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Effects of intrathecal injection of prednisolone acetate on expression of NR2B subunit and nNOS in spinal cord of rats after chronic compression of dorsal root ganglia.
Ann Clin Lab Sci. 2007 Autumn; 37(4):349-55.AC

Abstract

N-methyl-D-aspartate receptor subunit 2B (NR2B) and neuronal nitric oxide synthase (nNOS) play important roles in the mechanism of neuropathic pain. To elucidate how glucocorticoids affect this mechanism, we studied the effects of intrathecal (it) injection of prednisolone acetate (PA) on a nociceptive stimulus and the changes of nNOS and NR2B subunit expression in the spinal dorsal horn of Sprague Dawley rats following chronic compression of the dorsal root ganglia (CCD). Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured for 15 days postoperatively. An it injection of PA (2.0 mg/kg) every 3 days for postoperative days 1 to 15 inhibited the thermal hyperalgesia and tactile allodynia of CCD rats. Chronic compression of the dorsal root ganglia induced time-dependent upregulation of nNOS and NR2B subunits of N-methyl-D-aspartate receptor within the spinal cord dorsal horn ipsilateral to CCD. Both upregulations were significantly diminished by it administration of PA (2.0 mg/kg), but not by lower doses of PA (0.5 or 1.0 mg/kg). The results suggest that PA upregulation of neuronal nitric oxide synthase and NR2B subunit expression in the spinal dorsal horn contributes to PA inhibition of hyperalgesia induced by chronic compression of dorsal root ganglia.

Authors+Show Affiliations

Department of Anesthesiology, Drum Tower Hospital, Nanjing University, Nanjing 210008, Jiangsu Province, China. mazhengliang1964@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18000292

Citation

Ma, Zheng-Liang, et al. "Effects of Intrathecal Injection of Prednisolone Acetate On Expression of NR2B Subunit and nNOS in Spinal Cord of Rats After Chronic Compression of Dorsal Root Ganglia." Annals of Clinical and Laboratory Science, vol. 37, no. 4, 2007, pp. 349-55.
Ma ZL, Zhang W, Gu XP, et al. Effects of intrathecal injection of prednisolone acetate on expression of NR2B subunit and nNOS in spinal cord of rats after chronic compression of dorsal root ganglia. Ann Clin Lab Sci. 2007;37(4):349-55.
Ma, Z. L., Zhang, W., Gu, X. P., Yang, W. S., & Zeng, Y. M. (2007). Effects of intrathecal injection of prednisolone acetate on expression of NR2B subunit and nNOS in spinal cord of rats after chronic compression of dorsal root ganglia. Annals of Clinical and Laboratory Science, 37(4), 349-55.
Ma ZL, et al. Effects of Intrathecal Injection of Prednisolone Acetate On Expression of NR2B Subunit and nNOS in Spinal Cord of Rats After Chronic Compression of Dorsal Root Ganglia. Ann Clin Lab Sci. 2007;37(4):349-55. PubMed PMID: 18000292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of intrathecal injection of prednisolone acetate on expression of NR2B subunit and nNOS in spinal cord of rats after chronic compression of dorsal root ganglia. AU - Ma,Zheng-Liang, AU - Zhang,Wei, AU - Gu,Xiao-Ping, AU - Yang,Wen-Sheng, AU - Zeng,Yin-Ming, PY - 2007/11/15/pubmed PY - 2008/2/21/medline PY - 2007/11/15/entrez SP - 349 EP - 55 JF - Annals of clinical and laboratory science JO - Ann Clin Lab Sci VL - 37 IS - 4 N2 - N-methyl-D-aspartate receptor subunit 2B (NR2B) and neuronal nitric oxide synthase (nNOS) play important roles in the mechanism of neuropathic pain. To elucidate how glucocorticoids affect this mechanism, we studied the effects of intrathecal (it) injection of prednisolone acetate (PA) on a nociceptive stimulus and the changes of nNOS and NR2B subunit expression in the spinal dorsal horn of Sprague Dawley rats following chronic compression of the dorsal root ganglia (CCD). Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured for 15 days postoperatively. An it injection of PA (2.0 mg/kg) every 3 days for postoperative days 1 to 15 inhibited the thermal hyperalgesia and tactile allodynia of CCD rats. Chronic compression of the dorsal root ganglia induced time-dependent upregulation of nNOS and NR2B subunits of N-methyl-D-aspartate receptor within the spinal cord dorsal horn ipsilateral to CCD. Both upregulations were significantly diminished by it administration of PA (2.0 mg/kg), but not by lower doses of PA (0.5 or 1.0 mg/kg). The results suggest that PA upregulation of neuronal nitric oxide synthase and NR2B subunit expression in the spinal dorsal horn contributes to PA inhibition of hyperalgesia induced by chronic compression of dorsal root ganglia. SN - 0091-7370 UR - https://www.unboundmedicine.com/medline/citation/18000292/Effects_of_intrathecal_injection_of_prednisolone_acetate_on_expression_of_NR2B_subunit_and_nNOS_in_spinal_cord_of_rats_after_chronic_compression_of_dorsal_root_ganglia_ L2 - http://www.annclinlabsci.org/cgi/pmidlookup?view=long&pmid=18000292 DB - PRIME DP - Unbound Medicine ER -