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Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides.
Carbohydr Res. 2008 Jan 14; 343(1):67-72.CR

Abstract

Isolated cell walls of Argania spinosa fruit pulp were fractionated into their polysaccharide constituents and the resulting fractions were analysed for monosaccharide composition and chemical structure. The data reveal the presence of homogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) in the pectic fraction. RG-I is abundant and contains high amounts of Ara and Gal, indicative of an important branching in this polysaccharide. RG-II is less abundant than RG-I and exists as a dimer. Structural characterisation of xyloglucan using enzymatic hydrolysis, gas chromatography, MALDI-TOF-MS and methylation analysis shows that XXGG, XXXG, XXLG and XLLG are the major subunit oligosaccharides in the ratio of 0.6:1:1.2:1.6. This finding demonstrates that the major neutral hemicellulosic polysaccharide is a galacto-xyloglucan. In addition, Argania fruit xyloglucan has no XUFG, a novel xyloglucan motif recently discovered in Argania leaf cell walls. Finally, the isolation and analysis of arabinogalactan-proteins showed that Argania fruit pulp is rich in these proteoglycans.

Authors+Show Affiliations

Aboughe-Angone S
UMR CNRS 6037, IFRMP23, Université de Rouen, Mont Saint Aignan 76821, France. sophie.abougheangone@etu.univ-rouen.fr
Nguema-Ona E
No affiliation info available
Ghosh P
No affiliation info available
Lerouge P
No affiliation info available
Ishii T
No affiliation info available
Ray B
No affiliation info available
Driouich A
No affiliation info available

MeSH

Carbohydrate SequenceCell WallFruitGlucansMonosaccharidesPectinsPolysaccharidesSapotaceaeXylans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18005949

Citation

Aboughe-Angone, Sophie, et al. "Cell Wall Carbohydrates From Fruit Pulp of Argania Spinosa: Structural Analysis of Pectin and Xyloglucan Polysaccharides." Carbohydrate Research, vol. 343, no. 1, 2008, pp. 67-72.
Aboughe-Angone S, Nguema-Ona E, Ghosh P, et al. Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides. Carbohydr Res. 2008;343(1):67-72.
Aboughe-Angone, S., Nguema-Ona, E., Ghosh, P., Lerouge, P., Ishii, T., Ray, B., & Driouich, A. (2008). Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides. Carbohydrate Research, 343(1), 67-72.
Aboughe-Angone S, et al. Cell Wall Carbohydrates From Fruit Pulp of Argania Spinosa: Structural Analysis of Pectin and Xyloglucan Polysaccharides. Carbohydr Res. 2008 Jan 14;343(1):67-72. PubMed PMID: 18005949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides. AU - Aboughe-Angone,Sophie, AU - Nguema-Ona,Eric, AU - Ghosh,Partha, AU - Lerouge,Patrice, AU - Ishii,Tadashi, AU - Ray,Bimalendu, AU - Driouich,Azeddine, Y1 - 2007/10/30/ PY - 2007/04/23/received PY - 2007/10/08/revised PY - 2007/10/24/accepted PY - 2007/11/17/pubmed PY - 2008/4/25/medline PY - 2007/11/17/entrez SP - 67 EP - 72 JF - Carbohydrate research JO - Carbohydr Res VL - 343 IS - 1 N2 - Isolated cell walls of Argania spinosa fruit pulp were fractionated into their polysaccharide constituents and the resulting fractions were analysed for monosaccharide composition and chemical structure. The data reveal the presence of homogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) in the pectic fraction. RG-I is abundant and contains high amounts of Ara and Gal, indicative of an important branching in this polysaccharide. RG-II is less abundant than RG-I and exists as a dimer. Structural characterisation of xyloglucan using enzymatic hydrolysis, gas chromatography, MALDI-TOF-MS and methylation analysis shows that XXGG, XXXG, XXLG and XLLG are the major subunit oligosaccharides in the ratio of 0.6:1:1.2:1.6. This finding demonstrates that the major neutral hemicellulosic polysaccharide is a galacto-xyloglucan. In addition, Argania fruit xyloglucan has no XUFG, a novel xyloglucan motif recently discovered in Argania leaf cell walls. Finally, the isolation and analysis of arabinogalactan-proteins showed that Argania fruit pulp is rich in these proteoglycans. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/18005949/Cell_wall_carbohydrates_from_fruit_pulp_of_Argania_spinosa:_structural_analysis_of_pectin_and_xyloglucan_polysaccharides_ DB - PRIME DP - Unbound Medicine ER -