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Human (non-severe acute respiratory syndrome) coronavirus infections in hospitalised children in France.
J Paediatr Child Health. 2008 Apr; 44(4):176-81.JP

Abstract

AIM

This study has two objectives: to study the clinical symptoms associated with the detection of the four human coronaviruses (HCoVs), 229E, OC43, NL63 and HKU1 types, in the respiratory specimens sampled from hospitalised children in France between September 2004 and May 2005; and to develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay allowing for the simultaneous detection of the four HCoVs.

METHODS

1002 respiratory specimens were tested for HCoVs. The clinical and epidemiological data were compared on the basis of the type HCoV infection.

RESULTS

A hundred coronaviruses, 33 NL63, 2229E, 27 OC43 and 38 HKU1, were detected in 97 (9.8%) of 1002 samples negative in routine tests. The clinical and epidemiological characteristics of the study children were compared in three groups, 24 OC43-, 27 NL63- and 34 HKU1-infected children. HCoVs were identified mainly in children with upper and lower respiratory tract infections (50.5% vs. 29.4%). The significant difference in clinical presentation between the three coronavirus groups was the very low association between lower respiratory tract illness and HKU1 detection.

CONCLUSIONS

HCoV detection in hospitalised children without any other respiratory virus detection was associated with upper and a significant rate of lower respiratory tract illness. The four types of HCoVs were detected, and new types NL63 and HKU1 represented a substantial portion of detection. The multiplex RT-PCR enabled a sensitive one-time detection and the characterisation of all of the known HCoV types with the exception severe acute respiratory syndrome-coronavirus.

Authors+Show Affiliations

Laboratory of Virology, University Hospital of Caen, Caen, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17999671

Citation

Vabret, Astrid, et al. "Human (non-severe Acute Respiratory Syndrome) Coronavirus Infections in Hospitalised Children in France." Journal of Paediatrics and Child Health, vol. 44, no. 4, 2008, pp. 176-81.
Vabret A, Dina J, Gouarin S, et al. Human (non-severe acute respiratory syndrome) coronavirus infections in hospitalised children in France. J Paediatr Child Health. 2008;44(4):176-81.
Vabret, A., Dina, J., Gouarin, S., Petitjean, J., Tripey, V., Brouard, J., & Freymuth, F. (2008). Human (non-severe acute respiratory syndrome) coronavirus infections in hospitalised children in France. Journal of Paediatrics and Child Health, 44(4), 176-81.
Vabret A, et al. Human (non-severe Acute Respiratory Syndrome) Coronavirus Infections in Hospitalised Children in France. J Paediatr Child Health. 2008;44(4):176-81. PubMed PMID: 17999671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human (non-severe acute respiratory syndrome) coronavirus infections in hospitalised children in France. AU - Vabret,Astrid, AU - Dina,Julia, AU - Gouarin,Stéphanie, AU - Petitjean,Joëlle, AU - Tripey,Valérie, AU - Brouard,Jacques, AU - Freymuth,François, Y1 - 2007/11/12/ PY - 2007/11/15/pubmed PY - 2008/5/28/medline PY - 2007/11/15/entrez SP - 176 EP - 81 JF - Journal of paediatrics and child health JO - J Paediatr Child Health VL - 44 IS - 4 N2 - AIM: This study has two objectives: to study the clinical symptoms associated with the detection of the four human coronaviruses (HCoVs), 229E, OC43, NL63 and HKU1 types, in the respiratory specimens sampled from hospitalised children in France between September 2004 and May 2005; and to develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay allowing for the simultaneous detection of the four HCoVs. METHODS: 1002 respiratory specimens were tested for HCoVs. The clinical and epidemiological data were compared on the basis of the type HCoV infection. RESULTS: A hundred coronaviruses, 33 NL63, 2229E, 27 OC43 and 38 HKU1, were detected in 97 (9.8%) of 1002 samples negative in routine tests. The clinical and epidemiological characteristics of the study children were compared in three groups, 24 OC43-, 27 NL63- and 34 HKU1-infected children. HCoVs were identified mainly in children with upper and lower respiratory tract infections (50.5% vs. 29.4%). The significant difference in clinical presentation between the three coronavirus groups was the very low association between lower respiratory tract illness and HKU1 detection. CONCLUSIONS: HCoV detection in hospitalised children without any other respiratory virus detection was associated with upper and a significant rate of lower respiratory tract illness. The four types of HCoVs were detected, and new types NL63 and HKU1 represented a substantial portion of detection. The multiplex RT-PCR enabled a sensitive one-time detection and the characterisation of all of the known HCoV types with the exception severe acute respiratory syndrome-coronavirus. SN - 1440-1754 UR - https://www.unboundmedicine.com/medline/citation/17999671/Human__non_severe_acute_respiratory_syndrome__coronavirus_infections_in_hospitalised_children_in_France_ L2 - https://doi.org/10.1111/j.1440-1754.2007.01246.x DB - PRIME DP - Unbound Medicine ER -