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Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle.
J Cell Sci. 2007 Dec 01; 120(Pt 23):4178-87.JC

Abstract

Satellite cells are usually mitotically quiescent muscle stem cells, located between the sarcolemma and the basement membrane of muscle fibers. When muscles are damaged, satellite cells become activated, proliferate and differentiate to form multinucleate myofibers. The molecular mechanisms underlying these processes are poorly understood. In the present study, we found that, following treatment with cardiotoxin, homeodomain-containing transcription factor Lbx1 was strongly expressed in the satellite cells of regenerating adult skeletal muscle. Our immunohistochemical and northern blot analyses indicate that Lbx1 is expressed in activated but not quiescent satellite cells. In vitro, this Lbx1 expression was gradually downregulated when satellite cells differentiate into mature myofibers. Transfection and forced expression of Lbx1 in satellite-cell-derived C2C12 myoblast cells resulted in severe depression of myogenic differentiation and incomplete myotube formation, concomitantly with the activation of the paired-box transcription factor Pax7 and depression of the myogenic regulatory factor MyoD. Moreover, knockdown of Lbx1 in in-vitro-cultured satellite cells resulted in downregulation of Pax7. These results suggest that Lbx1 plays important roles in differentiation and maintenance of satellite cells of mature myofibers, probably through the regulation of Pax7.

Authors+Show Affiliations

Molecular Embryology, Department of Biosciences, School of Science, Kitasato University, Kanagawa, 228-8555, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18003701

Citation

Watanabe, Shuichi, et al. "Functional Analysis of Homeodomain-containing Transcription Factor Lbx1 in Satellite Cells of Mouse Skeletal Muscle." Journal of Cell Science, vol. 120, no. Pt 23, 2007, pp. 4178-87.
Watanabe S, Kondo S, Hayasaka M, et al. Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle. J Cell Sci. 2007;120(Pt 23):4178-87.
Watanabe, S., Kondo, S., Hayasaka, M., & Hanaoka, K. (2007). Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle. Journal of Cell Science, 120(Pt 23), 4178-87.
Watanabe S, et al. Functional Analysis of Homeodomain-containing Transcription Factor Lbx1 in Satellite Cells of Mouse Skeletal Muscle. J Cell Sci. 2007 Dec 1;120(Pt 23):4178-87. PubMed PMID: 18003701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle. AU - Watanabe,Shuichi, AU - Kondo,Shunzo, AU - Hayasaka,Michiko, AU - Hanaoka,Kazunori, Y1 - 2007/11/14/ PY - 2007/11/16/pubmed PY - 2008/3/28/medline PY - 2007/11/16/entrez SP - 4178 EP - 87 JF - Journal of cell science JO - J Cell Sci VL - 120 IS - Pt 23 N2 - Satellite cells are usually mitotically quiescent muscle stem cells, located between the sarcolemma and the basement membrane of muscle fibers. When muscles are damaged, satellite cells become activated, proliferate and differentiate to form multinucleate myofibers. The molecular mechanisms underlying these processes are poorly understood. In the present study, we found that, following treatment with cardiotoxin, homeodomain-containing transcription factor Lbx1 was strongly expressed in the satellite cells of regenerating adult skeletal muscle. Our immunohistochemical and northern blot analyses indicate that Lbx1 is expressed in activated but not quiescent satellite cells. In vitro, this Lbx1 expression was gradually downregulated when satellite cells differentiate into mature myofibers. Transfection and forced expression of Lbx1 in satellite-cell-derived C2C12 myoblast cells resulted in severe depression of myogenic differentiation and incomplete myotube formation, concomitantly with the activation of the paired-box transcription factor Pax7 and depression of the myogenic regulatory factor MyoD. Moreover, knockdown of Lbx1 in in-vitro-cultured satellite cells resulted in downregulation of Pax7. These results suggest that Lbx1 plays important roles in differentiation and maintenance of satellite cells of mature myofibers, probably through the regulation of Pax7. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/18003701/Functional_analysis_of_homeodomain_containing_transcription_factor_Lbx1_in_satellite_cells_of_mouse_skeletal_muscle_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=18003701 DB - PRIME DP - Unbound Medicine ER -