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Uptake, transport and transformation of arsenate in radishes (Raphanus sativus).
Sci Total Environ. 2008 Feb 01; 390(1):188-97.ST

Abstract

The localization and identification of arsenic compounds in terrestrial plants are important for the understanding of arsenic uptake, transformation and translocation within these organisms, and contributes to our understanding of arsenic cycling in the environment. High performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS), and X-ray absorption near-edge structure (XANES) analysis identified arsenite, arsenate and arsenic(III)-sulphur compounds in leaf, stem and root sections of Rhaphanus sativus (radish) plants grown in both arsenic contaminated mine waste, and arsenic amended liquid cultures. The total arsenic distribution was similar between the plants grown in mine waste and those grown hydroponically. Arsenate was the predominant form of arsenic available in the growth mediums, and after it was taken up by roots, X-ray absorption spectroscopy (XAS) imaging indicated that some of the arsenate was transported to the shoots via the xylem. Additionally, arsenate was reduced by the plant and arsenic(III)-sulphur compound(s) accounted for the majority of arsenic in the leaf and stem of living plants. In this study the application of synchrotron techniques permitted the identification of arsenic(III)-sulphur species which were "invisible" to conventional HPLC-ICP-MS analysis.

Authors+Show Affiliations

Smith PG
Environmental Sciences Group, Royal Military College, P.O. Box 17000 Station Forces, Kingston, ON, Canada K7K7B4.
Koch I
No affiliation info available
Reimer KJ
No affiliation info available

MeSH

ArsenicalsBiological TransportBiotransformationPlant LeavesPlant RootsPlant StemsRaphanusSoil PollutantsSulfur Compounds

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17976691

Citation

Smith, Paula G., et al. "Uptake, Transport and Transformation of Arsenate in Radishes (Raphanus Sativus)." The Science of the Total Environment, vol. 390, no. 1, 2008, pp. 188-97.
Smith PG, Koch I, Reimer KJ. Uptake, transport and transformation of arsenate in radishes (Raphanus sativus). Sci Total Environ. 2008;390(1):188-97.
Smith, P. G., Koch, I., & Reimer, K. J. (2008). Uptake, transport and transformation of arsenate in radishes (Raphanus sativus). The Science of the Total Environment, 390(1), 188-97.
Smith PG, Koch I, Reimer KJ. Uptake, Transport and Transformation of Arsenate in Radishes (Raphanus Sativus). Sci Total Environ. 2008 Feb 1;390(1):188-97. PubMed PMID: 17976691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uptake, transport and transformation of arsenate in radishes (Raphanus sativus). AU - Smith,Paula G, AU - Koch,Iris, AU - Reimer,Kenneth J, Y1 - 2007/10/31/ PY - 2007/05/01/received PY - 2007/07/11/revised PY - 2007/09/15/accepted PY - 2007/11/3/pubmed PY - 2008/2/27/medline PY - 2007/11/3/entrez SP - 188 EP - 97 JF - The Science of the total environment JO - Sci Total Environ VL - 390 IS - 1 N2 - The localization and identification of arsenic compounds in terrestrial plants are important for the understanding of arsenic uptake, transformation and translocation within these organisms, and contributes to our understanding of arsenic cycling in the environment. High performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS), and X-ray absorption near-edge structure (XANES) analysis identified arsenite, arsenate and arsenic(III)-sulphur compounds in leaf, stem and root sections of Rhaphanus sativus (radish) plants grown in both arsenic contaminated mine waste, and arsenic amended liquid cultures. The total arsenic distribution was similar between the plants grown in mine waste and those grown hydroponically. Arsenate was the predominant form of arsenic available in the growth mediums, and after it was taken up by roots, X-ray absorption spectroscopy (XAS) imaging indicated that some of the arsenate was transported to the shoots via the xylem. Additionally, arsenate was reduced by the plant and arsenic(III)-sulphur compound(s) accounted for the majority of arsenic in the leaf and stem of living plants. In this study the application of synchrotron techniques permitted the identification of arsenic(III)-sulphur species which were "invisible" to conventional HPLC-ICP-MS analysis. SN - 0048-9697 UR - https://www.unboundmedicine.com/medline/citation/17976691/Uptake_transport_and_transformation_of_arsenate_in_radishes__Raphanus_sativus__ DB - PRIME DP - Unbound Medicine ER -