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C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion.
Surgery. 2007 Nov; 142(5):722-33.S

Abstract

BACKGROUND

The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh) on this complement activation along with other inflammatory mediators was also studied.

METHODS

Rats were subjected to 1 h of superior mesenteric artery occlusion and 3 h of reperfusion. Intravenous administration of vehicle (human albumin) or C1-Inh (200 U/kg) was performed before (n = 8) or after ischemia (n = 8). II/R increased levels of C4b/c, CRP, IgM, anti-Pc IgM, and myeloperoxidase activity in the intestinal homogenates and induced vascular leakage.

RESULTS

A good correlation was observed in the intestinal homogenates between C4b/c and CRP levels. Clear depositions of C3, CRP, and IgM in intestinal tissue were demonstrated after II/R, and a strong correlation of both CRP and IgM with complement was observed. C1-Inh administered before ischemia reduced the complement activation response after II/R, as reflected by decreased levels of C4b/c in conjunction with reduced anti-Pc IgM in the intestinal homogenates. C1-Inh also decreased leakage of albumin when administered before ischemia. C1-Inh after ischemia reduced C4b/c levels and myeloperoxidase activity in the homogenates.

CONCLUSIONS

CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation. Furthermore, C1-Inh inhibited potentially II/R injury either administered before or after ischemia, by attenuating complement activation induced by CRP and/or natural IgM antibodies.

Authors+Show Affiliations

Department of Immunopathology, Sanquin Research, Plesmalaan 125, Amsterdam, The Netherlands. niubel@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17981193

Citation

Padilla, Niubel Diaz, et al. "C-reactive Protein and Natural IgM Antibodies Are Activators of Complement in a Rat Model of Intestinal Ischemia and Reperfusion." Surgery, vol. 142, no. 5, 2007, pp. 722-33.
Padilla ND, van Vliet AK, Schoots IG, et al. C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion. Surgery. 2007;142(5):722-33.
Padilla, N. D., van Vliet, A. K., Schoots, I. G., Valls Seron, M., Maas, M. A., Peltenburg, E. E., de Vries, A., Niessen, H. W., Hack, C. E., & van Gulik, T. M. (2007). C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion. Surgery, 142(5), 722-33.
Padilla ND, et al. C-reactive Protein and Natural IgM Antibodies Are Activators of Complement in a Rat Model of Intestinal Ischemia and Reperfusion. Surgery. 2007;142(5):722-33. PubMed PMID: 17981193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion. AU - Padilla,Niubel Diaz, AU - van Vliet,Arlène K, AU - Schoots,Ivo G, AU - Valls Seron,Mercedes, AU - Maas,M Adrie, AU - Peltenburg,Esther E Posno, AU - de Vries,Annebeth, AU - Niessen,Hans W M, AU - Hack,C Erik, AU - van Gulik,Thomas M, PY - 2006/11/13/received PY - 2007/05/09/revised PY - 2007/05/11/accepted PY - 2007/11/6/pubmed PY - 2007/12/20/medline PY - 2007/11/6/entrez SP - 722 EP - 33 JF - Surgery JO - Surgery VL - 142 IS - 5 N2 - BACKGROUND: The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh) on this complement activation along with other inflammatory mediators was also studied. METHODS: Rats were subjected to 1 h of superior mesenteric artery occlusion and 3 h of reperfusion. Intravenous administration of vehicle (human albumin) or C1-Inh (200 U/kg) was performed before (n = 8) or after ischemia (n = 8). II/R increased levels of C4b/c, CRP, IgM, anti-Pc IgM, and myeloperoxidase activity in the intestinal homogenates and induced vascular leakage. RESULTS: A good correlation was observed in the intestinal homogenates between C4b/c and CRP levels. Clear depositions of C3, CRP, and IgM in intestinal tissue were demonstrated after II/R, and a strong correlation of both CRP and IgM with complement was observed. C1-Inh administered before ischemia reduced the complement activation response after II/R, as reflected by decreased levels of C4b/c in conjunction with reduced anti-Pc IgM in the intestinal homogenates. C1-Inh also decreased leakage of albumin when administered before ischemia. C1-Inh after ischemia reduced C4b/c levels and myeloperoxidase activity in the homogenates. CONCLUSIONS: CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation. Furthermore, C1-Inh inhibited potentially II/R injury either administered before or after ischemia, by attenuating complement activation induced by CRP and/or natural IgM antibodies. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/17981193/C_reactive_protein_and_natural_IgM_antibodies_are_activators_of_complement_in_a_rat_model_of_intestinal_ischemia_and_reperfusion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(07)00356-X DB - PRIME DP - Unbound Medicine ER -