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Correlation between lymph node metastasis and the expression of VEGF-C, VEGF-D and VEGFR-3 in T1 lung adenocarcinoma.
Anticancer Res. 2007 Nov-Dec; 27(6A):3735-41.AR

Abstract

Vascular endothelial growth factor (VEGF)-C and VEGF-D influence lymphangiogenesis through the activation of the vascular endothelial growth factor receptor (VEGFR)-3. They have been implicated in lymphatic tumor spread, which is an important prognostic factor in patients with non-small cell lung carcinoma (NSCLC). Whether or not the expression of VEGF-C, -D, and VEGFR-3 correlates with clinicopathological factors in patients with T1 lung adenocarcinoma was analysed. The tumor specimens were homogenized to determine the protein expression of VEGF-C, -D, and VEGFR-3 by enzyme-linked immunosorbent assay (ELISA). RNA fractions extracted from the tumor tissues were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR) to assess the mRNA levels of VEGF-C, -D, and VEGFR-3. The expression of VEGF-D protein and mRNA levels in patients without lymph node metastasis were significantly higher than those with metastasis (p=0.013, p=0.0494, respectively). However, the protein and mRNA levels of VEGF-C and VEGFR-3 were not significantly different in patients with or without metastasis. The 5-year survival rates of the patients with high VEGF-D levels were significantly higher than those of patients with low levels (p =0.0221). No significant difference in the survival rates was observed for VEGF-C and VEGFR-3. VEGF-D may be downregulated in NSCLC tissues in comparison to adjacent normal tissue, resulting in lymph node metastasis and poor prognosis.

Authors+Show Affiliations

Departments of Thoracic Surgery, Faculty of Medicine, Fukuoka University, Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan. nwnkr090@ybb.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17970036

Citation

Maekawa, Shinichi, et al. "Correlation Between Lymph Node Metastasis and the Expression of VEGF-C, VEGF-D and VEGFR-3 in T1 Lung Adenocarcinoma." Anticancer Research, vol. 27, no. 6A, 2007, pp. 3735-41.
Maekawa S, Iwasaki A, Shirakusa T, et al. Correlation between lymph node metastasis and the expression of VEGF-C, VEGF-D and VEGFR-3 in T1 lung adenocarcinoma. Anticancer Res. 2007;27(6A):3735-41.
Maekawa, S., Iwasaki, A., Shirakusa, T., Enatsu, S., Kawakami, T., Kuroki, M., & Kuroki, M. (2007). Correlation between lymph node metastasis and the expression of VEGF-C, VEGF-D and VEGFR-3 in T1 lung adenocarcinoma. Anticancer Research, 27(6A), 3735-41.
Maekawa S, et al. Correlation Between Lymph Node Metastasis and the Expression of VEGF-C, VEGF-D and VEGFR-3 in T1 Lung Adenocarcinoma. Anticancer Res. 2007 Nov-Dec;27(6A):3735-41. PubMed PMID: 17970036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between lymph node metastasis and the expression of VEGF-C, VEGF-D and VEGFR-3 in T1 lung adenocarcinoma. AU - Maekawa,Shinichi, AU - Iwasaki,Akinori, AU - Shirakusa,Takayuki, AU - Enatsu,Sotaro, AU - Kawakami,Takehito, AU - Kuroki,Motomu, AU - Kuroki,Masahide, PY - 2007/11/1/pubmed PY - 2007/12/14/medline PY - 2007/11/1/entrez SP - 3735 EP - 41 JF - Anticancer research JO - Anticancer Res VL - 27 IS - 6A N2 - Vascular endothelial growth factor (VEGF)-C and VEGF-D influence lymphangiogenesis through the activation of the vascular endothelial growth factor receptor (VEGFR)-3. They have been implicated in lymphatic tumor spread, which is an important prognostic factor in patients with non-small cell lung carcinoma (NSCLC). Whether or not the expression of VEGF-C, -D, and VEGFR-3 correlates with clinicopathological factors in patients with T1 lung adenocarcinoma was analysed. The tumor specimens were homogenized to determine the protein expression of VEGF-C, -D, and VEGFR-3 by enzyme-linked immunosorbent assay (ELISA). RNA fractions extracted from the tumor tissues were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR) to assess the mRNA levels of VEGF-C, -D, and VEGFR-3. The expression of VEGF-D protein and mRNA levels in patients without lymph node metastasis were significantly higher than those with metastasis (p=0.013, p=0.0494, respectively). However, the protein and mRNA levels of VEGF-C and VEGFR-3 were not significantly different in patients with or without metastasis. The 5-year survival rates of the patients with high VEGF-D levels were significantly higher than those of patients with low levels (p =0.0221). No significant difference in the survival rates was observed for VEGF-C and VEGFR-3. VEGF-D may be downregulated in NSCLC tissues in comparison to adjacent normal tissue, resulting in lymph node metastasis and poor prognosis. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/17970036/Correlation_between_lymph_node_metastasis_and_the_expression_of_VEGF_C_VEGF_D_and_VEGFR_3_in_T1_lung_adenocarcinoma_ DB - PRIME DP - Unbound Medicine ER -