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In situ analysis by microspectroscopy reveals triterpenoid compositional patterns within leaf cuticles of Prunus laurocerasus.
Planta 2008; 227(4):823-34P

Abstract

The cuticular waxes on the leaves of Prunus laurocerasus are arranged in distinct layers differing in triterpenoid concentrations (Jetter et al., Plant Cell Environ 23:619-628, 2000). In addition to this transversal gradient, the lateral distribution of cuticular triterpenoids must be investigated to fully describe the spatial distribution of wax components on the leaf surfaces. In the present investigation, near infrared (NIR) Raman microspectroscopy, coherent anti-Stokes Raman scattering (CARS) microscopy, and third harmonic generation (THG) spectroscopy were employed to map the triterpenoid distribution in isolated cuticles from adaxial and abaxial sides of P. laurocerasus leaves. The relative concentrations of ursolic acid and oleanolic acid were calculated by treating the cuticle spectra as linear combinations of reference spectra from the major compounds found in the wax. Raman maps of the adaxial cuticle showed that the triterpenoids accumulate to relatively high concentrations over the periclinal regions of the pavement cells, while the very long chain aliphatic wax constituents are distributed fairly evenly across the entire adaxial cuticle. In the analysis of the abaxial cuticles, the triterpenoids were found to accumulate in greater amounts over the guard cells relative to the pavement cells. The very long chain aliphatic compounds accumulated in the cuticle above the anticlinal cell walls of the pavement cells, and were found at low concentrations above the periclinals and the guard cells.

Authors+Show Affiliations

Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z3.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18000679

Citation

Yu, Marcia M L., et al. "In Situ Analysis By Microspectroscopy Reveals Triterpenoid Compositional Patterns Within Leaf Cuticles of Prunus Laurocerasus." Planta, vol. 227, no. 4, 2008, pp. 823-34.
Yu MM, Konorov SO, Schulze HG, et al. In situ analysis by microspectroscopy reveals triterpenoid compositional patterns within leaf cuticles of Prunus laurocerasus. Planta. 2008;227(4):823-34.
Yu, M. M., Konorov, S. O., Schulze, H. G., Blades, M. W., Turner, R. F., & Jetter, R. (2008). In situ analysis by microspectroscopy reveals triterpenoid compositional patterns within leaf cuticles of Prunus laurocerasus. Planta, 227(4), pp. 823-34.
Yu MM, et al. In Situ Analysis By Microspectroscopy Reveals Triterpenoid Compositional Patterns Within Leaf Cuticles of Prunus Laurocerasus. Planta. 2008;227(4):823-34. PubMed PMID: 18000679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ analysis by microspectroscopy reveals triterpenoid compositional patterns within leaf cuticles of Prunus laurocerasus. AU - Yu,Marcia M L, AU - Konorov,Stanislav O, AU - Schulze,H Georg, AU - Blades,Michael W, AU - Turner,Robin F B, AU - Jetter,Reinhard, Y1 - 2007/11/14/ PY - 2007/05/25/received PY - 2007/10/22/accepted PY - 2007/11/15/pubmed PY - 2008/7/22/medline PY - 2007/11/15/entrez SP - 823 EP - 34 JF - Planta JO - Planta VL - 227 IS - 4 N2 - The cuticular waxes on the leaves of Prunus laurocerasus are arranged in distinct layers differing in triterpenoid concentrations (Jetter et al., Plant Cell Environ 23:619-628, 2000). In addition to this transversal gradient, the lateral distribution of cuticular triterpenoids must be investigated to fully describe the spatial distribution of wax components on the leaf surfaces. In the present investigation, near infrared (NIR) Raman microspectroscopy, coherent anti-Stokes Raman scattering (CARS) microscopy, and third harmonic generation (THG) spectroscopy were employed to map the triterpenoid distribution in isolated cuticles from adaxial and abaxial sides of P. laurocerasus leaves. The relative concentrations of ursolic acid and oleanolic acid were calculated by treating the cuticle spectra as linear combinations of reference spectra from the major compounds found in the wax. Raman maps of the adaxial cuticle showed that the triterpenoids accumulate to relatively high concentrations over the periclinal regions of the pavement cells, while the very long chain aliphatic wax constituents are distributed fairly evenly across the entire adaxial cuticle. In the analysis of the abaxial cuticles, the triterpenoids were found to accumulate in greater amounts over the guard cells relative to the pavement cells. The very long chain aliphatic compounds accumulated in the cuticle above the anticlinal cell walls of the pavement cells, and were found at low concentrations above the periclinals and the guard cells. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/18000679/In_situ_analysis_by_microspectroscopy_reveals_triterpenoid_compositional_patterns_within_leaf_cuticles_of_Prunus_laurocerasus_ L2 - https://dx.doi.org/10.1007/s00425-007-0659-z DB - PRIME DP - Unbound Medicine ER -