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Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model.
Biosens Bioelectron. 2008 Feb 28; 23(7):965-70.BB

Abstract

Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated.

Authors+Show Affiliations

Li B
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, PR China.
Wang Y
No affiliation info available
Wei H
No affiliation info available
Dong S
No affiliation info available

MeSH

Amplifiers, ElectronicAptamers, NucleotideBiosensing TechniquesCoated Materials, BiocompatibleElectrochemistryEquipment DesignEquipment Failure AnalysisGoldNanoparticlesParticle SizeReproducibility of ResultsSensitivity and SpecificityThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17997091

Citation

Li, Bingling, et al. "Amplified Electrochemical Aptasensor Taking AuNPs Based Sandwich Sensing Platform as a Model." Biosensors & Bioelectronics, vol. 23, no. 7, 2008, pp. 965-70.
Li B, Wang Y, Wei H, et al. Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. Biosens Bioelectron. 2008;23(7):965-70.
Li, B., Wang, Y., Wei, H., & Dong, S. (2008). Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. Biosensors & Bioelectronics, 23(7), 965-70.
Li B, et al. Amplified Electrochemical Aptasensor Taking AuNPs Based Sandwich Sensing Platform as a Model. Biosens Bioelectron. 2008 Feb 28;23(7):965-70. PubMed PMID: 17997091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. AU - Li,Bingling, AU - Wang,Yuling, AU - Wei,Hui, AU - Dong,Shaojun, Y1 - 2007/10/02/ PY - 2007/06/22/received PY - 2007/09/19/revised PY - 2007/09/27/accepted PY - 2007/11/13/pubmed PY - 2008/5/7/medline PY - 2007/11/13/entrez SP - 965 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 7 N2 - Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17997091/Amplified_electrochemical_aptasensor_taking_AuNPs_based_sandwich_sensing_platform_as_a_model_ DB - PRIME DP - Unbound Medicine ER -