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Evaluation of Escherichia coli O157:H7 in apple juice with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract by conventional media and thin agar layer method.
Food Microbiol. 2008 Feb; 25(1):190-5.FM

Abstract

Escherichia coli O157:H7 survival in apple juice supplemented with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract was studied. Inoculated samples with or without Cornus fruit extract were kept at 21 and 7 degrees C. Microbial analysis was conducted on days 0, 1, 3, 5, and 7. MacConkey sorbitol agar (MSA), tryptic soy agar (TSA), and thin agar layer (TAL) medium were used to compare the recovery of bacteria stressed under combination treatment. Influence of temperature, storage time, and Cornus fruit on survival of cells was evaluated. The most dramatic reduction of E. coli O157:H7 was observed in apple juice with Cornus fruit extract at 21 degrees C. At 7 degrees C, E. coli O157:H7 was reduced by 2.3logcfu/ml in the apple juice with Cornus fruit extract compared to the control sample on day 7. TAL and TSA were more efficient than MSA. Cornus fruit extract can be used in combination with temperature and storage time controls to inactivate E. coli O157:H7 in apple juice. This study has shown that TAL is a viable method of recovering and differentiating injured microorganisms and apple juice supplemented with Cornus fruit has potential as a value-added beverage with antimicrobial effects and potential health benefits.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, The University of Maine, Orono, ME 04469-5735, USA. vivian.wu@umit.maine.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17993394

Citation

Wu, Vivian C H., et al. "Evaluation of Escherichia Coli O157:H7 in Apple Juice With Cornus Fruit (Cornus Officinalis Sieb. Et Zucc.) Extract By Conventional Media and Thin Agar Layer Method." Food Microbiology, vol. 25, no. 1, 2008, pp. 190-5.
Wu VC, Qiu X, Peggy Hsieh YH. Evaluation of Escherichia coli O157:H7 in apple juice with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract by conventional media and thin agar layer method. Food Microbiol. 2008;25(1):190-5.
Wu, V. C., Qiu, X., & Peggy Hsieh, Y. H. (2008). Evaluation of Escherichia coli O157:H7 in apple juice with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract by conventional media and thin agar layer method. Food Microbiology, 25(1), 190-5.
Wu VC, Qiu X, Peggy Hsieh YH. Evaluation of Escherichia Coli O157:H7 in Apple Juice With Cornus Fruit (Cornus Officinalis Sieb. Et Zucc.) Extract By Conventional Media and Thin Agar Layer Method. Food Microbiol. 2008;25(1):190-5. PubMed PMID: 17993394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Escherichia coli O157:H7 in apple juice with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract by conventional media and thin agar layer method. AU - Wu,Vivian C H, AU - Qiu,Xujian, AU - Peggy Hsieh,Y-H, Y1 - 2007/09/21/ PY - 2007/05/07/received PY - 2007/09/07/revised PY - 2007/09/14/accepted PY - 2007/11/13/pubmed PY - 2008/2/12/medline PY - 2007/11/13/entrez SP - 190 EP - 5 JF - Food microbiology JO - Food Microbiol VL - 25 IS - 1 N2 - Escherichia coli O157:H7 survival in apple juice supplemented with Cornus fruit (Cornus officinalis Sieb. et Zucc.) extract was studied. Inoculated samples with or without Cornus fruit extract were kept at 21 and 7 degrees C. Microbial analysis was conducted on days 0, 1, 3, 5, and 7. MacConkey sorbitol agar (MSA), tryptic soy agar (TSA), and thin agar layer (TAL) medium were used to compare the recovery of bacteria stressed under combination treatment. Influence of temperature, storage time, and Cornus fruit on survival of cells was evaluated. The most dramatic reduction of E. coli O157:H7 was observed in apple juice with Cornus fruit extract at 21 degrees C. At 7 degrees C, E. coli O157:H7 was reduced by 2.3logcfu/ml in the apple juice with Cornus fruit extract compared to the control sample on day 7. TAL and TSA were more efficient than MSA. Cornus fruit extract can be used in combination with temperature and storage time controls to inactivate E. coli O157:H7 in apple juice. This study has shown that TAL is a viable method of recovering and differentiating injured microorganisms and apple juice supplemented with Cornus fruit has potential as a value-added beverage with antimicrobial effects and potential health benefits. SN - 0740-0020 UR - https://www.unboundmedicine.com/medline/citation/17993394/Evaluation_of_Escherichia_coli_O157:H7_in_apple_juice_with_Cornus_fruit__Cornus_officinalis_Sieb__et_Zucc___extract_by_conventional_media_and_thin_agar_layer_method_ DB - PRIME DP - Unbound Medicine ER -