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Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.
Bioresour Technol. 2008 Jul; 99(11):4896-902.BT

Abstract

Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. The optimized conditions for the extraction from core resulted in forward and back extraction efficiencies of 45% and 62%, respectively, using reverse micellar system of cationic surfactant CTAB. A fairly good activity recovery (106%) and purification (5.2-fold) of bromelain is obtained under these conditions. Reverse micellar extraction from peel, extended stem and crown using CTAB system resulted in purification folds of 2.1, 3.5, and 1.7, respectively. Extraction from extended stem using anionic surfactant AOT in isooctane did not yield good results under the operating conditions employed.

Authors+Show Affiliations

Department of Food Engineering, Central Food Technological Research Institute, Mysore 570 020, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17964777

Citation

Umesh Hebbar, H, et al. "Use of Reverse Micellar Systems for the Extraction and Purification of Bromelain From Pineapple Wastes." Bioresource Technology, vol. 99, no. 11, 2008, pp. 4896-902.
Umesh Hebbar H, Sumana B, Raghavarao KS. Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresour Technol. 2008;99(11):4896-902.
Umesh Hebbar, H., Sumana, B., & Raghavarao, K. S. (2008). Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresource Technology, 99(11), 4896-902.
Umesh Hebbar H, Sumana B, Raghavarao KS. Use of Reverse Micellar Systems for the Extraction and Purification of Bromelain From Pineapple Wastes. Bioresour Technol. 2008;99(11):4896-902. PubMed PMID: 17964777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. AU - Umesh Hebbar,H, AU - Sumana,B, AU - Raghavarao,K S M S, Y1 - 2007/10/26/ PY - 2007/04/20/received PY - 2007/09/11/revised PY - 2007/09/15/accepted PY - 2007/10/30/pubmed PY - 2008/8/5/medline PY - 2007/10/30/entrez SP - 4896 EP - 902 JF - Bioresource technology JO - Bioresour. Technol. VL - 99 IS - 11 N2 - Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. The optimized conditions for the extraction from core resulted in forward and back extraction efficiencies of 45% and 62%, respectively, using reverse micellar system of cationic surfactant CTAB. A fairly good activity recovery (106%) and purification (5.2-fold) of bromelain is obtained under these conditions. Reverse micellar extraction from peel, extended stem and crown using CTAB system resulted in purification folds of 2.1, 3.5, and 1.7, respectively. Extraction from extended stem using anionic surfactant AOT in isooctane did not yield good results under the operating conditions employed. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/17964777/Use_of_reverse_micellar_systems_for_the_extraction_and_purification_of_bromelain_from_pineapple_wastes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(07)00784-5 DB - PRIME DP - Unbound Medicine ER -