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Assessment of the genotoxic potential of benzo(a)pyrene and pp'-dichlorodiphenyldichloroethylene in Zebra mussel (Dreissena polymorpha).
Mutat Res. 2008 Jan 08; 649(1-2):135-45.MR

Abstract

The single-cell gel electrophoresis (SCGE) assay and the micronucleus (MN) test were carried out with haemocytes of Zebra mussel (Dreissena polymorpha) specimens to evaluate the potential genotoxicity of benzo(a)pyrene (BaP) and pp'-dichlorodiphenyldichloroethylene (pp'-DDE, a metabolite of pp'-DDT). Mussels were exposed to three different concentrations (0.1 microg/L, 2 microg/L, 10 microg/L) of each chemical in water during 168 h (SCGE assay) and 96 h (MN test) of exposure under laboratory conditions. These levels correspond to nominal molar concentrations of 0.4 nM, 7.9 nM and 40 nM for BaP and 0.3 nM, 6.2 nM and 31 nM for pp'-DDE, respectively. Concurrently, the levels of toxicants were measured in soft tissues of the mussels by gas-chromatographic analyses, to evaluate their temporal trends and the dose/response relationships. Significant increases of the ratio between the comet length and the diameter of the comet head (LDR) and of micronucleus frequencies in comparison with baseline levels were observed not only for all concentrations of BaP, but also for pp'-DDE (except 0.3 nM). The concentration above which DNA damage starts to be significantly increased was 0.8 nmol/g lipids for BaP and 1.6 nmol/g lipids for pp'-DDE, respectively. The results of these experiments show a clear genotoxic effect on this non-target organism not only for the well-known genotoxicant BaP, but also for the final metabolite of pp'-DDT at soft-tissue concentrations that have been found in several aquatic ecosystems worldwide.

Authors+Show Affiliations

Department of Biology, University of Milan, Via Celoria 26, Milan, Italy. andrea.binelli@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17997130

Citation

Binelli, Andrea, et al. "Assessment of the Genotoxic Potential of Benzo(a)pyrene and Pp'-dichlorodiphenyldichloroethylene in Zebra Mussel (Dreissena Polymorpha)." Mutation Research, vol. 649, no. 1-2, 2008, pp. 135-45.
Binelli A, Riva C, Cogni D, et al. Assessment of the genotoxic potential of benzo(a)pyrene and pp'-dichlorodiphenyldichloroethylene in Zebra mussel (Dreissena polymorpha). Mutat Res. 2008;649(1-2):135-45.
Binelli, A., Riva, C., Cogni, D., & Provini, A. (2008). Assessment of the genotoxic potential of benzo(a)pyrene and pp'-dichlorodiphenyldichloroethylene in Zebra mussel (Dreissena polymorpha). Mutation Research, 649(1-2), 135-45.
Binelli A, et al. Assessment of the Genotoxic Potential of Benzo(a)pyrene and Pp'-dichlorodiphenyldichloroethylene in Zebra Mussel (Dreissena Polymorpha). Mutat Res. 2008 Jan 8;649(1-2):135-45. PubMed PMID: 17997130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of the genotoxic potential of benzo(a)pyrene and pp'-dichlorodiphenyldichloroethylene in Zebra mussel (Dreissena polymorpha). AU - Binelli,Andrea, AU - Riva,Consuelo, AU - Cogni,Daniele, AU - Provini,Alfredo, Y1 - 2007/10/06/ PY - 2007/03/27/received PY - 2007/07/07/revised PY - 2007/08/15/accepted PY - 2007/11/13/pubmed PY - 2008/5/31/medline PY - 2007/11/13/entrez SP - 135 EP - 45 JF - Mutation research JO - Mutat Res VL - 649 IS - 1-2 N2 - The single-cell gel electrophoresis (SCGE) assay and the micronucleus (MN) test were carried out with haemocytes of Zebra mussel (Dreissena polymorpha) specimens to evaluate the potential genotoxicity of benzo(a)pyrene (BaP) and pp'-dichlorodiphenyldichloroethylene (pp'-DDE, a metabolite of pp'-DDT). Mussels were exposed to three different concentrations (0.1 microg/L, 2 microg/L, 10 microg/L) of each chemical in water during 168 h (SCGE assay) and 96 h (MN test) of exposure under laboratory conditions. These levels correspond to nominal molar concentrations of 0.4 nM, 7.9 nM and 40 nM for BaP and 0.3 nM, 6.2 nM and 31 nM for pp'-DDE, respectively. Concurrently, the levels of toxicants were measured in soft tissues of the mussels by gas-chromatographic analyses, to evaluate their temporal trends and the dose/response relationships. Significant increases of the ratio between the comet length and the diameter of the comet head (LDR) and of micronucleus frequencies in comparison with baseline levels were observed not only for all concentrations of BaP, but also for pp'-DDE (except 0.3 nM). The concentration above which DNA damage starts to be significantly increased was 0.8 nmol/g lipids for BaP and 1.6 nmol/g lipids for pp'-DDE, respectively. The results of these experiments show a clear genotoxic effect on this non-target organism not only for the well-known genotoxicant BaP, but also for the final metabolite of pp'-DDT at soft-tissue concentrations that have been found in several aquatic ecosystems worldwide. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/17997130/Assessment_of_the_genotoxic_potential_of_benzo_a_pyrene_and_pp'_dichlorodiphenyldichloroethylene_in_Zebra_mussel__Dreissena_polymorpha__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(07)00283-5 DB - PRIME DP - Unbound Medicine ER -