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Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vitro.
Biol Cell. 2008 Apr; 100(4):231-41.BC

Abstract

BACKGROUND INFORMATION

DMD (Duchenne muscular dystrophy) is a devastating X-linked disorder characterized by progressive muscle degeneration and weakness. The use of cell therapy for the repair of defective muscle is being pursued as a possible treatment for DMD. Mesenchymal stem cells have the potential to differentiate and display a myogenic phenotype in vitro. Since liposuctioned human fat is available in large quantities, it may be an ideal source of stem cells for therapeutic applications. ASCs (adipose-derived stem cells) are able to restore dystrophin expression in the muscles of mdx (X-linked muscular dystrophy) mice. However, the outcome when these cells interact with human dystrophic muscle is still unknown.

RESULTS

We show here that ASCs participate in myotube formation when cultured together with differentiating human DMD myoblasts, resulting in the restoration of dystrophin expression. Similarly, dystrophin was induced when ASCs were co-cultivated with DMD myotubes. Experiments with GFP (green fluorescent protein)-positive ASCs and DAPI (4',6-diamidino-2-phenylindole)-stained DMD myoblasts indicated that ASCs participate in human myogenesis through cellular fusion.

CONCLUSIONS

These results show that ASCs have the potential to interact with dystrophic muscle cells, restoring dystrophin expression of DMD cells in vitro. The possibility of using adipose tissue as a source of stem cell therapies for muscular diseases is extremely exciting.

Authors+Show Affiliations

Human Genome Research Center, Biosciences Institute, University of São Paulo, Rua do Matão, CEP 05508-090, n.106 Cidade Universitária, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17997718

Citation

Vieira, Natássia M., et al. "Human Multipotent Adipose-derived Stem Cells Restore Dystrophin Expression of Duchenne Skeletal-muscle Cells in Vitro." Biology of the Cell, vol. 100, no. 4, 2008, pp. 231-41.
Vieira NM, Brandalise V, Zucconi E, et al. Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vitro. Biol Cell. 2008;100(4):231-41.
Vieira, N. M., Brandalise, V., Zucconi, E., Jazedje, T., Secco, M., Nunes, V. A., Strauss, B. E., Vainzof, M., & Zatz, M. (2008). Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vitro. Biology of the Cell, 100(4), 231-41.
Vieira NM, et al. Human Multipotent Adipose-derived Stem Cells Restore Dystrophin Expression of Duchenne Skeletal-muscle Cells in Vitro. Biol Cell. 2008;100(4):231-41. PubMed PMID: 17997718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vitro. AU - Vieira,Natássia M, AU - Brandalise,Vanessa, AU - Zucconi,Eder, AU - Jazedje,Tatiana, AU - Secco,Mariane, AU - Nunes,Viviane A, AU - Strauss,Bryan E, AU - Vainzof,Mariz, AU - Zatz,Mayana, PY - 2007/11/14/pubmed PY - 2008/4/23/medline PY - 2007/11/14/entrez SP - 231 EP - 41 JF - Biology of the cell JO - Biol. Cell VL - 100 IS - 4 N2 - BACKGROUND INFORMATION: DMD (Duchenne muscular dystrophy) is a devastating X-linked disorder characterized by progressive muscle degeneration and weakness. The use of cell therapy for the repair of defective muscle is being pursued as a possible treatment for DMD. Mesenchymal stem cells have the potential to differentiate and display a myogenic phenotype in vitro. Since liposuctioned human fat is available in large quantities, it may be an ideal source of stem cells for therapeutic applications. ASCs (adipose-derived stem cells) are able to restore dystrophin expression in the muscles of mdx (X-linked muscular dystrophy) mice. However, the outcome when these cells interact with human dystrophic muscle is still unknown. RESULTS: We show here that ASCs participate in myotube formation when cultured together with differentiating human DMD myoblasts, resulting in the restoration of dystrophin expression. Similarly, dystrophin was induced when ASCs were co-cultivated with DMD myotubes. Experiments with GFP (green fluorescent protein)-positive ASCs and DAPI (4',6-diamidino-2-phenylindole)-stained DMD myoblasts indicated that ASCs participate in human myogenesis through cellular fusion. CONCLUSIONS: These results show that ASCs have the potential to interact with dystrophic muscle cells, restoring dystrophin expression of DMD cells in vitro. The possibility of using adipose tissue as a source of stem cell therapies for muscular diseases is extremely exciting. SN - 1768-322X UR - https://www.unboundmedicine.com/medline/citation/17997718/Human_multipotent_adipose_derived_stem_cells_restore_dystrophin_expression_of_Duchenne_skeletal_muscle_cells_in_vitro_ L2 - https://doi.org/10.1042/BC20070102 DB - PRIME DP - Unbound Medicine ER -