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Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice.
Acta Ophthalmol 2008; 86(4):395-400AO

Abstract

PURPOSE

Although the presence of gamma-aminobutyrate acid (GABA) in amacrine cells and its co-localization with other neuronal substances is well known, there exists only little information about their quantitative distribution in the mouse eye. The aim of the present study was to characterize GABA-ergic amacrine cells in the retina of the recently introduced glutamate decarboxylase 67-green fluorescent protein (GAD67-GFP) knock-in mouse.

METHODS

Whole mounts of the retina were prepared and the GFP-positive neurons quantified. Immunofluorescence staining was performed with antibodies against GABA, calbindin (CB), calretinin (CR), parvalbumin (PV), choline acetyl transferase (ChAT), tyrosine hydroxylase (TH), vesicular glutamate transporter (VGluT) 1, VGluT2 and VGluT3.

RESULTS

Displaced GABA-ergic amacrine cells in the ganglion cell layer (GCL) showed a density of 1006 +/- 170 cells/mm(2). In the inner nuclear layer (INL), the density of amacrine cells was 8821 +/- 448 cells/mm(2) in the central region and 6825 +/- 408 cells/mm(2) in the peripheral region. GFP-positive amacrine cells co-localized with GABA (99%), CR (INL 18%, GCL 71.3%), CB (INL 6.3%), bNOS (INL 1%, GCL 4%), and ChAT (INL 17%, GCL 92.6%). No co-localization was seen with antibodies against PV, TH, and VGluT 1-3.

CONCLUSIONS

This study presents the first quantitative data concerning the co-localization of GABA-ergic neurons in the mouse retina with various neuronal markers.

Authors+Show Affiliations

Department of Anatomy, Carl Gustav Carus Medical Faculty, Technical University Dresden, Dresden, Germany. Albrecht.May@mailbox.tu-dresden.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17995983

Citation

May, Christian Albrecht, et al. "Quantification and Characterization of GABA-ergic Amacrine Cells in the Retina of GAD67-GFP Knock-in Mice." Acta Ophthalmologica, vol. 86, no. 4, 2008, pp. 395-400.
May CA, Nakamura K, Fujiyama F, et al. Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice. Acta Ophthalmol. 2008;86(4):395-400.
May, C. A., Nakamura, K., Fujiyama, F., & Yanagawa, Y. (2008). Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice. Acta Ophthalmologica, 86(4), pp. 395-400.
May CA, et al. Quantification and Characterization of GABA-ergic Amacrine Cells in the Retina of GAD67-GFP Knock-in Mice. Acta Ophthalmol. 2008;86(4):395-400. PubMed PMID: 17995983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice. AU - May,Christian Albrecht, AU - Nakamura,Kouichi, AU - Fujiyama,Fumino, AU - Yanagawa,Yuchio, Y1 - 2007/11/07/ PY - 2007/11/13/pubmed PY - 2008/7/24/medline PY - 2007/11/13/entrez SP - 395 EP - 400 JF - Acta ophthalmologica JO - Acta Ophthalmol VL - 86 IS - 4 N2 - PURPOSE: Although the presence of gamma-aminobutyrate acid (GABA) in amacrine cells and its co-localization with other neuronal substances is well known, there exists only little information about their quantitative distribution in the mouse eye. The aim of the present study was to characterize GABA-ergic amacrine cells in the retina of the recently introduced glutamate decarboxylase 67-green fluorescent protein (GAD67-GFP) knock-in mouse. METHODS: Whole mounts of the retina were prepared and the GFP-positive neurons quantified. Immunofluorescence staining was performed with antibodies against GABA, calbindin (CB), calretinin (CR), parvalbumin (PV), choline acetyl transferase (ChAT), tyrosine hydroxylase (TH), vesicular glutamate transporter (VGluT) 1, VGluT2 and VGluT3. RESULTS: Displaced GABA-ergic amacrine cells in the ganglion cell layer (GCL) showed a density of 1006 +/- 170 cells/mm(2). In the inner nuclear layer (INL), the density of amacrine cells was 8821 +/- 448 cells/mm(2) in the central region and 6825 +/- 408 cells/mm(2) in the peripheral region. GFP-positive amacrine cells co-localized with GABA (99%), CR (INL 18%, GCL 71.3%), CB (INL 6.3%), bNOS (INL 1%, GCL 4%), and ChAT (INL 17%, GCL 92.6%). No co-localization was seen with antibodies against PV, TH, and VGluT 1-3. CONCLUSIONS: This study presents the first quantitative data concerning the co-localization of GABA-ergic neurons in the mouse retina with various neuronal markers. SN - 1755-3768 UR - https://www.unboundmedicine.com/medline/citation/17995983/Quantification_and_characterization_of_GABA_ergic_amacrine_cells_in_the_retina_of_GAD67_GFP_knock_in_mice_ L2 - https://doi.org/10.1111/j.1600-0420.2007.01054.x DB - PRIME DP - Unbound Medicine ER -