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Proinflammatory and immunoregulatory cytokines in the middle ear effusions.
Int J Pediatr Otorhinolaryngol. 2008 Jan; 72(1):13-7.IJ

Abstract

OBJECTIVE

The recognition of inflammatory mediators in middle ear effusions and their correlation with clinical parameters may allow better understanding of many complex events leading to development of permanent sequelae of otitis media and hopefully help to develop future interventions. The aim of the study was to evaluate the presence and level of proinflammatory interleukin (IL) 1beta, IL-6 and immunoregulatory IL-10 in the middle ear effusions, their mutual correlation and relationship with age, duration of the illness, number of episodes of acute otitis media, and presence of retraction pockets.

METHODS

The study included 25 children (41 ears), who had been scheduled for myringotomy with the insertion of tympanostomy tubes due to otitis media with effusion. The interview (duration of the illness, number of episodes of acute otitis media), clinical and audiological examination was conducted according to the developed examination check list. Middle ear effusions (MEE) were collected aseptically, the samples centrifuged and supernatant frozen at a temperature of -80 degrees C. The IL-1beta, IL-6 and IL-10 were assayed using enzyme-linked immunosorbent assay (ELISA) kits incorporating monoclonal antibodies and the ETI system reader. The nonparametric Mann-Whitney U test was used for statistical analysis and Pearson's linear correlation coefficient was calculated.

RESULTS

IL-1beta was detected in 80% of examined effusions, IL-6 in 78%, and IL-10 in 88%. There was a strong statistical correlation between IL-1beta and IL-6 concentrations (r=0.627, P=0.000), as well as IL-6 and IL-10 (r=0.66, P=0.000) No statistically significant correlation was found between levels of cytokines and clinical parameters.

CONCLUSIONS

Proinflammatory and immunoregulatory cytokines participate in middle ear inflammatory response. The lack of direct correlation between IL-1beta and IL-10 may be responsible for chronic character of the disease. As no correlation between the levels of cytokines and clinical parameters was found it seems that the time of duration of inflammation rather than its intensity measured by levels of cytokines is the main factor in development of middle ear mucosa pathology.

Authors+Show Affiliations

Department of Pediatric Otolaryngology, Medical Academy of Białystok, Poland. pedorl@amb.edu.plNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17976828

Citation

Skotnicka, Bozena, and Elzbieta Hassmann. "Proinflammatory and Immunoregulatory Cytokines in the Middle Ear Effusions." International Journal of Pediatric Otorhinolaryngology, vol. 72, no. 1, 2008, pp. 13-7.
Skotnicka B, Hassmann E. Proinflammatory and immunoregulatory cytokines in the middle ear effusions. Int J Pediatr Otorhinolaryngol. 2008;72(1):13-7.
Skotnicka, B., & Hassmann, E. (2008). Proinflammatory and immunoregulatory cytokines in the middle ear effusions. International Journal of Pediatric Otorhinolaryngology, 72(1), 13-7.
Skotnicka B, Hassmann E. Proinflammatory and Immunoregulatory Cytokines in the Middle Ear Effusions. Int J Pediatr Otorhinolaryngol. 2008;72(1):13-7. PubMed PMID: 17976828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proinflammatory and immunoregulatory cytokines in the middle ear effusions. AU - Skotnicka,Bozena, AU - Hassmann,Elzbieta, Y1 - 2007/10/31/ PY - 2007/08/02/received PY - 2007/09/03/revised PY - 2007/09/05/accepted PY - 2007/11/3/pubmed PY - 2008/4/23/medline PY - 2007/11/3/entrez SP - 13 EP - 7 JF - International journal of pediatric otorhinolaryngology JO - Int J Pediatr Otorhinolaryngol VL - 72 IS - 1 N2 - OBJECTIVE: The recognition of inflammatory mediators in middle ear effusions and their correlation with clinical parameters may allow better understanding of many complex events leading to development of permanent sequelae of otitis media and hopefully help to develop future interventions. The aim of the study was to evaluate the presence and level of proinflammatory interleukin (IL) 1beta, IL-6 and immunoregulatory IL-10 in the middle ear effusions, their mutual correlation and relationship with age, duration of the illness, number of episodes of acute otitis media, and presence of retraction pockets. METHODS: The study included 25 children (41 ears), who had been scheduled for myringotomy with the insertion of tympanostomy tubes due to otitis media with effusion. The interview (duration of the illness, number of episodes of acute otitis media), clinical and audiological examination was conducted according to the developed examination check list. Middle ear effusions (MEE) were collected aseptically, the samples centrifuged and supernatant frozen at a temperature of -80 degrees C. The IL-1beta, IL-6 and IL-10 were assayed using enzyme-linked immunosorbent assay (ELISA) kits incorporating monoclonal antibodies and the ETI system reader. The nonparametric Mann-Whitney U test was used for statistical analysis and Pearson's linear correlation coefficient was calculated. RESULTS: IL-1beta was detected in 80% of examined effusions, IL-6 in 78%, and IL-10 in 88%. There was a strong statistical correlation between IL-1beta and IL-6 concentrations (r=0.627, P=0.000), as well as IL-6 and IL-10 (r=0.66, P=0.000) No statistically significant correlation was found between levels of cytokines and clinical parameters. CONCLUSIONS: Proinflammatory and immunoregulatory cytokines participate in middle ear inflammatory response. The lack of direct correlation between IL-1beta and IL-10 may be responsible for chronic character of the disease. As no correlation between the levels of cytokines and clinical parameters was found it seems that the time of duration of inflammation rather than its intensity measured by levels of cytokines is the main factor in development of middle ear mucosa pathology. SN - 0165-5876 UR - https://www.unboundmedicine.com/medline/citation/17976828/Proinflammatory_and_immunoregulatory_cytokines_in_the_middle_ear_effusions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-5876(07)00418-1 DB - PRIME DP - Unbound Medicine ER -