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Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa.
J Antimicrob Chemother. 2008 Jan; 61(1):117-22.JA

Abstract

OBJECTIVES

Lucilia sericata maggots are successfully used for treating chronic wounds. As the healing process in these wounds is complicated by bacteria, particularly when residing in biofilms that protect them from antibiotics and the immune system, we assessed the effects of maggot excretions/secretions (ES) on Staphylococcus aureus and Pseudomonas aeruginosa biofilms, the clinically most relevant species.

METHODS

We assessed the effects of ES on biofilms using microtitre plate assays, on bacterial viability using in vitro killing and radial diffusion assays, and on quorum sensing systems using specific reporter bacteria.

RESULTS

As little as 0.2 microg of ES prevented S. aureus biofilm formation and 2 microg of ES rapidly degraded biofilms. In contrast, ES initially promoted P. aeruginosa biofilm formation, but after 10 h the biofilms collapsed. Degradation of P. aeruginosa biofilms started after 10 h and required 10-fold more ES than S. aureus biofilms. Boiling of ES abrogated their effects on S. aureus, but not on P. aeruginosa, biofilms, indicating that different molecules within ES are responsible for the observed effects. Modulation of biofilms by ES did not involve bacterial killing or effects on quorum sensing systems.

CONCLUSIONS

Maggot ES are differentially effective against biofilms of S. aureus and P. aeruginosa.

Links

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Authors+Show Affiliations

van der Plas MJ
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
Jukema GN
No affiliation info available
Wai SW
No affiliation info available
Dogterom-Ballering HC
No affiliation info available
Lagendijk EL
No affiliation info available
van Gulpen C
No affiliation info available
van Dissel JT
No affiliation info available
Bloemberg GV
No affiliation info available
Nibbering PH
No affiliation info available

MeSH

AnimalsAnti-Bacterial AgentsBiofilmsDipteraLarvaPseudomonas aeruginosaStaphylococcusWound Healing

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17965032

Citation

van der Plas, Mariena J A., et al. "Maggot Excretions/secretions Are Differentially Effective Against Biofilms of Staphylococcus Aureus and Pseudomonas Aeruginosa." The Journal of Antimicrobial Chemotherapy, vol. 61, no. 1, 2008, pp. 117-22.
van der Plas MJ, Jukema GN, Wai SW, et al. Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. J Antimicrob Chemother. 2008;61(1):117-22.
van der Plas, M. J., Jukema, G. N., Wai, S. W., Dogterom-Ballering, H. C., Lagendijk, E. L., van Gulpen, C., van Dissel, J. T., Bloemberg, G. V., & Nibbering, P. H. (2008). Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. The Journal of Antimicrobial Chemotherapy, 61(1), 117-22.
van der Plas MJ, et al. Maggot Excretions/secretions Are Differentially Effective Against Biofilms of Staphylococcus Aureus and Pseudomonas Aeruginosa. J Antimicrob Chemother. 2008;61(1):117-22. PubMed PMID: 17965032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. AU - van der Plas,Mariena J A, AU - Jukema,Gerrolt N, AU - Wai,Sin-Wen, AU - Dogterom-Ballering,Heleen C M, AU - Lagendijk,Ellen L, AU - van Gulpen,Co, AU - van Dissel,Jaap T, AU - Bloemberg,Guido V, AU - Nibbering,Peter H, Y1 - 2007/10/26/ PY - 2007/10/30/pubmed PY - 2008/2/29/medline PY - 2007/10/30/entrez SP - 117 EP - 22 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 61 IS - 1 N2 - OBJECTIVES: Lucilia sericata maggots are successfully used for treating chronic wounds. As the healing process in these wounds is complicated by bacteria, particularly when residing in biofilms that protect them from antibiotics and the immune system, we assessed the effects of maggot excretions/secretions (ES) on Staphylococcus aureus and Pseudomonas aeruginosa biofilms, the clinically most relevant species. METHODS: We assessed the effects of ES on biofilms using microtitre plate assays, on bacterial viability using in vitro killing and radial diffusion assays, and on quorum sensing systems using specific reporter bacteria. RESULTS: As little as 0.2 microg of ES prevented S. aureus biofilm formation and 2 microg of ES rapidly degraded biofilms. In contrast, ES initially promoted P. aeruginosa biofilm formation, but after 10 h the biofilms collapsed. Degradation of P. aeruginosa biofilms started after 10 h and required 10-fold more ES than S. aureus biofilms. Boiling of ES abrogated their effects on S. aureus, but not on P. aeruginosa, biofilms, indicating that different molecules within ES are responsible for the observed effects. Modulation of biofilms by ES did not involve bacterial killing or effects on quorum sensing systems. CONCLUSIONS: Maggot ES are differentially effective against biofilms of S. aureus and P. aeruginosa. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/17965032/Maggot_excretions/secretions_are_differentially_effective_against_biofilms_of_Staphylococcus_aureus_and_Pseudomonas_aeruginosa_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkm407 DB - PRIME DP - Unbound Medicine ER -