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Flk-1+ adipose-derived mesenchymal stem cells differentiate into skeletal muscle satellite cells and ameliorate muscular dystrophy in mdx mice.
Stem Cells Dev. 2007 Oct; 16(5):695-706.SC

Abstract

Duchenne muscular dystrophy (DMD) is a severe hereditary disease characterized by the absence of dystrophin on the sarcolemma of muscle fiber. This absence results in widespread muscle damage and satellite cell activation. After depletion of the satellite cell pool, skeletal muscle is then invariably replaced by connective tissue, leading to progressive muscle weakness. Herein, we isolated Flk-1(+) mesenchymal stem cells (MSCs) from adult adipose tissue and induced them to differentiate into skeletal muscle cells in culture. Within mdx mice, an animal model of DMD, adipose tissue-derived Flk-1(+) MSCs (AD-MSCs) homed to and differentiated into cells that repaired injured muscle tissue. This repair correlated with reconstitution of dystrophin expression on the damaged fibers. Flk-1(+) AD-MSCs also differentiated into muscle satellite cells. This differentiation may have accounted for long-term reconstitution. These cells also differentiated into endothelial cells, thereby possibly improving fiber regeneration as a result of the induced angiogenesis. Therefore, Flk-1(+) AD-MSC transplants may repair muscular dystrophy.

Authors+Show Affiliations

Institute of Basic Medical Sciences & School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17999592

Citation

Liu, Yanning, et al. "Flk-1+ Adipose-derived Mesenchymal Stem Cells Differentiate Into Skeletal Muscle Satellite Cells and Ameliorate Muscular Dystrophy in Mdx Mice." Stem Cells and Development, vol. 16, no. 5, 2007, pp. 695-706.
Liu Y, Yan X, Sun Z, et al. Flk-1+ adipose-derived mesenchymal stem cells differentiate into skeletal muscle satellite cells and ameliorate muscular dystrophy in mdx mice. Stem Cells Dev. 2007;16(5):695-706.
Liu, Y., Yan, X., Sun, Z., Chen, B., Han, Q., Li, J., & Zhao, R. C. (2007). Flk-1+ adipose-derived mesenchymal stem cells differentiate into skeletal muscle satellite cells and ameliorate muscular dystrophy in mdx mice. Stem Cells and Development, 16(5), 695-706.
Liu Y, et al. Flk-1+ Adipose-derived Mesenchymal Stem Cells Differentiate Into Skeletal Muscle Satellite Cells and Ameliorate Muscular Dystrophy in Mdx Mice. Stem Cells Dev. 2007;16(5):695-706. PubMed PMID: 17999592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flk-1+ adipose-derived mesenchymal stem cells differentiate into skeletal muscle satellite cells and ameliorate muscular dystrophy in mdx mice. AU - Liu,Yanning, AU - Yan,Xi, AU - Sun,Zhao, AU - Chen,Bin, AU - Han,Qin, AU - Li,Jing, AU - Zhao,Robert Chunhua, PY - 2007/11/15/pubmed PY - 2008/1/11/medline PY - 2007/11/15/entrez SP - 695 EP - 706 JF - Stem cells and development JO - Stem Cells Dev VL - 16 IS - 5 N2 - Duchenne muscular dystrophy (DMD) is a severe hereditary disease characterized by the absence of dystrophin on the sarcolemma of muscle fiber. This absence results in widespread muscle damage and satellite cell activation. After depletion of the satellite cell pool, skeletal muscle is then invariably replaced by connective tissue, leading to progressive muscle weakness. Herein, we isolated Flk-1(+) mesenchymal stem cells (MSCs) from adult adipose tissue and induced them to differentiate into skeletal muscle cells in culture. Within mdx mice, an animal model of DMD, adipose tissue-derived Flk-1(+) MSCs (AD-MSCs) homed to and differentiated into cells that repaired injured muscle tissue. This repair correlated with reconstitution of dystrophin expression on the damaged fibers. Flk-1(+) AD-MSCs also differentiated into muscle satellite cells. This differentiation may have accounted for long-term reconstitution. These cells also differentiated into endothelial cells, thereby possibly improving fiber regeneration as a result of the induced angiogenesis. Therefore, Flk-1(+) AD-MSC transplants may repair muscular dystrophy. SN - 1547-3287 UR - https://www.unboundmedicine.com/medline/citation/17999592/Flk_1+_adipose_derived_mesenchymal_stem_cells_differentiate_into_skeletal_muscle_satellite_cells_and_ameliorate_muscular_dystrophy_in_mdx_mice_ L2 - https://www.liebertpub.com/doi/10.1089/scd.2006.0118?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -