieving limits of detection of 0.2 ng l(-1) for estriol, 17beta-estradiol and 17 alpha-ethinylestradiol, and 0.1 ng l(-1) for estrone and the conjugate. The approach demonstrates that using appropriate clean-up and deuterated internal standards, the impact of matrix effects on ionization can be overcome to reliably determine estrogens at environmentally relevant concentrations. The robustness of the method was demonstrated by achieving recoveries of >83% for all steroids in settled sewage and final effluent samples with relative standard deviations of 0.5-12%.

Authors+Show Affiliations

Faculty of Medicine, Division of Experimental Medicine and Toxicology, Imperial College London, Hammersmith Campus, London W12 ONN, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17964588

Citation

Koh, Y K K., et al. "Determination of Steroid Estrogens in Wastewater By High Performance Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1173, no. 1-2, 2007, pp. 81-7.
Koh YK, Chiu TY, Boobis A, et al. Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2007;1173(1-2):81-7.
Koh, Y. K., Chiu, T. Y., Boobis, A., Cartmell, E., Lester, J. N., & Scrimshaw, M. D. (2007). Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1173(1-2), 81-7.
Koh YK, et al. Determination of Steroid Estrogens in Wastewater By High Performance Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2007 Nov 30;1173(1-2):81-7. PubMed PMID: 17964588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry. AU - Koh,Y K K, AU - Chiu,T Y, AU - Boobis,A, AU - Cartmell,E, AU - Lester,J N, AU - Scrimshaw,M D, Y1 - 2007/10/10/ PY - 2007/05/03/received PY - 2007/08/07/revised PY - 2007/09/30/accepted PY - 2007/10/30/pubmed PY - 2008/1/9/medline PY - 2007/10/30/entrez SP - 81 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1173 IS - 1-2 N2 - This paper discusses the requirement for, and presents an analytical procedure for, the determination of four steroid hormones and a conjugated steroid (estrone-3-sulfate) in wastewaters. The method utilizes LC/MS/MS following solid phase extraction and a two stage clean-up procedure, achieving limits of detection of 0.2 ng l(-1) for estriol, 17beta-estradiol and 17 alpha-ethinylestradiol, and 0.1 ng l(-1) for estrone and the conjugate. The approach demonstrates that using appropriate clean-up and deuterated internal standards, the impact of matrix effects on ionization can be overcome to reliably determine estrogens at environmentally relevant concentrations. The robustness of the method was demonstrated by achieving recoveries of >83% for all steroids in settled sewage and final effluent samples with relative standard deviations of 0.5-12%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17964588/Determination_of_steroid_estrogens_in_wastewater_by_high_performance_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)01704-9 DB - PRIME DP - Unbound Medicine ER -