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A simple and rapid determination of valproic acid in human plasma using a non-porous silica column and liquid chromatography with tandem mass spectrometric detection.
Biomed Chromatogr. 2008 Apr; 22(4):387-93.BC

Abstract

A liquid chromatographic tandem mass spectrometric (LC-MS/MS) assay was developed and validated to determine valproic acid in human plasma. The method involved a solid-phase extraction of valproic acid and betamethasone valerate, an internal standard, from plasma and detection using an LC-MS/MS system with electrospray ionization source in negative ion mode. Separation was achieved within 3 min on a non-porous silica column with mobile phase containing ammonium acetate and methanol. Multiple reaction monitoring was utilized for detection monitoring at 142.89-142.89 for valproic acid and 457.21-457.21 for the internal standard. The calibration curve for valproic acid was linear over the range of 0.5-150 microg/mL. The limit of detection was 0.17 microg/mL and the lower limit of quantification was 0.5 microg/mL, when 0.2 mL plasma was used for extraction. The percentage coefficient of validation for accuracy and precision (inter- and intra-day) for this method was less than 9.5% with recovery ranging from 82.3 to 86.9% for valproic acid.

Authors+Show Affiliations

Department of Pharmacy, Gifu University Hospital, Gifu Pharmaceutical University, Gifu, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18004739

Citation

Matsuura, Katsuhiko, et al. "A Simple and Rapid Determination of Valproic Acid in Human Plasma Using a Non-porous Silica Column and Liquid Chromatography With Tandem Mass Spectrometric Detection." Biomedical Chromatography : BMC, vol. 22, no. 4, 2008, pp. 387-93.
Matsuura K, Ohmori T, Nakamura M, et al. A simple and rapid determination of valproic acid in human plasma using a non-porous silica column and liquid chromatography with tandem mass spectrometric detection. Biomed Chromatogr. 2008;22(4):387-93.
Matsuura, K., Ohmori, T., Nakamura, M., Itoh, Y., & Hirano, K. (2008). A simple and rapid determination of valproic acid in human plasma using a non-porous silica column and liquid chromatography with tandem mass spectrometric detection. Biomedical Chromatography : BMC, 22(4), 387-93.
Matsuura K, et al. A Simple and Rapid Determination of Valproic Acid in Human Plasma Using a Non-porous Silica Column and Liquid Chromatography With Tandem Mass Spectrometric Detection. Biomed Chromatogr. 2008;22(4):387-93. PubMed PMID: 18004739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple and rapid determination of valproic acid in human plasma using a non-porous silica column and liquid chromatography with tandem mass spectrometric detection. AU - Matsuura,Katsuhiko, AU - Ohmori,Tomofumi, AU - Nakamura,Mitsuhiro, AU - Itoh,Yoshinori, AU - Hirano,Kazuyuki, PY - 2007/11/16/pubmed PY - 2008/7/1/medline PY - 2007/11/16/entrez SP - 387 EP - 93 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 22 IS - 4 N2 - A liquid chromatographic tandem mass spectrometric (LC-MS/MS) assay was developed and validated to determine valproic acid in human plasma. The method involved a solid-phase extraction of valproic acid and betamethasone valerate, an internal standard, from plasma and detection using an LC-MS/MS system with electrospray ionization source in negative ion mode. Separation was achieved within 3 min on a non-porous silica column with mobile phase containing ammonium acetate and methanol. Multiple reaction monitoring was utilized for detection monitoring at 142.89-142.89 for valproic acid and 457.21-457.21 for the internal standard. The calibration curve for valproic acid was linear over the range of 0.5-150 microg/mL. The limit of detection was 0.17 microg/mL and the lower limit of quantification was 0.5 microg/mL, when 0.2 mL plasma was used for extraction. The percentage coefficient of validation for accuracy and precision (inter- and intra-day) for this method was less than 9.5% with recovery ranging from 82.3 to 86.9% for valproic acid. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/18004739/A_simple_and_rapid_determination_of_valproic_acid_in_human_plasma_using_a_non_porous_silica_column_and_liquid_chromatography_with_tandem_mass_spectrometric_detection_ L2 - https://doi.org/10.1002/bmc.944 DB - PRIME DP - Unbound Medicine ER -