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Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein: implications for Malpighian tubule function during interactions with wheat.
J Insect Physiol. 2008 Jan; 54(1):146-54.JI

Abstract

We report on the transcriptional patterns of a putative white (w) gene encoding an ABC-active-transporter protein during development in Hessian fly, Mayetiola destructor. The deduced amino acid sequence for the Hessian fly white showed 74-77% similarities to white/ATP-binding-cassette proteins and 52-57% similarities to scarlet/ATP-binding-cassette proteins from other dipterans. Conserved ATP-binding motifs and transmembrane alpha-helix segments were identified in the Hessian fly white protein further supporting its function as an ABC-active-transporter similar to the Drosophila white protein. Spatial analysis of transcript levels for white in larval Hessian fly tissues by quantitative real-time PCR revealed the greatest level of transcript in the Malpighian tubules, while analysis of temporal expression during development revealed the highest transcript levels in late 2nd- and early 3rd-instar larvae. Analysis of transcript levels for white in Hessian fly larvae feeding on susceptible and resistant wheat showed greater levels of the transcript in larvae feeding on resistant plants. We speculate the increased transcript level for white in larvae feeding on resistant wheat could be correlated with stress and increased Malpighian tubule activity associated with the metabolism and detoxification of toxic substrates generated either endogenously or encountered exogenously from the host plant.

Authors+Show Affiliations

USDA-ARS, Department of Entomology, Purdue University, West Lafayette, IN 47907, USA. shukle@purdue.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17980386

Citation

Shukle, Richard H., et al. "Tissue and Developmental Expression of a Gene From Hessian Fly Encoding an ABC-active-transporter Protein: Implications for Malpighian Tubule Function During Interactions With Wheat." Journal of Insect Physiology, vol. 54, no. 1, 2008, pp. 146-54.
Shukle RH, Yoshiyama M, Morton PK, et al. Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein: implications for Malpighian tubule function during interactions with wheat. J Insect Physiol. 2008;54(1):146-54.
Shukle, R. H., Yoshiyama, M., Morton, P. K., Johnson, A. J., & Schemerhorn, B. J. (2008). Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein: implications for Malpighian tubule function during interactions with wheat. Journal of Insect Physiology, 54(1), 146-54.
Shukle RH, et al. Tissue and Developmental Expression of a Gene From Hessian Fly Encoding an ABC-active-transporter Protein: Implications for Malpighian Tubule Function During Interactions With Wheat. J Insect Physiol. 2008;54(1):146-54. PubMed PMID: 17980386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein: implications for Malpighian tubule function during interactions with wheat. AU - Shukle,Richard H, AU - Yoshiyama,Mikio, AU - Morton,Philip K, AU - Johnson,Alisha J, AU - Schemerhorn,Brandon J, Y1 - 2007/09/06/ PY - 2007/06/13/received PY - 2007/08/30/revised PY - 2007/08/30/accepted PY - 2007/11/6/pubmed PY - 2008/4/24/medline PY - 2007/11/6/entrez SP - 146 EP - 54 JF - Journal of insect physiology JO - J Insect Physiol VL - 54 IS - 1 N2 - We report on the transcriptional patterns of a putative white (w) gene encoding an ABC-active-transporter protein during development in Hessian fly, Mayetiola destructor. The deduced amino acid sequence for the Hessian fly white showed 74-77% similarities to white/ATP-binding-cassette proteins and 52-57% similarities to scarlet/ATP-binding-cassette proteins from other dipterans. Conserved ATP-binding motifs and transmembrane alpha-helix segments were identified in the Hessian fly white protein further supporting its function as an ABC-active-transporter similar to the Drosophila white protein. Spatial analysis of transcript levels for white in larval Hessian fly tissues by quantitative real-time PCR revealed the greatest level of transcript in the Malpighian tubules, while analysis of temporal expression during development revealed the highest transcript levels in late 2nd- and early 3rd-instar larvae. Analysis of transcript levels for white in Hessian fly larvae feeding on susceptible and resistant wheat showed greater levels of the transcript in larvae feeding on resistant plants. We speculate the increased transcript level for white in larvae feeding on resistant wheat could be correlated with stress and increased Malpighian tubule activity associated with the metabolism and detoxification of toxic substrates generated either endogenously or encountered exogenously from the host plant. SN - 0022-1910 UR - https://www.unboundmedicine.com/medline/citation/17980386/Tissue_and_developmental_expression_of_a_gene_from_Hessian_fly_encoding_an_ABC_active_transporter_protein:_implications_for_Malpighian_tubule_function_during_interactions_with_wheat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(07)00196-5 DB - PRIME DP - Unbound Medicine ER -