Tags

Type your tag names separated by a space and hit enter

Screening and confirmation of 100 pesticides in food samples by liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(23):3869-82.RC

Abstract

An analytical method for screening, quantitation and confirmation of a group of 100 pesticides in vegetable and fruit samples was developed using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The pesticides studied belonged to different chemical families of herbicides, insecticides and fungicides; some degradation products were included as well. A thorough optimization was performed for each analyte to achieve individual optimum fragmentor and collision energy voltages. Two transitions per parent compound were monitored in a single chromatographic run containing two time segments. A small particle size C(18) column (1.8 microm) was used for the chromatographic separation of the mixture, providing very narrow peaks and allowing an excellent separation of all the analytes in a 30-min period for maximum peak capacity. The method was validated with blank matrices of green pepper, tomato and orange spiked from 0.1 to 100 microg/kg with the pesticide mix. Quantitation was carried out using matrix-matched standard calibration and linearity of response over 3 orders of magnitude was demonstrated (r > 0.99). Limits of detection based on two transitions and ion-ratio requirements ranged between 0.3 and 50 microg/kg. In general, the sensitivity obtained meets the maximum residue levels (MRLs) established by the European Union regulation for food monitoring programs. The analytical performance of the method was evaluated for different types of vegetables and fruits, showing little or no matrix effects, and examples of screening and confirmation of pesticides in these samples are shown here.

Authors+Show Affiliations

Pesticide Residue Research Group, University of Almeria, Almeria, Spain. iferrer@ual.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17979221

Citation

Ferrer, Imma, et al. "Screening and Confirmation of 100 Pesticides in Food Samples By Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 23, 2007, pp. 3869-82.
Ferrer I, Thurman EM, Zweigenbaum JA. Screening and confirmation of 100 pesticides in food samples by liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(23):3869-82.
Ferrer, I., Thurman, E. M., & Zweigenbaum, J. A. (2007). Screening and confirmation of 100 pesticides in food samples by liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(23), 3869-82.
Ferrer I, Thurman EM, Zweigenbaum JA. Screening and Confirmation of 100 Pesticides in Food Samples By Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(23):3869-82. PubMed PMID: 17979221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening and confirmation of 100 pesticides in food samples by liquid chromatography/tandem mass spectrometry. AU - Ferrer,Imma, AU - Thurman,E Michael, AU - Zweigenbaum,Jerry A, PY - 2007/11/6/pubmed PY - 2008/1/9/medline PY - 2007/11/6/entrez SP - 3869 EP - 82 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 23 N2 - An analytical method for screening, quantitation and confirmation of a group of 100 pesticides in vegetable and fruit samples was developed using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The pesticides studied belonged to different chemical families of herbicides, insecticides and fungicides; some degradation products were included as well. A thorough optimization was performed for each analyte to achieve individual optimum fragmentor and collision energy voltages. Two transitions per parent compound were monitored in a single chromatographic run containing two time segments. A small particle size C(18) column (1.8 microm) was used for the chromatographic separation of the mixture, providing very narrow peaks and allowing an excellent separation of all the analytes in a 30-min period for maximum peak capacity. The method was validated with blank matrices of green pepper, tomato and orange spiked from 0.1 to 100 microg/kg with the pesticide mix. Quantitation was carried out using matrix-matched standard calibration and linearity of response over 3 orders of magnitude was demonstrated (r > 0.99). Limits of detection based on two transitions and ion-ratio requirements ranged between 0.3 and 50 microg/kg. In general, the sensitivity obtained meets the maximum residue levels (MRLs) established by the European Union regulation for food monitoring programs. The analytical performance of the method was evaluated for different types of vegetables and fruits, showing little or no matrix effects, and examples of screening and confirmation of pesticides in these samples are shown here. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17979221/Screening_and_confirmation_of_100_pesticides_in_food_samples_by_liquid_chromatography/tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -