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Direct NMR analysis of cannabis water extracts and tinctures and semi-quantitative data on delta9-THC and delta9-THC-acid.
Phytochemistry. 2008 Jan; 69(2):562-70.P

Abstract

Cannabis sativa L. is the source for a whole series of chemically diverse bioactive compounds that are currently under intensive pharmaceutical investigation. In this work, hot and cold water extracts as well as ethanol/water mixtures (tinctures) of cannabis were compared in order to better understand how these extracts differ in their overall composition. NMR analysis and in vitro cell assays of crude extracts and fractions were performed. Manufacturing procedures to produce natural remedies can strongly affect the final composition of the herbal medicines. Temperature and polarity of the solvents used for the extraction resulted to be two factors that affect the total amount of Delta(9)-THC in the extracts and its relative quantity with respect to Delta(9)-THC-acid and other metabolites. Diffusion-edited (1)H NMR (1D DOSY) and (1)H NMR with suppression of the ethanol and water signals were used. With this method it was possible, without any evaporation or separation step, to distinguish between tinctures from different cannabis cultivars. This approach is proposed as a direct analysis of plant tinctures.

Authors+Show Affiliations

Centre for Pharmacognosy and Phytotherapy, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17964620

Citation

Politi, M, et al. "Direct NMR Analysis of Cannabis Water Extracts and Tinctures and Semi-quantitative Data On delta9-THC and Delta9-THC-acid." Phytochemistry, vol. 69, no. 2, 2008, pp. 562-70.
Politi M, Peschel W, Wilson N, et al. Direct NMR analysis of cannabis water extracts and tinctures and semi-quantitative data on delta9-THC and delta9-THC-acid. Phytochemistry. 2008;69(2):562-70.
Politi, M., Peschel, W., Wilson, N., Zloh, M., Prieto, J. M., & Heinrich, M. (2008). Direct NMR analysis of cannabis water extracts and tinctures and semi-quantitative data on delta9-THC and delta9-THC-acid. Phytochemistry, 69(2), 562-70.
Politi M, et al. Direct NMR Analysis of Cannabis Water Extracts and Tinctures and Semi-quantitative Data On delta9-THC and Delta9-THC-acid. Phytochemistry. 2008;69(2):562-70. PubMed PMID: 17964620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct NMR analysis of cannabis water extracts and tinctures and semi-quantitative data on delta9-THC and delta9-THC-acid. AU - Politi,M, AU - Peschel,W, AU - Wilson,N, AU - Zloh,M, AU - Prieto,J M, AU - Heinrich,M, Y1 - 2007/10/26/ PY - 2007/02/07/received PY - 2007/07/21/revised PY - 2007/07/25/accepted PY - 2007/10/30/pubmed PY - 2008/5/8/medline PY - 2007/10/30/entrez SP - 562 EP - 70 JF - Phytochemistry JO - Phytochemistry VL - 69 IS - 2 N2 - Cannabis sativa L. is the source for a whole series of chemically diverse bioactive compounds that are currently under intensive pharmaceutical investigation. In this work, hot and cold water extracts as well as ethanol/water mixtures (tinctures) of cannabis were compared in order to better understand how these extracts differ in their overall composition. NMR analysis and in vitro cell assays of crude extracts and fractions were performed. Manufacturing procedures to produce natural remedies can strongly affect the final composition of the herbal medicines. Temperature and polarity of the solvents used for the extraction resulted to be two factors that affect the total amount of Delta(9)-THC in the extracts and its relative quantity with respect to Delta(9)-THC-acid and other metabolites. Diffusion-edited (1)H NMR (1D DOSY) and (1)H NMR with suppression of the ethanol and water signals were used. With this method it was possible, without any evaporation or separation step, to distinguish between tinctures from different cannabis cultivars. This approach is proposed as a direct analysis of plant tinctures. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/17964620/Direct_NMR_analysis_of_cannabis_water_extracts_and_tinctures_and_semi_quantitative_data_on_delta9_THC_and_delta9_THC_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(07)00495-5 DB - PRIME DP - Unbound Medicine ER -