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[Precondition with doxycycline administration abates ischemia/reperfusion injury and reduces the expression of matrix metalloproteinase-1/9 in myocardium in rat in vivo].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Nov; 19(11):679-82.ZW

Abstract

OBJECTIVE

To investigate the effects of preconditioning with doxycycline on ischemia/reperfusion (I/R) injury and expressions of matrix metalloproteinase-1 (MMP-1) and MMP-9 in myocardium in rat in vivo.

METHODS

Sixty-four healthy male SD rats, weighing 300-350 g, were randomly divided into three groups: sham operation group (n = 16), I/R group (n = 24), and doxycycline preconditioning group (n = 24). The heart function, plasma level of creatine kinase (CK) and lactate dehydrogenase (LDH), and the activity of myeloperoxidase (MPO) were respectively determined in rat myocardium after ischemia for 60 minutes and reperfusion for 120 minutes or with the same procedure but with precondition of Doxycycline. Also the expressions of MMP-1 and MMP-9 was determined by immunohistochemistry.

RESULTS

In I/R group, left ventricular systolic pressure (LVSP) and the maximum and minimum first derivative of LVSP (+/-dp/dtmax) were significantly less, and left ventricular end diastolic pressure (LVEDP), CK, LDH, the activity of MPO, and the protein expressions of MMP-1 and MMP-9 were remarkably higher compared with the sham operation group (P<0.05 or P<0.01). Heart function was improved, CK, LDH, MPO and the expressions of MMP-1 and MMP-9 were reduced obviously by doxycycline preconditioning compared with the I/R group (P<0.05 or P<0.01).

CONCLUSION

Precondition with doxycycline could remarkably abate I/R injury of rat myocardium in vivo and inhibit the expressions of MMP-1 and MMP-9.

Authors+Show Affiliations

Wu LM
Department of Cardiology, The Union Hospital, Fujian Medcial University, Fujian Institute of Senile Disease, Fuzhou 350001, Fujian, China.
Fan L
No affiliation info available
Chen LL
No affiliation info available

MeSH

AnimalsDisease Models, AnimalDoxycyclineIschemic Preconditioning, MyocardialMaleMatrix Metalloproteinase 1Matrix Metalloproteinase 9Myocardial Reperfusion InjuryMyocardiumRandom AllocationRatsRats, Sprague-Dawley

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17996137

Citation

Wu, Li-ming, et al. "[Precondition With Doxycycline Administration Abates Ischemia/reperfusion Injury and Reduces the Expression of Matrix Metalloproteinase-1/9 in Myocardium in Rat in Vivo]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 19, no. 11, 2007, pp. 679-82.
Wu LM, Fan L, Chen LL. [Precondition with doxycycline administration abates ischemia/reperfusion injury and reduces the expression of matrix metalloproteinase-1/9 in myocardium in rat in vivo]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007;19(11):679-82.
Wu, L. M., Fan, L., & Chen, L. L. (2007). [Precondition with doxycycline administration abates ischemia/reperfusion injury and reduces the expression of matrix metalloproteinase-1/9 in myocardium in rat in vivo]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 19(11), 679-82.
Wu LM, Fan L, Chen LL. [Precondition With Doxycycline Administration Abates Ischemia/reperfusion Injury and Reduces the Expression of Matrix Metalloproteinase-1/9 in Myocardium in Rat in Vivo]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007;19(11):679-82. PubMed PMID: 17996137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Precondition with doxycycline administration abates ischemia/reperfusion injury and reduces the expression of matrix metalloproteinase-1/9 in myocardium in rat in vivo]. AU - Wu,Li-ming, AU - Fan,Lin, AU - Chen,Liang-long, PY - 2007/11/13/pubmed PY - 2009/10/9/medline PY - 2007/11/13/entrez SP - 679 EP - 82 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 19 IS - 11 N2 - OBJECTIVE: To investigate the effects of preconditioning with doxycycline on ischemia/reperfusion (I/R) injury and expressions of matrix metalloproteinase-1 (MMP-1) and MMP-9 in myocardium in rat in vivo. METHODS: Sixty-four healthy male SD rats, weighing 300-350 g, were randomly divided into three groups: sham operation group (n = 16), I/R group (n = 24), and doxycycline preconditioning group (n = 24). The heart function, plasma level of creatine kinase (CK) and lactate dehydrogenase (LDH), and the activity of myeloperoxidase (MPO) were respectively determined in rat myocardium after ischemia for 60 minutes and reperfusion for 120 minutes or with the same procedure but with precondition of Doxycycline. Also the expressions of MMP-1 and MMP-9 was determined by immunohistochemistry. RESULTS: In I/R group, left ventricular systolic pressure (LVSP) and the maximum and minimum first derivative of LVSP (+/-dp/dtmax) were significantly less, and left ventricular end diastolic pressure (LVEDP), CK, LDH, the activity of MPO, and the protein expressions of MMP-1 and MMP-9 were remarkably higher compared with the sham operation group (P<0.05 or P<0.01). Heart function was improved, CK, LDH, MPO and the expressions of MMP-1 and MMP-9 were reduced obviously by doxycycline preconditioning compared with the I/R group (P<0.05 or P<0.01). CONCLUSION: Precondition with doxycycline could remarkably abate I/R injury of rat myocardium in vivo and inhibit the expressions of MMP-1 and MMP-9. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/17996137/[Precondition_with_doxycycline_administration_abates_ischemia/reperfusion_injury_and_reduces_the_expression_of_matrix_metalloproteinase_1/9_in_myocardium_in_rat_in_vivo]_ DB - PRIME DP - Unbound Medicine ER -