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Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA.
Genet Mol Res. 2007 Aug 07; 6(3):476-81.GM

Abstract

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li's similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43. The local variety, Chenab-70, showed the lowest similarity with the exotic types. We conclude that random amplified polymorphic DNA analysis can be used for the characterization and grouping of wheat genotypes; these results will be helpful in our wheat breeding program.

Authors+Show Affiliations

Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17985299

Citation

Iqbal, A, et al. "Study of Genetic Divergence Among Wheat Genotypes Through Random Amplified Polymorphic DNA." Genetics and Molecular Research : GMR, vol. 6, no. 3, 2007, pp. 476-81.
Iqbal A, Khan AS, Khan IA, et al. Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA. Genet Mol Res. 2007;6(3):476-81.
Iqbal, A., Khan, A. S., Khan, I. A., Awan, F. S., Ahmad, A., & Khan, A. A. (2007). Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA. Genetics and Molecular Research : GMR, 6(3), 476-81.
Iqbal A, et al. Study of Genetic Divergence Among Wheat Genotypes Through Random Amplified Polymorphic DNA. Genet Mol Res. 2007 Aug 7;6(3):476-81. PubMed PMID: 17985299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA. AU - Iqbal,A, AU - Khan,A S, AU - Khan,I A, AU - Awan,F S, AU - Ahmad,A, AU - Khan,A A, Y1 - 2007/08/07/ PY - 2007/11/7/pubmed PY - 2007/12/7/medline PY - 2007/11/7/entrez SP - 476 EP - 81 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 6 IS - 3 N2 - The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li's similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43. The local variety, Chenab-70, showed the lowest similarity with the exotic types. We conclude that random amplified polymorphic DNA analysis can be used for the characterization and grouping of wheat genotypes; these results will be helpful in our wheat breeding program. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/17985299/Study_of_genetic_divergence_among_wheat_genotypes_through_random_amplified_polymorphic_DNA_ DB - PRIME DP - Unbound Medicine ER -