Tags

Type your tag names separated by a space and hit enter

Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm Aerobic Count plates and conventional pour plate methodology.
J Dairy Res. 2007 Nov; 74(4):387-91.JD

Abstract

This study aimed to compare Petrifilm Aerobic Count (AC) plates and the conventional pour plate methodology using de Mann-Rogosa-Sharpe (MRS), Kang-Fung (KF) and Kang-Fung-Sol (KFS) culture media for screening and enumeration of lactic acid bacteria (LAB) in milk. Suspensions of 10 LAB species in reconstituted powder skim milk and 30 raw milk samples, without experimental inoculation, were tested. For selective enumeration, all samples were previously diluted in MRS, KF and KFS broths and then plated in Petrifilm AC and conventional pour plate methodology, using the same culture media with added agar. All plates were incubated at 37 degrees C for 48 h in anaerobic conditions. Differences in the counts were observed only for raw milk samples using KFS in conventional methodology, when compared with the counts obtained from MRS and KF (P0.05). The results showed excellent correlation indexes between both methodologies using the three culture media for LAB suspensions (r=0.97 for MRS, KF and KFS). For raw milk samples, the correlation indexes were excellent (r=0.97, for MRS) and good (r=0.84 for KF, and r=0.82 for KFS), showing some interference in Petrifilm AC when supplements were added, especially lactic acid. These results indicate the possibility of using Petrifilm AC plates for enumeration of LAB in milk, even with the use of selective supplements.

Authors+Show Affiliations

Departamento de Veterinária, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18005460

Citation

Ortolani, Maria B T., et al. "Screening and Enumeration of Lactic Acid Bacteria in Milk Using Three Different Culture Media in Petrifilm Aerobic Count Plates and Conventional Pour Plate Methodology." The Journal of Dairy Research, vol. 74, no. 4, 2007, pp. 387-91.
Ortolani MB, Viçosa GN, Beloti V, et al. Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm Aerobic Count plates and conventional pour plate methodology. J Dairy Res. 2007;74(4):387-91.
Ortolani, M. B., Viçosa, G. N., Beloti, V., & Nero, L. A. (2007). Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm Aerobic Count plates and conventional pour plate methodology. The Journal of Dairy Research, 74(4), 387-91.
Ortolani MB, et al. Screening and Enumeration of Lactic Acid Bacteria in Milk Using Three Different Culture Media in Petrifilm Aerobic Count Plates and Conventional Pour Plate Methodology. J Dairy Res. 2007;74(4):387-91. PubMed PMID: 18005460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm Aerobic Count plates and conventional pour plate methodology. AU - Ortolani,Maria B T, AU - Viçosa,Gabriela N, AU - Beloti,Vanerli, AU - Nero,Luís A, PY - 2007/11/17/pubmed PY - 2008/2/28/medline PY - 2007/11/17/entrez SP - 387 EP - 91 JF - The Journal of dairy research JO - J Dairy Res VL - 74 IS - 4 N2 - This study aimed to compare Petrifilm Aerobic Count (AC) plates and the conventional pour plate methodology using de Mann-Rogosa-Sharpe (MRS), Kang-Fung (KF) and Kang-Fung-Sol (KFS) culture media for screening and enumeration of lactic acid bacteria (LAB) in milk. Suspensions of 10 LAB species in reconstituted powder skim milk and 30 raw milk samples, without experimental inoculation, were tested. For selective enumeration, all samples were previously diluted in MRS, KF and KFS broths and then plated in Petrifilm AC and conventional pour plate methodology, using the same culture media with added agar. All plates were incubated at 37 degrees C for 48 h in anaerobic conditions. Differences in the counts were observed only for raw milk samples using KFS in conventional methodology, when compared with the counts obtained from MRS and KF (P0.05). The results showed excellent correlation indexes between both methodologies using the three culture media for LAB suspensions (r=0.97 for MRS, KF and KFS). For raw milk samples, the correlation indexes were excellent (r=0.97, for MRS) and good (r=0.84 for KF, and r=0.82 for KFS), showing some interference in Petrifilm AC when supplements were added, especially lactic acid. These results indicate the possibility of using Petrifilm AC plates for enumeration of LAB in milk, even with the use of selective supplements. SN - 0022-0299 UR - https://www.unboundmedicine.com/medline/citation/18005460/Screening_and_enumeration_of_lactic_acid_bacteria_in_milk_using_three_different_culture_media_in_Petrifilm_Aerobic_Count_plates_and_conventional_pour_plate_methodology_ DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.