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Fast determination of herbicides in waters by ultra-performance liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(22):3585-92.RC

Abstract

A rapid multiresidue method for the analysis of more than 40 herbicides (such as simazine, terbuthylazine and diuron) in waters has been developed and validated by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC/MS/MS). Prior to chromatographic determination, the samples were extracted using a solid-phase extraction procedure. The analysis was performed on an Acquity UPLC BEH C(18) column using a gradient elution profile and a mobile phase consisting of methanol and an aqueous solution of formic acid (0.01%). Other chromatographic and MS/MS parameters were optimised in order to improve selectivity and sensitivity of the analytes. The analytes were detected using electrospray ionisation (ESI)-MS/MS in positive ion mode with multiple reaction monitoring (MRM), optimising parameters such as voltage cone, capillary voltage, source and desolvation temperature, and desolvation and cone gas flow. The optimised method provides a rapid separation (less than 10 min) of the selected herbicides in the assayed matrices, and it was validated by the analysis of spiked blank matrix samples. Good linearity was obtained and the repeatability of the method was less than 20% for the lowest calibration point. The limits of detection ranged from 0.002 to 0.02 microg/L, and the limits of quantification from 0.005 to 0.05 microg/L, which were below the values specified by the European Union. Finally, the method was successfully applied to real environmental samples from Andalusia (southern Spain). Terbuthylazine, simazine, atrazine desisopropyl and desethyl terbuthylazine were the herbicides most frequently found in water samples.

Authors+Show Affiliations

Laboratory of Pesticide Residues LAB, Avda. del Mediterráneo, S/N, Edif. Calablanca Bajo 04009 Almería, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17985354

Citation

Montoro, Elena Pastor, et al. "Fast Determination of Herbicides in Waters By Ultra-performance Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 22, 2007, pp. 3585-92.
Montoro EP, González RR, Frenich AG, et al. Fast determination of herbicides in waters by ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(22):3585-92.
Montoro, E. P., González, R. R., Frenich, A. G., Torres, M. E., & Vidal, J. L. (2007). Fast determination of herbicides in waters by ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(22), 3585-92.
Montoro EP, et al. Fast Determination of Herbicides in Waters By Ultra-performance Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(22):3585-92. PubMed PMID: 17985354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast determination of herbicides in waters by ultra-performance liquid chromatography/tandem mass spectrometry. AU - Montoro,Elena Pastor, AU - González,Roberto Romero, AU - Frenich,Antonia Garrido, AU - Torres,M Elena Hernández, AU - Vidal,Jose Luis Martínez, PY - 2007/11/7/pubmed PY - 2007/12/19/medline PY - 2007/11/7/entrez SP - 3585 EP - 92 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 22 N2 - A rapid multiresidue method for the analysis of more than 40 herbicides (such as simazine, terbuthylazine and diuron) in waters has been developed and validated by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC/MS/MS). Prior to chromatographic determination, the samples were extracted using a solid-phase extraction procedure. The analysis was performed on an Acquity UPLC BEH C(18) column using a gradient elution profile and a mobile phase consisting of methanol and an aqueous solution of formic acid (0.01%). Other chromatographic and MS/MS parameters were optimised in order to improve selectivity and sensitivity of the analytes. The analytes were detected using electrospray ionisation (ESI)-MS/MS in positive ion mode with multiple reaction monitoring (MRM), optimising parameters such as voltage cone, capillary voltage, source and desolvation temperature, and desolvation and cone gas flow. The optimised method provides a rapid separation (less than 10 min) of the selected herbicides in the assayed matrices, and it was validated by the analysis of spiked blank matrix samples. Good linearity was obtained and the repeatability of the method was less than 20% for the lowest calibration point. The limits of detection ranged from 0.002 to 0.02 microg/L, and the limits of quantification from 0.005 to 0.05 microg/L, which were below the values specified by the European Union. Finally, the method was successfully applied to real environmental samples from Andalusia (southern Spain). Terbuthylazine, simazine, atrazine desisopropyl and desethyl terbuthylazine were the herbicides most frequently found in water samples. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17985354/Fast_determination_of_herbicides_in_waters_by_ultra_performance_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3258 DB - PRIME DP - Unbound Medicine ER -