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The genotoxic potential of glutaraldehyde in mammalian cells in vitro in comparison with formaldehyde.

Abstract

Glutaraldehyde (GA) induces DNA-protein crosslinks (DPX), but conflicting results have been reported with regard to other genotoxic and mutagenic effects in mammalian cells in vitro. We, therefore, characterized the genotoxic and mutagenic potential of GA in V79 cells. Using the alkaline comet assay we demonstrated the induction of DPX by GA (reduction of gamma ray-induced DNA migration) at a concentration of 10 microM and above. The standard comet assay did not reveal a significant DNA strand-breaking activity of GA. Cross-linking concentrations of GA were also cytotoxic, i.e. inhibited cell growth of treated V79 cultures. Interestingly, a small but statistically significant increase in sister chromatid exchange (SCE) and micronuclei (MN) was already measured at lower concentrations (2 and 5 microM). FISH analysis revealed that the majority of GA-induced MN was due to chromosome breaks. We also compared the genotoxic activity of GA to that of formaldehyde (FA). Similar to GA, FA-induced DPX, SCE and MN, but distinct differences exist with regard to the sensitivity of the endpoints and the relationship between genotoxicity and cytotoxicity. However, the differences in genotoxicity cannot readily explain the different carcinogenic activities of the two compounds.

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  • Authors+Show Affiliations

    ,

    Universität Ulm, Institut für Humangenetik, Ulm, Germany. guenter.speit@uni-ulm.de

    , , ,

    Source

    Mutation research 649:1-2 2008 Jan 8 pg 146-54

    MeSH

    Animals
    Cell Line
    Comet Assay
    DNA Breaks
    DNA Damage
    Formaldehyde
    Glutaral
    In Situ Hybridization, Fluorescence
    Micronucleus Tests
    Sister Chromatid Exchange

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    18006368