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X-tox: an atypical defensin derived family of immune-related proteins specific to Lepidoptera.
Dev Comp Immunol 2008; 32(5):575-84DC

Abstract

We report here the isolation in Spodoptera frugiperda (Lepidoptera) of an immune-related protein (hereafter named Spod-11-tox), characterized by imperfectly conserved tandem repeats of 11 cysteine-stabilized alpha beta motifs (CS-alphabeta), the structural scaffold characteristic of invertebrate defensins and scorpion toxins. Spod-11-tox orthologs were only found in Lepidopteran species, suggesting that this new protein family (named X-tox) is specific to this insect order. Moreover, phylogenetic analysis suggests that X-tox proteins represent a new class of proteins restricted to Lepidoptera and likely derived from Lepidopteran defensins. In S. frugiperda, analysis of gene expression revealed that spod-11-tox is rapidly induced by infection. However, and conversely to what is known for most insect antimicrobial peptides (AMP), spod-11-tox is mainly expressed in blood cells. Moreover, recombinant Spod-11-tox produced in the Sf9 cell line does not show any antimicrobial activity. Altogether, these results suggest that although X-tox proteins are derived from defensins, they may play a different and still unknown role in Lepidoptera immune response.

Authors+Show Affiliations

Ecologie Microbienne des Insectes et Interactions Hôte-Pathogène, Université de Montpellier II, 34095 Montpellier, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17988734

Citation

Girard, Pierre-Alain, et al. "X-tox: an Atypical Defensin Derived Family of Immune-related Proteins Specific to Lepidoptera." Developmental and Comparative Immunology, vol. 32, no. 5, 2008, pp. 575-84.
Girard PA, Boublik Y, Wheat CW, et al. X-tox: an atypical defensin derived family of immune-related proteins specific to Lepidoptera. Dev Comp Immunol. 2008;32(5):575-84.
Girard, P. A., Boublik, Y., Wheat, C. W., Volkoff, A. N., Cousserans, F., Brehélin, M., & Escoubas, J. M. (2008). X-tox: an atypical defensin derived family of immune-related proteins specific to Lepidoptera. Developmental and Comparative Immunology, 32(5), pp. 575-84.
Girard PA, et al. X-tox: an Atypical Defensin Derived Family of Immune-related Proteins Specific to Lepidoptera. Dev Comp Immunol. 2008;32(5):575-84. PubMed PMID: 17988734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - X-tox: an atypical defensin derived family of immune-related proteins specific to Lepidoptera. AU - Girard,Pierre-Alain, AU - Boublik,Yvan, AU - Wheat,Christopher W, AU - Volkoff,Anne-Nathalie, AU - Cousserans,F, AU - Brehélin,Michel, AU - Escoubas,Jean-Michel, Y1 - 2007/10/12/ PY - 2007/06/29/received PY - 2007/08/28/revised PY - 2007/09/27/accepted PY - 2007/11/9/pubmed PY - 2008/4/30/medline PY - 2007/11/9/entrez SP - 575 EP - 84 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 32 IS - 5 N2 - We report here the isolation in Spodoptera frugiperda (Lepidoptera) of an immune-related protein (hereafter named Spod-11-tox), characterized by imperfectly conserved tandem repeats of 11 cysteine-stabilized alpha beta motifs (CS-alphabeta), the structural scaffold characteristic of invertebrate defensins and scorpion toxins. Spod-11-tox orthologs were only found in Lepidopteran species, suggesting that this new protein family (named X-tox) is specific to this insect order. Moreover, phylogenetic analysis suggests that X-tox proteins represent a new class of proteins restricted to Lepidoptera and likely derived from Lepidopteran defensins. In S. frugiperda, analysis of gene expression revealed that spod-11-tox is rapidly induced by infection. However, and conversely to what is known for most insect antimicrobial peptides (AMP), spod-11-tox is mainly expressed in blood cells. Moreover, recombinant Spod-11-tox produced in the Sf9 cell line does not show any antimicrobial activity. Altogether, these results suggest that although X-tox proteins are derived from defensins, they may play a different and still unknown role in Lepidoptera immune response. SN - 0145-305X UR - https://www.unboundmedicine.com/medline/citation/17988734/X_tox:_an_atypical_defensin_derived_family_of_immune_related_proteins_specific_to_Lepidoptera_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(07)00131-0 DB - PRIME DP - Unbound Medicine ER -