Tags

Type your tag names separated by a space and hit enter

Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis.
Am J Trop Med Hyg. 2007 Nov; 77(5):814-7.AJ

Abstract

The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with culture-proven melioidosis (sensitivity 61.0%, 95% CI: 49.2-72.0%). PCR was negative for all 306 patients who were culture negative for B. pseudomallei (specificity 100%, 95% CI: 98.8-100%). Diagnostic sensitivity of PCR was 22.7% for patients who were culture positive for blood only, compared with 79.4% for patients who were culture positive for samples other than blood. The median (interquartile range) B. pseudomallei colony count in blood for 44 of 77 patients with positive blood cultures was 2.4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting.

Authors+Show Affiliations

Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. narisara@tropmedres.acNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17984332

Citation

Chantratita, Narisara, et al. "Prospective Clinical Evaluation of the Accuracy of 16S rRNA Real-time PCR Assay for the Diagnosis of Melioidosis." The American Journal of Tropical Medicine and Hygiene, vol. 77, no. 5, 2007, pp. 814-7.
Chantratita N, Wuthiekanun V, Limmathurotsakul D, et al. Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis. Am J Trop Med Hyg. 2007;77(5):814-7.
Chantratita, N., Wuthiekanun, V., Limmathurotsakul, D., Thanwisai, A., Chantratita, W., Day, N. P., & Peacock, S. J. (2007). Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis. The American Journal of Tropical Medicine and Hygiene, 77(5), 814-7.
Chantratita N, et al. Prospective Clinical Evaluation of the Accuracy of 16S rRNA Real-time PCR Assay for the Diagnosis of Melioidosis. Am J Trop Med Hyg. 2007;77(5):814-7. PubMed PMID: 17984332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis. AU - Chantratita,Narisara, AU - Wuthiekanun,Vanaporn, AU - Limmathurotsakul,Direk, AU - Thanwisai,Aunchalee, AU - Chantratita,Wasun, AU - Day,Nicholas P J, AU - Peacock,Sharon J, PY - 2007/11/7/pubmed PY - 2008/5/2/medline PY - 2007/11/7/entrez SP - 814 EP - 7 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 77 IS - 5 N2 - The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with culture-proven melioidosis (sensitivity 61.0%, 95% CI: 49.2-72.0%). PCR was negative for all 306 patients who were culture negative for B. pseudomallei (specificity 100%, 95% CI: 98.8-100%). Diagnostic sensitivity of PCR was 22.7% for patients who were culture positive for blood only, compared with 79.4% for patients who were culture positive for samples other than blood. The median (interquartile range) B. pseudomallei colony count in blood for 44 of 77 patients with positive blood cultures was 2.4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/17984332/Prospective_clinical_evaluation_of_the_accuracy_of_16S_rRNA_real_time_PCR_assay_for_the_diagnosis_of_melioidosis_ DB - PRIME DP - Unbound Medicine ER -