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Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model.
Biosens Bioelectron. 2008 Feb 28; 23(7):965-70.BB

Abstract

Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated.

Authors+Show Affiliations

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17997091

Citation

Li, Bingling, et al. "Amplified Electrochemical Aptasensor Taking AuNPs Based Sandwich Sensing Platform as a Model." Biosensors & Bioelectronics, vol. 23, no. 7, 2008, pp. 965-70.
Li B, Wang Y, Wei H, et al. Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. Biosens Bioelectron. 2008;23(7):965-70.
Li, B., Wang, Y., Wei, H., & Dong, S. (2008). Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. Biosensors & Bioelectronics, 23(7), 965-70.
Li B, et al. Amplified Electrochemical Aptasensor Taking AuNPs Based Sandwich Sensing Platform as a Model. Biosens Bioelectron. 2008 Feb 28;23(7):965-70. PubMed PMID: 17997091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model. AU - Li,Bingling, AU - Wang,Yuling, AU - Wei,Hui, AU - Dong,Shaojun, Y1 - 2007/10/02/ PY - 2007/06/22/received PY - 2007/09/19/revised PY - 2007/09/27/accepted PY - 2007/11/13/pubmed PY - 2008/5/7/medline PY - 2007/11/13/entrez SP - 965 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 7 N2 - Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17997091/Amplified_electrochemical_aptasensor_taking_AuNPs_based_sandwich_sensing_platform_as_a_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00413-7 DB - PRIME DP - Unbound Medicine ER -