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Protective effect of basil (Ocimum basilicum L.) against oxidative DNA damage and mutagenesis.
Food Chem Toxicol. 2008 Feb; 46(2):724-32.FC

Abstract

Mutagenic and antimutagenic properties of essential oil (EO) of basil and its major constituent Linalool, reported to possess antioxidative properties, were examined in microbial tests. In Salmonella/microsome and Escherichia. coli WP2 reversion assays both derivatives (0.25-2.0 microl/plate) showed no mutagenic effect. Salmonella. typhimurium TA98, TA100 and TA102 strains displayed similar sensitivity to both basil derivatives as non-permeable E. coli WP2 strains IC185 and IC202 oxyR. Moreover, the toxicity of basil derivatives to WP2 strains did not depend on OxyR function. The reduction of t-BOOH-induced mutagenesis by EO and Linalool (30-60%) was obtained in repair proficient strains of the E. coli K12 assay (Nikolić, B., Stanojević, J., Mitić, D., Vuković-Gacić, B., Knezević-Vukcević, J., Simić, D., 2004. Comparative study of the antimutagenic potential of vitamin E in different E. coli strains. Mutat. Res. 564, 31-38), as well as in E. coli WP2 IC202 strain. EO and Linalool reduced spontaneous mutagenesis in mismatch repair deficient E. coli K12 strains (27-44%). In all tests, antimutagenic effect of basil derivatives was comparable with that obtained with model antioxidant vitamin E. Linalool and vitamin E induced DNA strand breaks in Comet assay on S. cerevisiae 3A cells, but at non-genotoxic concentrations (0.075 and 0.025 microg/ml, respectively) they reduced the number of H(2)O(2)-induced comets (45-70% Linalool and 80-93% vitamin E). Obtained results indicate that antigenotoxic potential of basil derivatives could be attributed to their antioxidative properties.

Authors+Show Affiliations

Faculty of Biology, Laboratory for Microbiology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. tanjab@bfbot.bg.ac.yuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17980946

Citation

Berić, Tanja, et al. "Protective Effect of Basil (Ocimum Basilicum L.) Against Oxidative DNA Damage and Mutagenesis." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 46, no. 2, 2008, pp. 724-32.
Berić T, Nikolić B, Stanojević J, et al. Protective effect of basil (Ocimum basilicum L.) against oxidative DNA damage and mutagenesis. Food Chem Toxicol. 2008;46(2):724-32.
Berić, T., Nikolić, B., Stanojević, J., Vuković-Gacić, B., & Knezević-Vukcević, J. (2008). Protective effect of basil (Ocimum basilicum L.) against oxidative DNA damage and mutagenesis. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 46(2), 724-32.
Berić T, et al. Protective Effect of Basil (Ocimum Basilicum L.) Against Oxidative DNA Damage and Mutagenesis. Food Chem Toxicol. 2008;46(2):724-32. PubMed PMID: 17980946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of basil (Ocimum basilicum L.) against oxidative DNA damage and mutagenesis. AU - Berić,Tanja, AU - Nikolić,Biljana, AU - Stanojević,Jasna, AU - Vuković-Gacić,Branka, AU - Knezević-Vukcević,Jelena, Y1 - 2007/11/05/ PY - 2007/01/17/received PY - 2007/08/13/revised PY - 2007/09/19/accepted PY - 2007/11/6/pubmed PY - 2008/3/21/medline PY - 2007/11/6/entrez SP - 724 EP - 32 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 46 IS - 2 N2 - Mutagenic and antimutagenic properties of essential oil (EO) of basil and its major constituent Linalool, reported to possess antioxidative properties, were examined in microbial tests. In Salmonella/microsome and Escherichia. coli WP2 reversion assays both derivatives (0.25-2.0 microl/plate) showed no mutagenic effect. Salmonella. typhimurium TA98, TA100 and TA102 strains displayed similar sensitivity to both basil derivatives as non-permeable E. coli WP2 strains IC185 and IC202 oxyR. Moreover, the toxicity of basil derivatives to WP2 strains did not depend on OxyR function. The reduction of t-BOOH-induced mutagenesis by EO and Linalool (30-60%) was obtained in repair proficient strains of the E. coli K12 assay (Nikolić, B., Stanojević, J., Mitić, D., Vuković-Gacić, B., Knezević-Vukcević, J., Simić, D., 2004. Comparative study of the antimutagenic potential of vitamin E in different E. coli strains. Mutat. Res. 564, 31-38), as well as in E. coli WP2 IC202 strain. EO and Linalool reduced spontaneous mutagenesis in mismatch repair deficient E. coli K12 strains (27-44%). In all tests, antimutagenic effect of basil derivatives was comparable with that obtained with model antioxidant vitamin E. Linalool and vitamin E induced DNA strand breaks in Comet assay on S. cerevisiae 3A cells, but at non-genotoxic concentrations (0.075 and 0.025 microg/ml, respectively) they reduced the number of H(2)O(2)-induced comets (45-70% Linalool and 80-93% vitamin E). Obtained results indicate that antigenotoxic potential of basil derivatives could be attributed to their antioxidative properties. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17980946/Protective_effect_of_basil__Ocimum_basilicum_L___against_oxidative_DNA_damage_and_mutagenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(07)00452-8 DB - PRIME DP - Unbound Medicine ER -