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Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia.
Adv Exp Med Biol. 2007; 603:156-66.AE

Abstract

During infections of the intestinal tract Yersinia pseudotuberculosis penetrates the epithelial cell layer through M-cells into the Peyer's patches. This early step in the infection process is primarily mediated by the outer membrane protein invasin. Expression of the invasin gene is activated by the MarR-type regulatory protein RovA in response to environmental conditions, including temperature and growth phase. In order to gain insight into the nature of the underlying control systems, mutagenesis and gene bank screens were used to identify regula components modulating the levels of invasin and RovA. We found that the inv and rovA genes were both subjected to silencing by the nucleoid-associated protein H-NS. Under inducing conditions, RovA appears to disrupt the silencer complex, through displacement of H-NS from an extended AT-rich region located upstream of the inv and rovA promoters. Furthermore, a LysR-type regulatory protein, RovM with homology to HexA/PecT of phytopathogenic Erwinia species was shown to interact specifically with the rovA regulatory region and represses rovA transcription in addition to H-NS. Disruption of the rovM gene significantly enhanced internalization of Y. pseudotuberculosis into host cells and higher numbers of the mutant bacteria were detectable in gut-associated lymphatic tissues and organs in infected mice. In addition, the histone-like protein YmoA, which has a global effect on the bacterial physiology, was found to activate rovA expression through RovM. Together, our studies showed, that H-NS, RovM and YmoA are key regulators implicated in the environmental control of virulence factors, which are important for the initiation of a Yersinia infection.

Authors+Show Affiliations

Institut für Mikrobiologie, Technische Universität Braunschweig, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

17966412

Citation

Heroven, Ann Kathrin, et al. "Regulatory Elements Implicated in the Environmental Control of Invasin Expression in Enteropathogenic Yersinia." Advances in Experimental Medicine and Biology, vol. 603, 2007, pp. 156-66.
Heroven AK, Böhme K, Tran-Winkler H, et al. Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia. Adv Exp Med Biol. 2007;603:156-66.
Heroven, A. K., Böhme, K., Tran-Winkler, H., & Dersch, P. (2007). Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia. Advances in Experimental Medicine and Biology, 603, 156-66.
Heroven AK, et al. Regulatory Elements Implicated in the Environmental Control of Invasin Expression in Enteropathogenic Yersinia. Adv Exp Med Biol. 2007;603:156-66. PubMed PMID: 17966412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia. AU - Heroven,Ann Kathrin, AU - Böhme,Katja, AU - Tran-Winkler,Hien, AU - Dersch,Petra, PY - 2007/10/31/pubmed PY - 2007/12/8/medline PY - 2007/10/31/entrez SP - 156 EP - 66 JF - Advances in experimental medicine and biology JO - Adv Exp Med Biol VL - 603 N2 - During infections of the intestinal tract Yersinia pseudotuberculosis penetrates the epithelial cell layer through M-cells into the Peyer's patches. This early step in the infection process is primarily mediated by the outer membrane protein invasin. Expression of the invasin gene is activated by the MarR-type regulatory protein RovA in response to environmental conditions, including temperature and growth phase. In order to gain insight into the nature of the underlying control systems, mutagenesis and gene bank screens were used to identify regula components modulating the levels of invasin and RovA. We found that the inv and rovA genes were both subjected to silencing by the nucleoid-associated protein H-NS. Under inducing conditions, RovA appears to disrupt the silencer complex, through displacement of H-NS from an extended AT-rich region located upstream of the inv and rovA promoters. Furthermore, a LysR-type regulatory protein, RovM with homology to HexA/PecT of phytopathogenic Erwinia species was shown to interact specifically with the rovA regulatory region and represses rovA transcription in addition to H-NS. Disruption of the rovM gene significantly enhanced internalization of Y. pseudotuberculosis into host cells and higher numbers of the mutant bacteria were detectable in gut-associated lymphatic tissues and organs in infected mice. In addition, the histone-like protein YmoA, which has a global effect on the bacterial physiology, was found to activate rovA expression through RovM. Together, our studies showed, that H-NS, RovM and YmoA are key regulators implicated in the environmental control of virulence factors, which are important for the initiation of a Yersinia infection. SN - 0065-2598 UR - https://www.unboundmedicine.com/medline/citation/17966412/Regulatory_elements_implicated_in_the_environmental_control_of_invasin_expression_in_enteropathogenic_Yersinia_ L2 - https://dx.doi.org/10.1007/978-0-387-72124-8_13 DB - PRIME DP - Unbound Medicine ER -