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Topical application of a novel ceramide derivative, K6PC-9, inhibits dust mite extract-induced atopic dermatitis-like skin lesions in NC/Nga mice.
Int Immunopharmacol. 2007 Dec 15; 7(13):1589-97.II

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease. K6PC-9 (N-Ethanol-2-hexyl-3-oxo-decanamide) is a novel synthetic ceramide derivative of PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide), which was known to be effective in atopic and psoriatic patients. To investigate the immunomodulatory activity of K6PC-9, we examined the effect of K6PC-9 on T lymphocyte and macrophage function and the effect of topical application of K6PC-9 on skin inflammation and AD-like skin lesions in mouse models. K6PC-9 had no effect on concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In contrast, lipopolysaccharide-induced nitrite generation was potently suppressed by K6PC-9 in mouse peritoneal macrophages. In mouse model of skin inflammation, K6PC-9 inhibited phorbol ester-induced increase in ear thickness and expression of tumor necrosis factor-alpha in the ear of BALB/c mice. Topical application of K6PC-9 also suppressed mite extract-induced AD-like skin lesions in NC/Nga mice. Increase in ear thickness was significantly inhibited by K6PC-9 in this model. K6PC-9 also blocked the infiltration of mast cells and neutrophils into the ear. Further study demonstrated that the mRNA expression of tumor necrosis factor-alpha and adhesion molecules, such as vascular cell adhesion molecule-1, intercellular adhesion molecule-1, E-selectin, was also suppressed by K6PC-9 in the ear of mite extract-treated NC/Nga mice. Taken together, the results presented in this report show that K6PC-9 has an anti-inflammatory potential and exerts beneficial effects in an animal model of AD, indicating that K6PC-9 might be used as a topical agent for the treatment of AD.

Authors+Show Affiliations

Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon, Chungbuk, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17996668

Citation

Kang, Jong Soon, et al. "Topical Application of a Novel Ceramide Derivative, K6PC-9, Inhibits Dust Mite Extract-induced Atopic Dermatitis-like Skin Lesions in NC/Nga Mice." International Immunopharmacology, vol. 7, no. 13, 2007, pp. 1589-97.
Kang JS, Youm JK, Jeong SK, et al. Topical application of a novel ceramide derivative, K6PC-9, inhibits dust mite extract-induced atopic dermatitis-like skin lesions in NC/Nga mice. Int Immunopharmacol. 2007;7(13):1589-97.
Kang, J. S., Youm, J. K., Jeong, S. K., Park, B. D., Yoon, W. K., Han, M. H., Lee, H., Han, S. B., Lee, K., Park, S. K., Lee, S. H., Yang, K. H., Moon, E. Y., & Kim, H. M. (2007). Topical application of a novel ceramide derivative, K6PC-9, inhibits dust mite extract-induced atopic dermatitis-like skin lesions in NC/Nga mice. International Immunopharmacology, 7(13), 1589-97.
Kang JS, et al. Topical Application of a Novel Ceramide Derivative, K6PC-9, Inhibits Dust Mite Extract-induced Atopic Dermatitis-like Skin Lesions in NC/Nga Mice. Int Immunopharmacol. 2007 Dec 15;7(13):1589-97. PubMed PMID: 17996668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Topical application of a novel ceramide derivative, K6PC-9, inhibits dust mite extract-induced atopic dermatitis-like skin lesions in NC/Nga mice. AU - Kang,Jong Soon, AU - Youm,Jong-Kyung, AU - Jeong,Se Kyoo, AU - Park,Byeong Deog, AU - Yoon,Won Kee, AU - Han,Mi Hwa, AU - Lee,Hyunju, AU - Han,Sang-Bae, AU - Lee,Kiho, AU - Park,Song-Kyu, AU - Lee,Seung Hun, AU - Yang,Kyu-Hwan, AU - Moon,Eun-Yi, AU - Kim,Hwan Mook, Y1 - 2007/09/04/ PY - 2007/04/26/received PY - 2007/07/20/revised PY - 2007/08/03/accepted PY - 2007/11/13/pubmed PY - 2008/1/25/medline PY - 2007/11/13/entrez SP - 1589 EP - 97 JF - International immunopharmacology JO - Int Immunopharmacol VL - 7 IS - 13 N2 - Atopic dermatitis (AD) is a chronic inflammatory skin disease. K6PC-9 (N-Ethanol-2-hexyl-3-oxo-decanamide) is a novel synthetic ceramide derivative of PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide), which was known to be effective in atopic and psoriatic patients. To investigate the immunomodulatory activity of K6PC-9, we examined the effect of K6PC-9 on T lymphocyte and macrophage function and the effect of topical application of K6PC-9 on skin inflammation and AD-like skin lesions in mouse models. K6PC-9 had no effect on concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In contrast, lipopolysaccharide-induced nitrite generation was potently suppressed by K6PC-9 in mouse peritoneal macrophages. In mouse model of skin inflammation, K6PC-9 inhibited phorbol ester-induced increase in ear thickness and expression of tumor necrosis factor-alpha in the ear of BALB/c mice. Topical application of K6PC-9 also suppressed mite extract-induced AD-like skin lesions in NC/Nga mice. Increase in ear thickness was significantly inhibited by K6PC-9 in this model. K6PC-9 also blocked the infiltration of mast cells and neutrophils into the ear. Further study demonstrated that the mRNA expression of tumor necrosis factor-alpha and adhesion molecules, such as vascular cell adhesion molecule-1, intercellular adhesion molecule-1, E-selectin, was also suppressed by K6PC-9 in the ear of mite extract-treated NC/Nga mice. Taken together, the results presented in this report show that K6PC-9 has an anti-inflammatory potential and exerts beneficial effects in an animal model of AD, indicating that K6PC-9 might be used as a topical agent for the treatment of AD. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/17996668/Topical_application_of_a_novel_ceramide_derivative_K6PC_9_inhibits_dust_mite_extract_induced_atopic_dermatitis_like_skin_lesions_in_NC/Nga_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(07)00240-8 DB - PRIME DP - Unbound Medicine ER -