Tags

Type your tag names separated by a space and hit enter

Fatty acid composition of serum lipid classes in mice following allergic sensitisation with or without dietary docosahexaenoic acid-enriched fish oil substitution.
Br J Nutr 2008; 99(6):1239-46BJ

Abstract

Dietary fatty acids have been shown to influence allergic sensitisation. Both n-3 and n-6 PUFA are involved in targeted mediation of inflammatory responses during allergic sensitisation and manifestation of atopic diseases. In the present experiments we investigated whether supplementation of DHA-enriched fish oil partly substituting dietary sunflower-seed oil, in comparison with sunflower-seed oil, supplemented to mice influences fatty acid composition of serum lipid classes. The effects of the two different diets were also investigated depending on allergic sensitisation. Supplementation of DHA and EPA in doses of 2 and 0.12 % (w/w) to non-sensitised and sensitised mice resulted in significantly increased percentile contributions of DHA to all lipid classes. In contrast, serum values of the n-6 PUFA arachidonic acid (AA) were significantly lower, both in non-sensitised and sensitised mice fed the DHA-enriched diet. The fatty acid composition of serum lipids also reflected allergic sensitisation: the EPA:AA ratio in TAG, cholesteryl esters and phospholipids in non-supplemented animals fell to 23, 29 and 29 % respectively of the original value after allergic sensitisation, whereas it decreased to 70, 80 and 76 % respectively only in the animals supplemented with DHA. In summary, allergic sensitisation alone decreased significantly the EPA:AA ratios in serum TAG, while concomitant supplementation of DHA-enriched fish oil ameliorated this decrease. We postulate from the present results that the amelioration of the severity of allergic sensitisation after DHA supplementation may be linked to altered ratios of the eicosanoid precursors EPA and AA as well as DHA needed for further metabolic activation to pro- or anti-inflammatory bioactive lipids.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Medical and Health Science Centre, University of Debrecen, Nagyerdei Krt. 98, H-4012 Debrecen, Hungary. rruehl@dote.huNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18005485

Citation

Rühl, Ralph, et al. "Fatty Acid Composition of Serum Lipid Classes in Mice Following Allergic Sensitisation With or Without Dietary Docosahexaenoic Acid-enriched Fish Oil Substitution." The British Journal of Nutrition, vol. 99, no. 6, 2008, pp. 1239-46.
Rühl R, Koch C, Marosvölgyi T, et al. Fatty acid composition of serum lipid classes in mice following allergic sensitisation with or without dietary docosahexaenoic acid-enriched fish oil substitution. Br J Nutr. 2008;99(6):1239-46.
Rühl, R., Koch, C., Marosvölgyi, T., Mihály, J., Schweigert, F. J., Worm, M., & Decsi, T. (2008). Fatty acid composition of serum lipid classes in mice following allergic sensitisation with or without dietary docosahexaenoic acid-enriched fish oil substitution. The British Journal of Nutrition, 99(6), pp. 1239-46.
Rühl R, et al. Fatty Acid Composition of Serum Lipid Classes in Mice Following Allergic Sensitisation With or Without Dietary Docosahexaenoic Acid-enriched Fish Oil Substitution. Br J Nutr. 2008;99(6):1239-46. PubMed PMID: 18005485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acid composition of serum lipid classes in mice following allergic sensitisation with or without dietary docosahexaenoic acid-enriched fish oil substitution. AU - Rühl,Ralph, AU - Koch,Christin, AU - Marosvölgyi,Tamás, AU - Mihály,Johanna, AU - Schweigert,Florian J, AU - Worm,Margitta, AU - Decsi,Tamás, Y1 - 2007/11/16/ PY - 2007/11/17/pubmed PY - 2008/6/24/medline PY - 2007/11/17/entrez SP - 1239 EP - 46 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 99 IS - 6 N2 - Dietary fatty acids have been shown to influence allergic sensitisation. Both n-3 and n-6 PUFA are involved in targeted mediation of inflammatory responses during allergic sensitisation and manifestation of atopic diseases. In the present experiments we investigated whether supplementation of DHA-enriched fish oil partly substituting dietary sunflower-seed oil, in comparison with sunflower-seed oil, supplemented to mice influences fatty acid composition of serum lipid classes. The effects of the two different diets were also investigated depending on allergic sensitisation. Supplementation of DHA and EPA in doses of 2 and 0.12 % (w/w) to non-sensitised and sensitised mice resulted in significantly increased percentile contributions of DHA to all lipid classes. In contrast, serum values of the n-6 PUFA arachidonic acid (AA) were significantly lower, both in non-sensitised and sensitised mice fed the DHA-enriched diet. The fatty acid composition of serum lipids also reflected allergic sensitisation: the EPA:AA ratio in TAG, cholesteryl esters and phospholipids in non-supplemented animals fell to 23, 29 and 29 % respectively of the original value after allergic sensitisation, whereas it decreased to 70, 80 and 76 % respectively only in the animals supplemented with DHA. In summary, allergic sensitisation alone decreased significantly the EPA:AA ratios in serum TAG, while concomitant supplementation of DHA-enriched fish oil ameliorated this decrease. We postulate from the present results that the amelioration of the severity of allergic sensitisation after DHA supplementation may be linked to altered ratios of the eicosanoid precursors EPA and AA as well as DHA needed for further metabolic activation to pro- or anti-inflammatory bioactive lipids. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18005485/Fatty_acid_composition_of_serum_lipid_classes_in_mice_following_allergic_sensitisation_with_or_without_dietary_docosahexaenoic_acid_enriched_fish_oil_substitution_ L2 - https://www.cambridge.org/core/product/identifier/S0007114507862374/type/journal_article DB - PRIME DP - Unbound Medicine ER -