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Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide.
Genet Mol Res. 2007 Sep 30; 6(3):566-74.GM

Abstract

To ascertain whether used and re-refined lubricant oil absorbed through the skin can produce a genotoxic effect or cytotoxicity in mouse bone marrow cells, we examined the induction of micronucleated erythrocytes of peripheral blood after cutaneous application. Both re-refined and used lubricant oils showed a weak but significant induction of micronucleated polychromatic erythrocytes compared with control, while virgin oil did not show micronucleus induction. Cyclophosphamide (CP) was used not only as positive control but also to compare the sensitivity between intraperitoneal and dermal routes of administration of the test compounds in mice. The efficacy of intraperitoneal injection of CP is well known. On the other hand, dermal exposure is not so common and when CP was diluted in glycerin statistically significant values (P = 0.0036) of micronuclei were also found. Topically applied lubricant oils (virgin, re-refined and used) have the capacity to interfere with mouse bone marrow hematopoiesis evidenced by a statistically significant decrease in the proportion of polychromatic erythrocytes in the peripheral blood. Physical and chemical analysis revealed that used oil is more viscous than other lubricants, suggesting the presence of insoluble compounds, oxidized products and water as well as aromatic hydrocarbons. Used oil differs from other lubricant oils in metal and polyaromatic hydrocarbon content. Re-refined oil revealed a neutral value typical of pure mineral oil. This assay is an important tool to evaluate environmental pollutants that cause genotoxicity and/or cytotoxicity through skin exposure.

Authors+Show Affiliations

Superintendência de Fiscalização, Agência Nacional do Petróleo, Brasília, DF, Brasil.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17985309

Citation

Oliveira-Martins, C R., and C K. Grisolia. "Determination of Micronucleus Frequency By Acridine Orange Fluorescent Staining in Peripheral Blood Reticulocytes of Mice Treated Topically With Different Lubricant Oils and Cyclophosphamide." Genetics and Molecular Research : GMR, vol. 6, no. 3, 2007, pp. 566-74.
Oliveira-Martins CR, Grisolia CK. Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide. Genet Mol Res. 2007;6(3):566-74.
Oliveira-Martins, C. R., & Grisolia, C. K. (2007). Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide. Genetics and Molecular Research : GMR, 6(3), 566-74.
Oliveira-Martins CR, Grisolia CK. Determination of Micronucleus Frequency By Acridine Orange Fluorescent Staining in Peripheral Blood Reticulocytes of Mice Treated Topically With Different Lubricant Oils and Cyclophosphamide. Genet Mol Res. 2007 Sep 30;6(3):566-74. PubMed PMID: 17985309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide. AU - Oliveira-Martins,C R, AU - Grisolia,C K, Y1 - 2007/09/30/ PY - 2007/11/7/pubmed PY - 2007/12/7/medline PY - 2007/11/7/entrez SP - 566 EP - 74 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 6 IS - 3 N2 - To ascertain whether used and re-refined lubricant oil absorbed through the skin can produce a genotoxic effect or cytotoxicity in mouse bone marrow cells, we examined the induction of micronucleated erythrocytes of peripheral blood after cutaneous application. Both re-refined and used lubricant oils showed a weak but significant induction of micronucleated polychromatic erythrocytes compared with control, while virgin oil did not show micronucleus induction. Cyclophosphamide (CP) was used not only as positive control but also to compare the sensitivity between intraperitoneal and dermal routes of administration of the test compounds in mice. The efficacy of intraperitoneal injection of CP is well known. On the other hand, dermal exposure is not so common and when CP was diluted in glycerin statistically significant values (P = 0.0036) of micronuclei were also found. Topically applied lubricant oils (virgin, re-refined and used) have the capacity to interfere with mouse bone marrow hematopoiesis evidenced by a statistically significant decrease in the proportion of polychromatic erythrocytes in the peripheral blood. Physical and chemical analysis revealed that used oil is more viscous than other lubricants, suggesting the presence of insoluble compounds, oxidized products and water as well as aromatic hydrocarbons. Used oil differs from other lubricant oils in metal and polyaromatic hydrocarbon content. Re-refined oil revealed a neutral value typical of pure mineral oil. This assay is an important tool to evaluate environmental pollutants that cause genotoxicity and/or cytotoxicity through skin exposure. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/17985309/Determination_of_micronucleus_frequency_by_acridine_orange_fluorescent_staining_in_peripheral_blood_reticulocytes_of_mice_treated_topically_with_different_lubricant_oils_and_cyclophosphamide_ DB - PRIME DP - Unbound Medicine ER -